Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas

Publication type:
Journal article
Metadata:
Autoren
  • E Gebhart
  • J Ries
  • J Wiltfang
  • T Liehr
  • T Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000226505400003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.arcmed.2004.06.001
eISSN
1873-5487
Externe Identifier
  • Clarivate Analytics Document Solution ID: 890IR
  • PubMed Identifier: 15610907
ISSN
0188-4409
Ausgabe der Veröffentlichung
5
Zeitschrift
ARCHIVES OF MEDICAL RESEARCH
Schlüsselwörter
  • CGH
  • EGFR
  • complex genomic imbalances
  • oral SCC
Paginierung
385 - 394
Datum der Veröffentlichung
2004
Status
Published
Titel
Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas
Sub types
  • Article
Ausgabe der Zeitschrift
35

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Erich Gebhart
  • Jutta Ries
  • Jörg Wiltfang
  • Thomas Liehr
  • Thomas Efferth
DOI
10.1016/j.arcmed.2004.06.001
ISSN
0188-4409
Ausgabe der Veröffentlichung
5
Zeitschrift
Archives of Medical Research
Sprache
en
Paginierung
385 - 394
Datum der Veröffentlichung
2004
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.arcmed.2004.06.001
Datum der Datenerfassung
2024
Titel
Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas
Ausgabe der Zeitschrift
35

Data source: Crossref

Abstract
<h4>Background</h4>To determine the association between changes of genomic gene dose and clinical parameters in squamous cell carcinomas of the oral cavity, comparative genomic hybridization seemed suited not only to detect genomic imbalances in these tumors, but also particularly to examine the role of gain of 7p12, the band harboring the epidermal growth factor receptor (EGFR) in this context.<h4>Methods</h4>Total genomic DNA obtained from 35 oral squamous cell carcinomas was subjected to comparative genomic hybridization (CGH) and detected patterns of genomic imbalances were associated with various clinical parameters.<h4>Results</h4>The examined tumors exhibited five and up to 47 DNA copy number alterations (CNAs). Nineteen of these showed a gain of chromosome band 7p12. A highly complex but strikingly consistent pattern of genomic imbalances (average, 32 CNAs per tumor) was associated with this alteration, among which gains clearly dominated over losses of genomic material. Comparable patterns, however, could also be found in a few tumors with a high number of CNAs (average, 26) but without the 7p gain. Low numbers of imbalances always were accompanied by low consistency of CNA patterns and none of these cases showed enh(7p12). No significant differences with respect to pT class or grade of tumors were found between enh(7p)-positive and -negative tumors. Stage IV and lymph node affection were slightly more frequent among enh(7p12)-positive than in -negative cases. Relapse occurred in 63% in 7p12-positive vs. 25% in the negative group. Average disease-free survival of tumors without 7p gain clearly exceeded that of tumors with gain of 7p (36.8 vs. 21.3). However, some of these associations could also be found if comparison was based on number of CNAs. By means of hierarchical cluster analysis, we were able to show that different patterns of CNAs can be separated from each other in tumors with or without 7p alterations, and that these patterns predict short- or long-term survival of patients.<h4>Conclusions</h4>Previously described associations of gains of 7p12, the chromosomal band harboring the EGFR gene with clinical parameters can reasonably be estimated only within the context of the pattern and complexity of the genomic imbalances accompanying this chromosomal loss in examined tumors.
Addresses
  • Institute of Human Genetics, University of Erlangen-Nuremberg, Erlander, Germany. egebhart@humgenet.uni-erlangen.de
Autoren
  • Erich Gebhart
  • Jutta Ries
  • Jörg Wiltfang
  • Thomas Liehr
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.arcmed.2004.06.001
eISSN
1873-5487
Externe Identifier
  • PubMed Identifier: 15610907
Open access
false
ISSN
0188-4409
Ausgabe der Veröffentlichung
5
Zeitschrift
Archives of medical research
Schlüsselwörter
  • Chromosomes, Human, Pair 7
  • Humans
  • Carcinoma, Squamous Cell
  • Mouth Neoplasms
  • Cluster Analysis
  • Nucleic Acid Hybridization
  • Gene Dosage
  • Adult
  • Aged
  • Aged, 80 and over
  • Middle Aged
  • Female
  • Male
  • Statistics as Topic
  • Genetic Linkage
  • ErbB Receptors
Sprache
eng
Medium
Print
Paginierung
385 - 394
Datum der Veröffentlichung
2004
Status
Published
Datum der Datenerfassung
2004
Titel
Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas.
Sub types
  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
35

Data source: Europe PubMed Central

Abstract
BACKGROUND: To determine the association between changes of genomic gene dose and clinical parameters in squamous cell carcinomas of the oral cavity, comparative genomic hybridization seemed suited not only to detect genomic imbalances in these tumors, but also particularly to examine the role of gain of 7p12, the band harboring the epidermal growth factor receptor (EGFR) in this context. METHODS: Total genomic DNA obtained from 35 oral squamous cell carcinomas was subjected to comparative genomic hybridization (CGH) and detected patterns of genomic imbalances were associated with various clinical parameters. RESULTS: The examined tumors exhibited five and up to 47 DNA copy number alterations (CNAs). Nineteen of these showed a gain of chromosome band 7p12. A highly complex but strikingly consistent pattern of genomic imbalances (average, 32 CNAs per tumor) was associated with this alteration, among which gains clearly dominated over losses of genomic material. Comparable patterns, however, could also be found in a few tumors with a high number of CNAs (average, 26) but without the 7p gain. Low numbers of imbalances always were accompanied by low consistency of CNA patterns and none of these cases showed enh(7p12). No significant differences with respect to pT class or grade of tumors were found between enh(7p)-positive and -negative tumors. Stage IV and lymph node affection were slightly more frequent among enh(7p12)-positive than in -negative cases. Relapse occurred in 63% in 7p12-positive vs. 25% in the negative group. Average disease-free survival of tumors without 7p gain clearly exceeded that of tumors with gain of 7p (36.8 vs. 21.3). However, some of these associations could also be found if comparison was based on number of CNAs. By means of hierarchical cluster analysis, we were able to show that different patterns of CNAs can be separated from each other in tumors with or without 7p alterations, and that these patterns predict short- or long-term survival of patients. CONCLUSIONS: Previously described associations of gains of 7p12, the chromosomal band harboring the EGFR gene with clinical parameters can reasonably be estimated only within the context of the pattern and complexity of the genomic imbalances accompanying this chromosomal loss in examined tumors.
Date of acceptance
2004
Autoren
  • Erich Gebhart
  • Jutta Ries
  • Jörg Wiltfang
  • Thomas Liehr
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/15610907
DOI
10.1016/j.arcmed.2004.06.001
ISSN
0188-4409
Ausgabe der Veröffentlichung
5
Zeitschrift
Arch Med Res
Schlüsselwörter
  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Squamous Cell
  • Chromosomes, Human, Pair 7
  • Cluster Analysis
  • ErbB Receptors
  • Female
  • Gene Dosage
  • Genetic Linkage
  • Humans
  • Male
  • Middle Aged
  • Mouth Neoplasms
  • Nucleic Acid Hybridization
  • Statistics as Topic
Sprache
eng
Country
United States
Paginierung
385 - 394
PII
S0188-4409(04)00084-0
Datum der Veröffentlichung
2004
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2005
Titel
Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas.
Sub types
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
35

Data source: PubMed

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