Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an "in vitro" replicative system

Autoren

Marta R Romero
Thomas Efferth
Maria A Serrano
Beatriz Castaño
Rocio IR Macias
Oscar Briz
Jose JG Marin

DOI

10.1016/j.antiviral.2005.07.005

ISSN

0166-3542

Ausgabe der Veröffentlichung

2

Zeitschrift

Antiviral Research

Sprache

en

Paginierung

75 - 83

Datum der Veröffentlichung

2005

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.antiviral.2005.07.005

Datum der Datenerfassung

2020

Titel

Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an “in vitro” replicative system

Ausgabe der Zeitschrift

68

Data source: Crossref

Abstract

The antiviral effect against hepatitis B virus (HBV) of artemisinin, its derivative artesunate and other compounds highly purified from traditional Chinese medicine remedies, were investigated. HBV production by permanently transfected HepG2 2.2.15 cells was determined by measuring the release of surface protein (HBsAg) and HBV-DNA after drug exposure (0.01-100 microM) for 21 days. The forms of HBV-DNA released were investigated by Southern-blotting. Neutral Red retention test was used to evaluate drug-induced toxicity on host cells. The compounds were classified according to their potential interest as follows: (i) none: they had no effect on viral production (daidzein, daidzin, isonardosinon, nardofuran, nardosinon, tetrahydronardosinon and quercetin); (ii) low: they were able to markedly reduce viral production, but also induced toxicity on host cells (berberine and tannic acid) or they had no toxic effect on host cells but only had a moderate ability to reduce viral production (curcumin, baicalein, baicalin, bufalin, diallyl disulphide, glycyrrhizic acid and puerarin); (iii) high: they induced strong inhibition of viral production at concentrations at which host cell viability was not affected (artemisinin and artesunate). Moreover, artesunate in conjunction with lamivudine had synergic anti-HBV effects, which warrants further evaluation of artemisinin/artesunate as antiviral agents against HBV infection.

Addresses

Department of Biochemistry and Molecular Biology, University of Salamanca, Spain.

Autoren

Marta R Romero
Thomas Efferth
Thomas Efferth
Maria A Serrano
Beatriz Castaño
Rocio IR Macias
Oscar Briz
Jose JG Marin

DOI

10.1016/j.antiviral.2005.07.005

eISSN

1872-9096

Externe Identifier

PubMed Identifier: 16122816

Open access

false

ISSN

0166-3542

Ausgabe der Veröffentlichung

2

Zeitschrift

Antiviral research

Schlüsselwörter

Cell Line
Humans
Hepatitis B virus
Sesquiterpenes
Artemisinins
DNA, Viral
Lamivudine
Plant Extracts
Hepatitis B Surface Antigens
Antiviral Agents
Anti-HIV Agents
Culture Media
Indicators and Reagents
Virus Replication
Artesunate

Sprache

eng

Medium

Print-Electronic

Online publication date

2005

Paginierung

75 - 83

Datum der Veröffentlichung

2005

Status

Published

Datum der Datenerfassung

2005

Titel

Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an "in vitro" replicative system.

Sub types

Comparative Study
Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

68

Data source: Europe PubMed Central

Abstract

The antiviral effect against hepatitis B virus (HBV) of artemisinin, its derivative artesunate and other compounds highly purified from traditional Chinese medicine remedies, were investigated. HBV production by permanently transfected HepG2 2.2.15 cells was determined by measuring the release of surface protein (HBsAg) and HBV-DNA after drug exposure (0.01-100 microM) for 21 days. The forms of HBV-DNA released were investigated by Southern-blotting. Neutral Red retention test was used to evaluate drug-induced toxicity on host cells. The compounds were classified according to their potential interest as follows: (i) none: they had no effect on viral production (daidzein, daidzin, isonardosinon, nardofuran, nardosinon, tetrahydronardosinon and quercetin); (ii) low: they were able to markedly reduce viral production, but also induced toxicity on host cells (berberine and tannic acid) or they had no toxic effect on host cells but only had a moderate ability to reduce viral production (curcumin, baicalein, baicalin, bufalin, diallyl disulphide, glycyrrhizic acid and puerarin); (iii) high: they induced strong inhibition of viral production at concentrations at which host cell viability was not affected (artemisinin and artesunate). Moreover, artesunate in conjunction with lamivudine had synergic anti-HBV effects, which warrants further evaluation of artemisinin/artesunate as antiviral agents against HBV infection.

Date of acceptance

2005

Autoren

Marta R Romero
Thomas Efferth
Maria A Serrano
Beatriz Castaño
Rocio IR Macias
Oscar Briz
Jose JG Marin

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/16122816

DOI

10.1016/j.antiviral.2005.07.005

ISSN

0166-3542

Ausgabe der Veröffentlichung

2

Zeitschrift

Antiviral Res

Schlüsselwörter

Anti-HIV Agents
Antiviral Agents
Artemisinins
Artesunate
Cell Line
Culture Media
DNA, Viral
Hepatitis B Surface Antigens
Hepatitis B virus
Humans
Indicators and Reagents
Lamivudine
Plant Extracts
Sesquiterpenes
Virus Replication

Sprache

eng

Country

Netherlands

Paginierung

75 - 83

PII

S0166-3542(05)00147-6

Datum der Veröffentlichung

2005

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2006

Titel

Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an "in vitro" replicative system.

Sub types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

68

Data source: PubMed

Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an "in vitro" replicative system

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