Microarray-based prediction of cytotoxicity of tumor cells to cantharidin

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000227097800013&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 898SZ
  • PubMed Identifier: 15706417
ISSN
1021-335X
Ausgabe der Veröffentlichung
3
Zeitschrift
ONCOLOGY REPORTS
Schlüsselwörter
  • chemotherapy
  • drug resistance
  • hierarchical cluster analysis
  • microarrays
Paginierung
459 - 463
Datum der Veröffentlichung
2005
Status
Published
Titel
Microarray-based prediction of cytotoxicity of tumor cells to cantharidin
Sub types
  • Article
Ausgabe der Zeitschrift
13

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Cantharidin (CAN) is the active principle of the Chinese blister beetle (Mylabris phalerata) which exerts profound cytotoxicity towards tumor cells. The aim of this study was to identify the molecular determinants of sensitivity and resistance of tumor cells to CAN. We mined the microarray database of the National Cancer Institute (NCI), for genes whose expression correlated with the IC(50) values for CAN of 60 cell lines of different tumor types. By COMPARE analysis Kendall's tau test, and false discovery rate (FDR) analysis, 21 out of 9706 genes or expressed sequence tags (ESTs) were identified. If the mRNA expression of the 21 genes or ESTs was subjected to hierarchical cluster analysis and cluster image mapping, sensitivity or resistance of the 60 cell lines to CAN was predictable with statistical significance. The majority of these genes are involved in DNA damage response, DNA repair, and/or apoptosis. In conclusion, sensitivity or resistance of tumor cells to CAN is multi-factorial in nature. DNA repair and apoptosis play a major role as determinants of cellular response to CAN. The present investigation represents a starting point to dissect the genes and molecular pathways responsible for cellular response to cantharidin in more detail.
Addresses
  • German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany. thomas.efferth@web.de
Autoren
eISSN
1791-2431
Externe Identifier
  • PubMed Identifier: 15706417
Open access
false
ISSN
1021-335X
Ausgabe der Veröffentlichung
3
Zeitschrift
Oncology reports
Schlüsselwörter
  • Tumor Cells, Cultured
  • Humans
  • DNA Damage
  • Cantharidin
  • RNA, Messenger
  • Enzyme Inhibitors
  • Oligonucleotide Array Sequence Analysis
  • Drug Screening Assays, Antitumor
  • Cluster Analysis
  • Predictive Value of Tests
  • Gene Expression Profiling
  • Apoptosis
  • DNA Repair
  • Drug Resistance, Neoplasm
  • Databases, Genetic
Sprache
eng
Medium
Print
Paginierung
459 - 463
Datum der Veröffentlichung
2005
Status
Published
Datum der Datenerfassung
2005
Titel
Microarray-based prediction of cytotoxicity of tumor cells to cantharidin.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
13

Data source: Europe PubMed Central

Abstract
Cantharidin (CAN) is the active principle of the Chinese blister beetle (Mylabris phalerata) which exerts profound cytotoxicity towards tumor cells. The aim of this study was to identify the molecular determinants of sensitivity and resistance of tumor cells to CAN. We mined the microarray database of the National Cancer Institute (NCI), for genes whose expression correlated with the IC(50) values for CAN of 60 cell lines of different tumor types. By COMPARE analysis Kendall's tau test, and false discovery rate (FDR) analysis, 21 out of 9706 genes or expressed sequence tags (ESTs) were identified. If the mRNA expression of the 21 genes or ESTs was subjected to hierarchical cluster analysis and cluster image mapping, sensitivity or resistance of the 60 cell lines to CAN was predictable with statistical significance. The majority of these genes are involved in DNA damage response, DNA repair, and/or apoptosis. In conclusion, sensitivity or resistance of tumor cells to CAN is multi-factorial in nature. DNA repair and apoptosis play a major role as determinants of cellular response to CAN. The present investigation represents a starting point to dissect the genes and molecular pathways responsible for cellular response to cantharidin in more detail.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/15706417
ISSN
1021-335X
Ausgabe der Veröffentlichung
3
Zeitschrift
Oncol Rep
Schlüsselwörter
  • Apoptosis
  • Cantharidin
  • Cluster Analysis
  • DNA Damage
  • DNA Repair
  • Databases, Genetic
  • Drug Resistance, Neoplasm
  • Drug Screening Assays, Antitumor
  • Enzyme Inhibitors
  • Gene Expression Profiling
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • Predictive Value of Tests
  • RNA, Messenger
  • Tumor Cells, Cultured
Sprache
eng
Country
Greece
Paginierung
459 - 463
Datum der Veröffentlichung
2005
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2005
Titel
Microarray-based prediction of cytotoxicity of tumor cells to cantharidin.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
13

Data source: PubMed

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