Optimization of luteolin separation from pigeonpea [Cajanus cajan (L.) Millsp.] leaves by macroporous resins

Autoren

Yujie Fu
Yuangang Zu
Wei Liu
Thomas Efferth
Naijing Zhang
Xiaona Liu
Yu Kong

DOI

10.1016/j.chroma.2006.08.067

ISSN

0021-9673

Ausgabe der Veröffentlichung

2

Zeitschrift

Journal of Chromatography A

Sprache

en

Paginierung

145 - 152

Datum der Veröffentlichung

2006

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.chroma.2006.08.067

Datum der Datenerfassung

2022

Titel

Optimization of luteolin separation from pigeonpea [Cajanus cajan (L.) Millsp.] leaves by macroporous resins

Ausgabe der Zeitschrift

1137

Data source: Crossref

Abstract

In the present study, the performance and separation characteristics of eight macroporous resins for the separation of luteolin (LU) from pigeonpea leaves extracts have been evaluated. The adsorption and desorption properties of LU on macroporous resins including AB-8, NKA-9, NKA-2, D3520, D101, H1020, H103 and AL-2 have been compared. AL-2 resin offers the best adsorption and desorption capacity for LU than other resins based on the research results, and its adsorption data at 25 degrees C fit best to the Freundlich isotherm. Dynamic adsorption and desorption experiments have been carried out with the column packed by AL-2 resin to optimize the separation process of LU from pigeonpea leaves extracts. The optimum parameters for adsorption were sample solution LU concentration 65.5 microg/ml, pH 5, processing volume 3 BV, flow rate 1.5BV/h, temperature 25 degrees C; for desorption were elution solvent ethanol-water (50:50, v/v) 2 BV and followed by ethanol-water (60:40, v/v) 2 BV, and flow rate 1BV/h. After treated with AL-2 resin, the LU content in the product was increased 19.8-fold from 0.129% to 2.55%, with a recovery yield of 78.54%. The results showed that AL-2 resin revealed a good ability to separate LU. Therefore, we conclude that results in this study may provide scientific references for the large-scale LU production from pigeonpea or other plants extracts.

Addresses

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.

Autoren

Yujie Fu
Yuangang Zu
Wei Liu
Thomas Efferth
Thomas Efferth
Naijing Zhang
Xiaona Liu
Yu Kong

DOI

10.1016/j.chroma.2006.08.067

eISSN

1873-3778

Externe Identifier

PubMed Identifier: 17126843

Open access

false

ISSN

0021-9673

Ausgabe der Veröffentlichung

2

Zeitschrift

Journal of chromatography. A

Schlüsselwörter

Cajanus
Plant Leaves
Luteolin
Resins, Synthetic
Plant Extracts
Chromatography, High Pressure Liquid
Adsorption
Hydrogen-Ion Concentration

Sprache

eng

Medium

Print-Electronic

Online publication date

2006

Paginierung

145 - 152

Datum der Veröffentlichung

2006

Status

Published

Datum der Datenerfassung

2006

Titel

Optimization of luteolin separation from pigeonpea [Cajanus cajan (L.) Millsp.] leaves by macroporous resins.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

1137

Data source: Europe PubMed Central

Abstract

In the present study, the performance and separation characteristics of eight macroporous resins for the separation of luteolin (LU) from pigeonpea leaves extracts have been evaluated. The adsorption and desorption properties of LU on macroporous resins including AB-8, NKA-9, NKA-2, D3520, D101, H1020, H103 and AL-2 have been compared. AL-2 resin offers the best adsorption and desorption capacity for LU than other resins based on the research results, and its adsorption data at 25 degrees C fit best to the Freundlich isotherm. Dynamic adsorption and desorption experiments have been carried out with the column packed by AL-2 resin to optimize the separation process of LU from pigeonpea leaves extracts. The optimum parameters for adsorption were sample solution LU concentration 65.5 microg/ml, pH 5, processing volume 3 BV, flow rate 1.5BV/h, temperature 25 degrees C; for desorption were elution solvent ethanol-water (50:50, v/v) 2 BV and followed by ethanol-water (60:40, v/v) 2 BV, and flow rate 1BV/h. After treated with AL-2 resin, the LU content in the product was increased 19.8-fold from 0.129% to 2.55%, with a recovery yield of 78.54%. The results showed that AL-2 resin revealed a good ability to separate LU. Therefore, we conclude that results in this study may provide scientific references for the large-scale LU production from pigeonpea or other plants extracts.

Date of acceptance

2006

Autoren

Yujie Fu
Yuangang Zu
Wei Liu
Thomas Efferth
Naijing Zhang
Xiaona Liu
Yu Kong

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/17126843

DOI

10.1016/j.chroma.2006.08.067

ISSN

0021-9673

Ausgabe der Veröffentlichung

2

Zeitschrift

J Chromatogr A

Schlüsselwörter

Adsorption
Cajanus
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Luteolin
Plant Extracts
Plant Leaves
Resins, Synthetic

Sprache

eng

Country

Netherlands

Paginierung

145 - 152

PII

S0021-9673(06)01637-2

Datum der Veröffentlichung

2006

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2007

Titel

Optimization of luteolin separation from pigeonpea [Cajanus cajan (L.) Millsp.] leaves by macroporous resins.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

1137

Data source: PubMed

Optimization of luteolin separation from pigeonpea [Cajanus cajan (L.) Millsp.] leaves by macroporous resins

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