FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM

Publication type:
Journal article
Metadata:
Autoren
  • U Wick
  • M Kirsch
  • A Rauch
  • I Chudoba
  • B Lausen
  • T Efferth
  • E Gebhart
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000231728300005&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1159/000085667
eISSN
1424-859X
Externe Identifier
  • Clarivate Analytics Document Solution ID: 962JS
  • PubMed Identifier: 16093718
ISSN
1424-8581
Ausgabe der Veröffentlichung
1
Zeitschrift
CYTOGENETIC AND GENOME RESEARCH
Paginierung
34 - 40
Datum der Veröffentlichung
2005
Status
Published
Titel
FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM
Sub types
  • Article
Ausgabe der Zeitschrift
111

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:p>So far, the problem of an influence of translocations on the telomeres of the involved chromosomes has not been addressed yet in human cells. Therefore, the telomeres of a karyotypically rather well characterized T-cell acute lymphoblastic leukemia (T-ALL) cell line (CCRF-CEM) with several marker chromosomes were examined using peptide nucleic acid (PNA) telomere FISH probes to compare the telomere length of these markers with that of the chromosome arms of their origin. In addition, chromosome libraries, centromeric probes, and subtelomeric DNA probes were used to further define the marker chromosomes. Two markers could be newly defined and a concise karyotype of the cell line could be obtained by these detailed examinations: 42–47,X,–X,del(5) (q35?),t(5;15)(q14;q13.2),t(8;9)(p11;p24),del(9)(:p13→qter)/inv(9)(pter→p12::q21→p12::q21→qter),+13,+20,+der(22)(p+ [HSR?])[cp]. The relative telomere length of all chromosomes showed considerable interchromosomal, intercellular, and inter-passage variation. However, it could be shown, that in four different passages of the examined cell line the observed differences between relative telomere lengths of the markers and the chromosomes of their origin, with two exceptions (short arms of del/inv9 and der22), were not significant. On the other hand, because of its mentioned variability, telomere length alone is not sufficient to reliably define the derivation of markers.   </jats:p>
Autoren
  • U Wick
  • M Kirsch
  • A Rauch
  • I Chudoba
  • B Lausen
  • T Efferth
  • E Gebhart
DOI
10.1159/000085667
eISSN
1424-859X
ISSN
1424-8581
Ausgabe der Veröffentlichung
1
Zeitschrift
Cytogenetic and Genome Research
Sprache
en
Online publication date
2005
Paginierung
34 - 40
Datum der Veröffentlichung
2005
Status
Published
Herausgeber
S. Karger AG
Herausgeber URL
http://dx.doi.org/10.1159/000085667
Datum der Datenerfassung
2022
Titel
FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM
Ausgabe der Zeitschrift
111

Data source: Crossref

Abstract
So far, the problem of an influence of translocations on the telomeres of the involved chromosomes has not been addressed yet in human cells. Therefore, the telomeres of a karyotypically rather well characterized T-cell acute lymphoblastic leukemia (T-ALL) cell line (CCRF-CEM) with several marker chromosomes were examined using peptide nucleic acid (PNA) telomere FISH probes to compare the telomere length of these markers with that of the chromosome arms of their origin. In addition, chromosome libraries, centromeric probes, and subtelomeric DNA probes were used to further define the marker chromosomes. Two markers could be newly defined and a concise karyotype of the cell line could be obtained by these detailed examinations: 42-47,X,-X,del(5) (q35?),t(5;15)(q14;q13.2),t(8;9)(p11;p24),del(9)(:p13-->qter)/inv(9)(pter-->p12::q21-->p12::q21-->qter),+13,+20,+der(22)(p+ [HSR?])[cp]. The relative telomere length of all chromosomes showed considerable interchromosomal, intercellular, and inter-passage variation. However, it could be shown, that in four different passages of the examined cell line the observed differences between relative telomere lengths of the markers and the chromosomes of their origin, with two exceptions (short arms of del/inv9 and der22), were not significant. On the other hand, because of its mentioned variability, telomere length alone is not sufficient to reliably define the derivation of markers.
Addresses
  • Institute of Human Genetics, University of Erlangen-Nürnberg, Erlangen, Germany.
Autoren
  • U Wick
  • M Kirsch
  • A Rauch
  • I Chudoba
  • B Lausen
  • T Efferth
  • T Efferth
  • E Gebhart
DOI
10.1159/000085667
eISSN
1424-859X
Externe Identifier
  • PubMed Identifier: 16093718
Open access
false
ISSN
1424-8581
Ausgabe der Veröffentlichung
1
Zeitschrift
Cytogenetic and genome research
Schlüsselwörter
  • Cell Line, Tumor
  • Telomere
  • Humans
  • Chromosome Aberrations
  • Genetic Markers
  • In Situ Hybridization, Fluorescence
  • Chromosome Mapping
  • Karyotyping
  • Leukemia-Lymphoma, Adult T-Cell
Sprache
eng
Medium
Print
Paginierung
34 - 40
Datum der Veröffentlichung
2005
Status
Published
Datum der Datenerfassung
2005
Titel
FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
111

Data source: Europe PubMed Central

Abstract
So far, the problem of an influence of translocations on the telomeres of the involved chromosomes has not been addressed yet in human cells. Therefore, the telomeres of a karyotypically rather well characterized T-cell acute lymphoblastic leukemia (T-ALL) cell line (CCRF-CEM) with several marker chromosomes were examined using peptide nucleic acid (PNA) telomere FISH probes to compare the telomere length of these markers with that of the chromosome arms of their origin. In addition, chromosome libraries, centromeric probes, and subtelomeric DNA probes were used to further define the marker chromosomes. Two markers could be newly defined and a concise karyotype of the cell line could be obtained by these detailed examinations: 42-47,X,-X,del(5) (q35?),t(5;15)(q14;q13.2),t(8;9)(p11;p24),del(9)(:p13-->qter)/inv(9)(pter-->p12::q21-->p12::q21-->qter),+13,+20,+der(22)(p+ [HSR?])[cp]. The relative telomere length of all chromosomes showed considerable interchromosomal, intercellular, and inter-passage variation. However, it could be shown, that in four different passages of the examined cell line the observed differences between relative telomere lengths of the markers and the chromosomes of their origin, with two exceptions (short arms of del/inv9 and der22), were not significant. On the other hand, because of its mentioned variability, telomere length alone is not sufficient to reliably define the derivation of markers.
Date of acceptance
2004
Autoren
  • U Wick
  • M Kirsch
  • A Rauch
  • I Chudoba
  • B Lausen
  • T Efferth
  • E Gebhart
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/16093718
DOI
10.1159/000085667
eISSN
1424-859X
Ausgabe der Veröffentlichung
1
Zeitschrift
Cytogenet Genome Res
Schlüsselwörter
  • Cell Line, Tumor
  • Chromosome Aberrations
  • Chromosome Mapping
  • Genetic Markers
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Leukemia-Lymphoma, Adult T-Cell
  • Telomere
Sprache
eng
Country
Switzerland
Paginierung
34 - 40
PII
85667
Datum der Veröffentlichung
2005
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2005
Titel
FISH studies on the telomeric regions of the T-cell acute lymphoblastic leukemia cell line CCRF-CEM.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
111

Data source: PubMed

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