Microarray expression profiles of angiogenesis-related genes predict tumor cell response to artemisinins

Autoren

L Anfosso
T Efferth
A Albini
U Pfeffer

DOI

10.1038/sj.tpj.6500371

eISSN

1473-1150

ISSN

1470-269X

Ausgabe der Veröffentlichung

4

Zeitschrift

The Pharmacogenomics Journal

Sprache

en

Online publication date

2006

Paginierung

269 - 278

Datum der Veröffentlichung

2006

Status

Published

Herausgeber

Springer Science and Business Media LLC

Herausgeber URL

http://dx.doi.org/10.1038/sj.tpj.6500371

Datum der Datenerfassung

2022

Titel

Microarray expression profiles of angiogenesis-related genes predict tumor cell response to artemisinins

Ausgabe der Zeitschrift

6

Data source: Crossref

Abstract

Artemisinin (ARS) and its derivatives are used for the second-line therapy of malaria infections with Plasmodium falciparum and P. vivax. ARSs also reveal profound antitumor activity in vitro and in vivo. In the present investigation, we correlated the mRNA expression data of 89 angiogenesis-related genes obtained by microarray hybridization from the database of the US National Cancer Institute with the 50% growth inhibition concentration values for eight ARSs (ARS, arteether (ARE), artesunate (ART), artemisetene, arteanuine B, dihydroartemisinylester stereoisomers 1 and 2). The constitutive expression of 30 genes correlated significantly with the cellular response to ARSs. By means of hierarchical cluster analysis and cluster image mapping expression, profiles were identified that determined significantly the cellular response to ART, ARE, artemether and dihydroartemisinylester stereoisomer 1. We have exemplarily validated the microarray data of six out of these 30 genes by real-time RT-PCR in seven cell lines. The fact that sensitivity and resistance of tumor cells could be predicted by the mRNA expression of angiogenesis-related genes indicate that ARSs reveal their antitumor effects at least in part by inhibition of tumor angiogenesis. As many chemopreventive drugs exert antiangiogenic features, ARSs might also be chemopreventive in addition to their cytotoxic effects.

Addresses

Experimental Oncology A, National Cancer Research Institute, Genova, Italy.

Autoren

L Anfosso
T Efferth
T Efferth
A Albini
U Pfeffer

DOI

10.1038/sj.tpj.6500371

eISSN

1473-1150

Externe Identifier

PubMed Identifier: 16432535

Open access

false

ISSN

1470-269X

Ausgabe der Veröffentlichung

4

Zeitschrift

The pharmacogenomics journal

Schlüsselwörter

Cell Line, Tumor
Humans
Neoplasms
Neovascularization, Pathologic
Artemisinins
Angiogenic Proteins
RNA, Messenger
Antineoplastic Agents
Oligonucleotide Array Sequence Analysis
Cluster Analysis
Reproducibility of Results
Gene Expression Profiling
Reverse Transcriptase Polymerase Chain Reaction
Inhibitory Concentration 50
Cell Survival
Gene Expression Regulation

Sprache

eng

Medium

Print-Electronic

Online publication date

2006

Paginierung

269 - 278

Datum der Veröffentlichung

2006

Status

Published

Datum der Datenerfassung

2006

Titel

Microarray expression profiles of angiogenesis-related genes predict tumor cell response to artemisinins.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

6

Data source: Europe PubMed Central

Abstract

Artemisinin (ARS) and its derivatives are used for the second-line therapy of malaria infections with Plasmodium falciparum and P. vivax. ARSs also reveal profound antitumor activity in vitro and in vivo. In the present investigation, we correlated the mRNA expression data of 89 angiogenesis-related genes obtained by microarray hybridization from the database of the US National Cancer Institute with the 50% growth inhibition concentration values for eight ARSs (ARS, arteether (ARE), artesunate (ART), artemisetene, arteanuine B, dihydroartemisinylester stereoisomers 1 and 2). The constitutive expression of 30 genes correlated significantly with the cellular response to ARSs. By means of hierarchical cluster analysis and cluster image mapping expression, profiles were identified that determined significantly the cellular response to ART, ARE, artemether and dihydroartemisinylester stereoisomer 1. We have exemplarily validated the microarray data of six out of these 30 genes by real-time RT-PCR in seven cell lines. The fact that sensitivity and resistance of tumor cells could be predicted by the mRNA expression of angiogenesis-related genes indicate that ARSs reveal their antitumor effects at least in part by inhibition of tumor angiogenesis. As many chemopreventive drugs exert antiangiogenic features, ARSs might also be chemopreventive in addition to their cytotoxic effects.

Autoren

L Anfosso
T Efferth
A Albini
U Pfeffer

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/16432535

DOI

10.1038/sj.tpj.6500371

ISSN

1470-269X

Ausgabe der Veröffentlichung

4

Zeitschrift

Pharmacogenomics J

Schlüsselwörter

Angiogenic Proteins
Antineoplastic Agents
Artemisinins
Cell Line, Tumor
Cell Survival
Cluster Analysis
Gene Expression Profiling
Gene Expression Regulation
Humans
Inhibitory Concentration 50
Neoplasms
Neovascularization, Pathologic
Oligonucleotide Array Sequence Analysis
RNA, Messenger
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction

Sprache

eng

Country

United States

Paginierung

269 - 278

PII

6500371

Datum der Veröffentlichung

2006

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2006

Titel

Microarray expression profiles of angiogenesis-related genes predict tumor cell response to artemisinins.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

6

Data source: PubMed

Microarray expression profiles of angiogenesis-related genes predict tumor cell response to artemisinins

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