Microarray-based detection of multidrug resistance in human tumor cells by expression profiling of ATP-binding cassette transporter genes

Publication type:
Journal article
Metadata:
Autoren
  • JP Gillet
  • T Efferth
  • D Steinbach
  • J Hamels
  • F de Longueville
  • V Bertholet
  • J Remacle
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000225809200031&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1158/0008-5472.CAN-04-1978
Externe Identifier
  • Clarivate Analytics Document Solution ID: 880SJ
  • PubMed Identifier: 15604263
ISSN
0008-5472
Ausgabe der Veröffentlichung
24
Zeitschrift
CANCER RESEARCH
Paginierung
8987 - 8993
Datum der Veröffentlichung
2004
Status
Published
Titel
Microarray-based detection of multidrug resistance in human tumor cells by expression profiling of ATP-binding cassette transporter genes
Sub types
  • Article
Ausgabe der Zeitschrift
64

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title> <jats:p>Different mechanisms of drug resistance, including ATP-binding cassette (ABC) transporters, are responsible for treatment failure of tumors. We developed a low-density DNA microarray which contains 38 genes of the ABC transporter gene family. This tool has been validated with three different multidrug-resistant sublines (CEM/ADR5000, HL60/AR, and MCF7/CH1000) known to overexpress either the ABCB1 (MDR1), ABCC1 (MRP1), or ABCG2 (MXR and BCRP) genes. When compared with their drug-sensitive parental lines, we observed not only the overexpression of these genes in the multidrug-resistant cell lines but also of other ABC transporter genes pointing to their possible role in multidrug resistance. These results were corroborated by quantitative real-time reverse transcription-PCR. As the microarray allows the determination of the expression profile of many ABC transporters in a single hybridization experiment, it may be useful as a diagnostic tool to detect drug resistance in clinical samples.</jats:p>
Autoren
  • Jean-Pierre Gillet
  • Thomas Efferth
  • Daniel Steinbach
  • Jacques Hamels
  • Françoise de Longueville
  • Vincent Bertholet
  • José Remacle
DOI
10.1158/0008-5472.can-04-1978
eISSN
1538-7445
ISSN
0008-5472
Ausgabe der Veröffentlichung
24
Zeitschrift
Cancer Research
Sprache
en
Online publication date
2004
Paginierung
8987 - 8993
Datum der Veröffentlichung
2004
Status
Published
Herausgeber
American Association for Cancer Research (AACR)
Herausgeber URL
http://dx.doi.org/10.1158/0008-5472.can-04-1978
Datum der Datenerfassung
2022
Titel
Microarray-based Detection of Multidrug Resistance in Human Tumor Cells by Expression Profiling of ATP-binding Cassette Transporter Genes
Ausgabe der Zeitschrift
64

Data source: Crossref

Abstract
Different mechanisms of drug resistance, including ATP-binding cassette (ABC) transporters, are responsible for treatment failure of tumors. We developed a low-density DNA microarray which contains 38 genes of the ABC transporter gene family. This tool has been validated with three different multidrug-resistant sublines (CEM/ADR5000, HL60/AR, and MCF7/CH1000) known to overexpress either the ABCB1 (MDR1), ABCC1 (MRP1), or ABCG2 (MXR and BCRP) genes. When compared with their drug-sensitive parental lines, we observed not only the overexpression of these genes in the multidrug-resistant cell lines but also of other ABC transporter genes pointing to their possible role in multidrug resistance. These results were corroborated by quantitative real-time reverse transcription-PCR. As the microarray allows the determination of the expression profile of many ABC transporters in a single hybridization experiment, it may be useful as a diagnostic tool to detect drug resistance in clinical samples.
Addresses
  • Research Unit of Cellular Biology, University of Namur, Namur, Belgium. jpierre.gillet@fundp.ac.be
Autoren
  • Jean-Pierre Gillet
  • Thomas Efferth
  • Thomas Efferth
  • Daniel Steinbach
  • Jacques Hamels
  • Françoise de Longueville
  • Vincent Bertholet
  • José Remacle
DOI
10.1158/0008-5472.can-04-1978
eISSN
1538-7445
Externe Identifier
  • PubMed Identifier: 15604263
Open access
false
ISSN
0008-5472
Ausgabe der Veröffentlichung
24
Zeitschrift
Cancer research
Schlüsselwörter
  • Cell Line, Tumor
  • HL-60 Cells
  • Humans
  • Leukemia, T-Cell
  • Breast Neoplasms
  • ATP-Binding Cassette Transporters
  • Oligonucleotide Array Sequence Analysis
  • Reproducibility of Results
  • Gene Expression Profiling
  • Reverse Transcriptase Polymerase Chain Reaction
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
Sprache
eng
Medium
Print
Paginierung
8987 - 8993
Datum der Veröffentlichung
2004
Status
Published
Datum der Datenerfassung
2004
Titel
Microarray-based detection of multidrug resistance in human tumor cells by expression profiling of ATP-binding cassette transporter genes.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
64

Data source: Europe PubMed Central

Abstract
Different mechanisms of drug resistance, including ATP-binding cassette (ABC) transporters, are responsible for treatment failure of tumors. We developed a low-density DNA microarray which contains 38 genes of the ABC transporter gene family. This tool has been validated with three different multidrug-resistant sublines (CEM/ADR5000, HL60/AR, and MCF7/CH1000) known to overexpress either the ABCB1 (MDR1), ABCC1 (MRP1), or ABCG2 (MXR and BCRP) genes. When compared with their drug-sensitive parental lines, we observed not only the overexpression of these genes in the multidrug-resistant cell lines but also of other ABC transporter genes pointing to their possible role in multidrug resistance. These results were corroborated by quantitative real-time reverse transcription-PCR. As the microarray allows the determination of the expression profile of many ABC transporters in a single hybridization experiment, it may be useful as a diagnostic tool to detect drug resistance in clinical samples.
Autoren
  • Jean-Pierre Gillet
  • Thomas Efferth
  • Daniel Steinbach
  • Jacques Hamels
  • Françoise de Longueville
  • Vincent Bertholet
  • José Remacle
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/15604263
DOI
10.1158/0008-5472.CAN-04-1978
ISSN
0008-5472
Ausgabe der Veröffentlichung
24
Zeitschrift
Cancer Res
Schlüsselwörter
  • ATP-Binding Cassette Transporters
  • Breast Neoplasms
  • Cell Line, Tumor
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Gene Expression Profiling
  • HL-60 Cells
  • Humans
  • Leukemia, T-Cell
  • Oligonucleotide Array Sequence Analysis
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction
Sprache
eng
Country
United States
Paginierung
8987 - 8993
PII
64/24/8987
Datum der Veröffentlichung
2004
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2005
Titel
Microarray-based detection of multidrug resistance in human tumor cells by expression profiling of ATP-binding cassette transporter genes.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
64

Data source: PubMed

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