Oxidative stress response of tumor cells: microarray-based comparison between artemisinins and anthracyclines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- T Efferth
- F Oesch
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000222195500001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.bcp.2004.03.003
- eISSN
- 1873-2968
- Externe Identifier
- Clarivate Analytics Document Solution ID: 831LI
- PubMed Identifier: 15183112
- ISSN
- 0006-2952
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BIOCHEMICAL PHARMACOLOGY
- Schlüsselwörter
- artemisinin
- anthracyclines
- cardiotoxicity
- cluster analysis
- microarrays
- neurotoxicity
- oxidative stress
- Paginierung
- 3 - 10
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Titel
- Oxidative stress response of tumor cells: microarray-based comparison between artemisinins and anthracyclines
- Sub types
- Article
- Ausgabe der Zeitschrift
- 68
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Thomas Efferth
- Franz Oesch
- DOI
- 10.1016/j.bcp.2004.03.003
- ISSN
- 0006-2952
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Biochemical Pharmacology
- Sprache
- en
- Paginierung
- 3 - 10
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.bcp.2004.03.003
- Datum der Datenerfassung
- 2019
- Titel
- Oxidative stress response of tumor cells: microarray-based comparison between artemisinins and anthracyclines
- Ausgabe der Zeitschrift
- 68
Data source: Crossref
- Abstract
- The antimalarial artemisinins also reveal profound cytotoxic activity against tumor cells. Artemisinins harbor an endoperoxide bridge whose cleavage results in the generation of reactive oxygen species (ROS) and/or artemisinin carbon-centered free radicals. Established cancer drugs such as anthracyclines also form ROS and free radicals that are responsible for the cardiotoxicity of anthracyclines. In contrast, artemisinins do not reveal cardiotoxicity. In the present investigation, we compared the cytotoxic activities of different artemisinins (artemisinin, artesunate, arteether, artemether, artemisitene, dihydroartemisinylester stereoisomers) in 60 cell lines of the National Cancer Institute (NCI), USA, with those of anthracyclines (doxorubicin, daunorubicin, 4'-epirubicin, idarubicin, deoxydoxorubicin, trifluoroacetyl-doxorubicin-14-valerate). The inhibition concentration 50% (IC(50)) values of artemisinins and anthracyclines were correlated with the mRNA expression of 170 genes involved in oxygen stress response and metabolism as recently determined by microarray analysis and deposited in the NCI database (http://dtp.nci.nih.gov). The genes whose expression was significantly linked to cellular drug response in Kendall's tau tests were subjected to hierarchical cluster analysis and cluster image mapping. Mathematical correction for false-positive correlations was done by a false discovery rate algorithm. One cluster contained predominantly genes with a relationship to artemisinins and another one genes with a relationship to anthracyclines. In a third cluster, genes correlating to both drug classes were assembled. This indicates that different sets of genes involved in oxidative stress response and metabolism may contribute to the cytotoxic and differing toxic side effects of these drug classes.
- Addresses
- Center for Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany. thomas.efferth@web.de
- Autoren
- Thomas Efferth
- Thomas Efferth
- Franz Oesch
- DOI
- 10.1016/j.bcp.2004.03.003
- eISSN
- 1873-2968
- Externe Identifier
- PubMed Identifier: 15183112
- Open access
- false
- ISSN
- 0006-2952
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Biochemical pharmacology
- Schlüsselwörter
- Tumor Cells, Cultured
- Animals
- Humans
- Sesquiterpenes
- Artemisinins
- Anthracyclines
- RNA, Messenger
- Antineoplastic Agents
- Antimalarials
- Oligonucleotide Array Sequence Analysis
- Drug Screening Assays, Antitumor
- Cluster Analysis
- Gene Expression Profiling
- Inhibitory Concentration 50
- Oxidation-Reduction
- Oxidative Stress
- Sprache
- eng
- Medium
- Paginierung
- 3 - 10
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Datum der Datenerfassung
- 2004
- Titel
- Oxidative stress response of tumor cells: microarray-based comparison between artemisinins and anthracyclines.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 68
Data source: Europe PubMed Central
- Abstract
- The antimalarial artemisinins also reveal profound cytotoxic activity against tumor cells. Artemisinins harbor an endoperoxide bridge whose cleavage results in the generation of reactive oxygen species (ROS) and/or artemisinin carbon-centered free radicals. Established cancer drugs such as anthracyclines also form ROS and free radicals that are responsible for the cardiotoxicity of anthracyclines. In contrast, artemisinins do not reveal cardiotoxicity. In the present investigation, we compared the cytotoxic activities of different artemisinins (artemisinin, artesunate, arteether, artemether, artemisitene, dihydroartemisinylester stereoisomers) in 60 cell lines of the National Cancer Institute (NCI), USA, with those of anthracyclines (doxorubicin, daunorubicin, 4'-epirubicin, idarubicin, deoxydoxorubicin, trifluoroacetyl-doxorubicin-14-valerate). The inhibition concentration 50% (IC(50)) values of artemisinins and anthracyclines were correlated with the mRNA expression of 170 genes involved in oxygen stress response and metabolism as recently determined by microarray analysis and deposited in the NCI database (http://dtp.nci.nih.gov). The genes whose expression was significantly linked to cellular drug response in Kendall's tau tests were subjected to hierarchical cluster analysis and cluster image mapping. Mathematical correction for false-positive correlations was done by a false discovery rate algorithm. One cluster contained predominantly genes with a relationship to artemisinins and another one genes with a relationship to anthracyclines. In a third cluster, genes correlating to both drug classes were assembled. This indicates that different sets of genes involved in oxidative stress response and metabolism may contribute to the cytotoxic and differing toxic side effects of these drug classes.
- Date of acceptance
- 2004
- Autoren
- Thomas Efferth
- Franz Oesch
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/15183112
- DOI
- 10.1016/j.bcp.2004.03.003
- ISSN
- 0006-2952
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Biochem Pharmacol
- Schlüsselwörter
- Animals
- Anthracyclines
- Antimalarials
- Antineoplastic Agents
- Artemisinins
- Cluster Analysis
- Drug Screening Assays, Antitumor
- Gene Expression Profiling
- Humans
- Inhibitory Concentration 50
- Oligonucleotide Array Sequence Analysis
- Oxidation-Reduction
- Oxidative Stress
- RNA, Messenger
- Sesquiterpenes
- Tumor Cells, Cultured
- Sprache
- eng
- Country
- England
- Paginierung
- 3 - 10
- PII
- S0006295204001777
- Datum der Veröffentlichung
- 2004
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2004
- Titel
- Oxidative stress response of tumor cells: microarray-based comparison between artemisinins and anthracyclines.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 68
Data source: PubMed
- Beziehungen:
- Property of