Activity of novel plant extracts against medullary thyroid carcinoma cells

Publication type:
Journal article
Metadata:
Autoren
  • B Rinner
  • V Siegl
  • P Pürstner
  • T Efferth
  • B Brem
  • H Greger
  • R Pfragner
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000221384900015&DestLinkType=FullRecord&DestApp=WOS_CPL
eISSN
1791-7530
Externe Identifier
  • Clarivate Analytics Document Solution ID: 820JL
  • PubMed Identifier: 15152949
ISSN
0250-7005
Ausgabe der Veröffentlichung
2A
Zeitschrift
ANTICANCER RESEARCH
Schlüsselwörter
  • medullary thyroid carcinoma
  • drug resistance
  • apoptosis
  • plant - based drugs
Paginierung
495 - 500
Datum der Veröffentlichung
2004
Status
Published
Titel
Activity of novel plant extracts against medullary thyroid carcinoma cells
Sub types
  • Article
  • Proceedings Paper
Ausgabe der Zeitschrift
24

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<h4>Background</h4>Medullary thyroid carcinoma (MTC) is a rare calcitonin-producing tumor, derived from the parafollicular C-cells of the thyroid. MTC is known to be relatively insensitive to conventional chemotherapy.<h4>Materials and methods</h4>Eight cell lines were established from MTCs; each showed an up-regulation of Bcl-2. We investigated ten agents from plants of the genera Stemona (Stemonaceae), Aglaia (Meliaceae) and Artemisia (Asteraceae) for their effects on proliferation and apoptotic rates. Extracts have been used in traditional Chinese medicine; however, no experience on their effects on medullary thyroid carcinomas has been reported so far. Growth kinetics and viability were examined using the Casy-1-Cell Counter & Analyzer and the WST-1-based cytotoxicity assay. Apoptosis was studied by DAPI staining, by measurement of caspase-3 activity and Bcl-2 expression.<h4>Results</h4>A strong antiproliferative effect was recognized in each Aglaia species and with Artesunate, whereas an enhancement of apoptosis was provoked particularly by Stemona tuberosa Lour.<h4>Conclusion</h4>The activity of the novel plant extracts possiby offers a new approach towards successful chemotherapy of the so far chemo-resistant medullary thyroid carcinoma.
Addresses
  • Department of Pathophysiology, Medical University of Graz, Heinrichstrasse 31, A-8010 Graz, Austria.
Autoren
  • Beate Rinner
  • Veronika Siegl
  • Peter Pürstner
  • Thomas Efferth
  • Brigitte Brem
  • Harald Greger
  • Roswitha Pfragner
eISSN
1791-7530
Externe Identifier
  • PubMed Identifier: 15152949
Open access
false
ISSN
0250-7005
Ausgabe der Veröffentlichung
2A
Zeitschrift
Anticancer research
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Artemisia
  • Aglaia
  • Stemonaceae
  • Plants, Medicinal
  • Carcinoma, Medullary
  • Thyroid Neoplasms
  • Plant Extracts
  • Phytotherapy
  • Drug Resistance, Neoplasm
  • Magnoliopsida
Sprache
eng
Medium
Print
Paginierung
495 - 500
Datum der Veröffentlichung
2004
Status
Published
Datum der Datenerfassung
2004
Titel
Activity of novel plant extracts against medullary thyroid carcinoma cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
24

Data source: Europe PubMed Central

Abstract
BACKGROUND: Medullary thyroid carcinoma (MTC) is a rare calcitonin-producing tumor, derived from the parafollicular C-cells of the thyroid. MTC is known to be relatively insensitive to conventional chemotherapy. MATERIALS AND METHODS: Eight cell lines were established from MTCs; each showed an up-regulation of Bcl-2. We investigated ten agents from plants of the genera Stemona (Stemonaceae), Aglaia (Meliaceae) and Artemisia (Asteraceae) for their effects on proliferation and apoptotic rates. Extracts have been used in traditional Chinese medicine; however, no experience on their effects on medullary thyroid carcinomas has been reported so far. Growth kinetics and viability were examined using the Casy-1-Cell Counter & Analyzer and the WST-1-based cytotoxicity assay. Apoptosis was studied by DAPI staining, by measurement of caspase-3 activity and Bcl-2 expression. RESULTS: A strong antiproliferative effect was recognized in each Aglaia species and with Artesunate, whereas an enhancement of apoptosis was provoked particularly by Stemona tuberosa Lour. CONCLUSION: The activity of the novel plant extracts possiby offers a new approach towards successful chemotherapy of the so far chemo-resistant medullary thyroid carcinoma.
Autoren
  • Beate Rinner
  • Veronika Siegl
  • Peter Pürstner
  • Thomas Efferth
  • Brigitte Brem
  • Harald Greger
  • Roswitha Pfragner
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/15152949
ISSN
0250-7005
Ausgabe der Veröffentlichung
2A
Zeitschrift
Anticancer Res
Schlüsselwörter
  • Aglaia
  • Artemisia
  • Carcinoma, Medullary
  • Cell Line, Tumor
  • Drug Resistance, Neoplasm
  • Humans
  • Magnoliopsida
  • Phytotherapy
  • Plant Extracts
  • Plants, Medicinal
  • Stemonaceae
  • Thyroid Neoplasms
Sprache
eng
Country
Greece
Paginierung
495 - 500
Datum der Veröffentlichung
2004
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2004
Titel
Activity of novel plant extracts against medullary thyroid carcinoma cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
24

Data source: PubMed

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