Inhibition of angiogenesis in vivo and growth of Kaposi's sarcoma xenograft tumors by the anti-malarial artesunate

Autoren

Raffaella Dell’Eva
Ulrich Pfeffer
Roberta Vené
Luca Anfosso
Alessandra Forlani
Adriana Albini
Thomas Efferth

DOI

10.1016/j.bcp.2004.08.021

ISSN

0006-2952

Ausgabe der Veröffentlichung

12

Zeitschrift

Biochemical Pharmacology

Sprache

en

Paginierung

2359 - 2366

Datum der Veröffentlichung

2004

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.bcp.2004.08.021

Datum der Datenerfassung

2020

Titel

Inhibition of angiogenesis in vivo and growth of Kaposi's sarcoma xenograft tumors by the anti-malarial artesunate

Ausgabe der Zeitschrift

68

Data source: Crossref

Abstract

Artesunate (ART) is a semi-synthetic derivative of the sesquiterpene artemisinin used for the second line therapy of malaria infections with Plasmodium falciparum. ART also inhibits growth of many transformed cell lines. In the present investigation, we show that ART inhibited the growth of normal human umbilical endothelial cells and of KS-IMM cells that we have established from a Kaposi's sarcoma lesion obtained from a renal transplant patient. The growth inhibitory activity correlated with the induction of apoptosis in KS-IMM cells. Apoptosis was not observed in normal endothelial cells, which, however, showed drastically increased cell doubling times upon ART treatment. ART strongly reduced angiogenesis in vivo in terms of vascularization of Matrigel plugs injected subcutaneously into syngenic mice. We conclude that ART represents a promising candidate drug for the treatment of the highly angiogenic Kaposi's sarcoma. As a low-cost drug, it might be of particular interest for areas of Kaposi's sarcoma endemics. ART could be useful for the prevention of tumor angiogenesis.

Addresses

Molecular Oncology, National Cancer Research Institute (IST), Largo Rosanna Benzi 10, 16132 Genova, Italy.

Autoren

Raffaella Dell'Eva
Ulrich Pfeffer
Roberta Vené
Luca Anfosso
Alessandra Forlani
Adriana Albini
Thomas Efferth
Thomas Efferth

DOI

10.1016/j.bcp.2004.08.021

eISSN

1873-2968

Externe Identifier

PubMed Identifier: 15548382

Open access

false

ISSN

0006-2952

Ausgabe der Veröffentlichung

12

Zeitschrift

Biochemical pharmacology

Schlüsselwörter

Animals
Mice, Inbred C57BL
Mice
Mice, Nude
Sarcoma, Kaposi
Disease Models, Animal
Sesquiterpenes
Artemisinins
Collagen
Metalloproteases
Proteoglycans
Angiogenesis Inhibitors
Laminin
Antineoplastic Agents
Drug Combinations
Antimalarials
Xenograft Model Antitumor Assays
Neoplasm Transplantation
Apoptosis
Neovascularization, Physiologic
Male
Artesunate

Sprache

eng

Medium

Print

Paginierung

2359 - 2366

Datum der Veröffentlichung

2004

Status

Published

Datum der Datenerfassung

2004

Titel

Inhibition of angiogenesis in vivo and growth of Kaposi's sarcoma xenograft tumors by the anti-malarial artesunate.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

68

Data source: Europe PubMed Central

Abstract

Artesunate (ART) is a semi-synthetic derivative of the sesquiterpene artemisinin used for the second line therapy of malaria infections with Plasmodium falciparum. ART also inhibits growth of many transformed cell lines. In the present investigation, we show that ART inhibited the growth of normal human umbilical endothelial cells and of KS-IMM cells that we have established from a Kaposi's sarcoma lesion obtained from a renal transplant patient. The growth inhibitory activity correlated with the induction of apoptosis in KS-IMM cells. Apoptosis was not observed in normal endothelial cells, which, however, showed drastically increased cell doubling times upon ART treatment. ART strongly reduced angiogenesis in vivo in terms of vascularization of Matrigel plugs injected subcutaneously into syngenic mice. We conclude that ART represents a promising candidate drug for the treatment of the highly angiogenic Kaposi's sarcoma. As a low-cost drug, it might be of particular interest for areas of Kaposi's sarcoma endemics. ART could be useful for the prevention of tumor angiogenesis.

Date of acceptance

2004

Autoren

Raffaella Dell'Eva
Ulrich Pfeffer
Roberta Vené
Luca Anfosso
Alessandra Forlani
Adriana Albini
Thomas Efferth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/15548382

DOI

10.1016/j.bcp.2004.08.021

ISSN

0006-2952

Ausgabe der Veröffentlichung

12

Zeitschrift

Biochem Pharmacol

Schlüsselwörter

Angiogenesis Inhibitors
Animals
Antimalarials
Antineoplastic Agents
Apoptosis
Artemisinins
Artesunate
Collagen
Disease Models, Animal
Drug Combinations
Laminin
Male
Metalloproteases
Mice
Mice, Inbred C57BL
Mice, Nude
Neoplasm Transplantation
Neovascularization, Physiologic
Proteoglycans
Sarcoma, Kaposi
Sesquiterpenes
Xenograft Model Antitumor Assays

Sprache

eng

Country

England

Paginierung

2359 - 2366

PII

S0006-2952(04)00599-4

Datum der Veröffentlichung

2004

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2005

Titel

Inhibition of angiogenesis in vivo and growth of Kaposi's sarcoma xenograft tumors by the anti-malarial artesunate.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

68

Data source: PubMed

Inhibition of angiogenesis in vivo and growth of Kaposi's sarcoma xenograft tumors by the anti-malarial artesunate

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