Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV)

Autoren

Marta Romero
Maria Serrano
Marta Vallejo
Thomas Efferth
Marcelino Alvarez
Jose Marin

DOI

10.1055/s-2006-947198

eISSN

1439-0221

ISSN

0032-0943

Ausgabe der Veröffentlichung

13

Zeitschrift

Planta Medica

Sprache

en

Paginierung

1169 - 1174

Datum der Veröffentlichung

2006

Status

Published

Herausgeber

Georg Thieme Verlag KG

Herausgeber URL

http://dx.doi.org/10.1055/s-2006-947198

Datum der Datenerfassung

2017

Titel

Antiviral Effect of Artemisinin from <i>Artemisia annua</i> against a Model Member of the <i>Flaviviridae</i> Family, the Bovine Viral Diarrhoea Virus (BVDV)

Ausgabe der Zeitschrift

72

Data source: Crossref

Abstract

The antiviral activity versus flaviviruses of artemisinin, a safe drug obtained from Artemisia annua and commonly used to treat malaria, has been investigated using as an IN VITRO model bovine epithelial cells from embryonic trachea (EBTr) infected with the cytopathic strain Oregon C24V, of bovine viral diarrhoea virus (BVDV), which is a member of the Flaviviridae family. Antiviral activity was estimated by the degree of protection against the cytopathic effect of BVDV on host cells and by the reduction in BVDV-RNA release to the culture medium. To induce an intermediate cytopathic effect in non-treated cells, EBTr cells were first exposed to BVDV for 48 h and then incubated with virus-free medium for 72 h. Ribavirin and artemisinin (up to 100 microM) induced no toxicity in host cells, whereas a slight degree of toxicity was observed for IFN-alpha at concentrations above 10 U/mL up to 100 U/mL. Treatment of infected cells with IFN-alpha, ribavirin and artemisinin markedly reduced BVDV-induced cell death. A combination of these drugs resulted in an additive protective effect. These drugs induced a significant reduction in the production/release of BVDV virions by infected EBTr cells; there was also an additive effect when combinations of them were assayed. These results suggest a potential usefulness of artemisinin in combination with current pharmacological therapy for the treatment of human and veterinary infections by flaviviruses.

Addresses

Laboratory of Experimental Hepatology and Drug Targeting (HEVEFARM), University of Salamanca, Salamanca, Spain.

Autoren

Marta R Romero
Maria A Serrano
Marta Vallejo
Thomas Efferth
Thomas Efferth
Marcelino Alvarez
Jose JG Marin

DOI

10.1055/s-2006-947198

eISSN

1439-0221

Externe Identifier

PubMed Identifier: 16902856

Open access

false

ISSN

0032-0943

Ausgabe der Veröffentlichung

13

Zeitschrift

Planta medica

Schlüsselwörter

Cells, Cultured
Animals
Cattle
Diarrhea Viruses, Bovine Viral
Virion
Artemisia annua
Sesquiterpenes
Artemisinins
Interferon-alpha
Ribavirin
Antiviral Agents
Drug Synergism

Sprache

eng

Medium

Print-Electronic

Online publication date

2006

Paginierung

1169 - 1174

Datum der Veröffentlichung

2006

Status

Published

Datum der Datenerfassung

2006

Titel

Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV).

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

72

Data source: Europe PubMed Central

Abstract

The antiviral activity versus flaviviruses of artemisinin, a safe drug obtained from Artemisia annua and commonly used to treat malaria, has been investigated using as an IN VITRO model bovine epithelial cells from embryonic trachea (EBTr) infected with the cytopathic strain Oregon C24V, of bovine viral diarrhoea virus (BVDV), which is a member of the Flaviviridae family. Antiviral activity was estimated by the degree of protection against the cytopathic effect of BVDV on host cells and by the reduction in BVDV-RNA release to the culture medium. To induce an intermediate cytopathic effect in non-treated cells, EBTr cells were first exposed to BVDV for 48 h and then incubated with virus-free medium for 72 h. Ribavirin and artemisinin (up to 100 microM) induced no toxicity in host cells, whereas a slight degree of toxicity was observed for IFN-alpha at concentrations above 10 U/mL up to 100 U/mL. Treatment of infected cells with IFN-alpha, ribavirin and artemisinin markedly reduced BVDV-induced cell death. A combination of these drugs resulted in an additive protective effect. These drugs induced a significant reduction in the production/release of BVDV virions by infected EBTr cells; there was also an additive effect when combinations of them were assayed. These results suggest a potential usefulness of artemisinin in combination with current pharmacological therapy for the treatment of human and veterinary infections by flaviviruses.

Autoren

Marta R Romero
Maria A Serrano
Marta Vallejo
Thomas Efferth
Marcelino Alvarez
Jose JG Marin

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/16902856

DOI

10.1055/s-2006-947198

ISSN

0032-0943

Ausgabe der Veröffentlichung

13

Zeitschrift

Planta Med

Schlüsselwörter

Animals
Antiviral Agents
Artemisia annua
Artemisinins
Cattle
Cells, Cultured
Diarrhea Viruses, Bovine Viral
Drug Synergism
Interferon-alpha
Ribavirin
Sesquiterpenes
Virion

Sprache

eng

Country

Germany

Paginierung

1169 - 1174

Datum der Veröffentlichung

2006

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2007

Titel

Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV).

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

72

Data source: PubMed

Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV)

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