A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo

Publication type:
Journal article
Metadata:
Autoren
  • Ananda Mookerjee
  • Jayati Mookerjee Basu
  • Surajit Majumder
  • Shilpak Chatterjee
  • Gouri S Panda
  • Pranabananda Dutta
  • Smarajit Pal
  • Pratima Mukherjee
  • Thomas Efferth
  • Syamal Roy
  • Soumitra K Choudhuri
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000242345100001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/1471-2407-6-267
Externe Identifier
  • Clarivate Analytics Document Solution ID: 109YI
  • PubMed Identifier: 17107616
ISSN
1471-2407
Zeitschrift
BMC CANCER
Artikelnummer
ARTN 267
Datum der Veröffentlichung
2006
Status
Published
Titel
A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo
Sub types
  • Article
Ausgabe der Zeitschrift
6

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>In search of a suitable GSH-depleting agent, a novel copper complex viz., copper <jats:italic>N</jats:italic>-(2-hydroxyacetophenone) glycinate (CuNG) has been synthesized, which was initially found to be a potential resistance modifying agent and later found to be an immunomodulator in mice model in different doses. The objective of the present work was to decipher the effect of CuNG on reactive oxygen species (ROS) generation and antioxidant enzymes in normal and doxorubicin-resistant Ehrlich ascites carcinoma (EAC/Dox)-bearing Swiss albino mice.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>The effect of CuNG has been studied on ROS generation, multidrug resistance-associated protein1 (MRP1) expression and on activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>CuNG increased ROS generation and reduced MRP1 expression in EAC/Dox cells while only temporarily depleted glutathione (GSH) within 2 h in heart, kidney, liver and lung of EAC/Dox bearing mice, which were restored within 24 h. The level of liver Cu was observed to be inversely proportional to the level of GSH. Moreover, CuNG modulated SOD, CAT and GPx in different organs and thereby reduced oxidative stress. Thus nontoxic dose of CuNG may be utilized to reduce MRP1 expression and thus sensitize EAC/Dox cells to standard chemotherapy. Moreover, CuNG modulated SOD, CAT and and GPx activities to reduce oxidative stress in some vital organs of EAC/Dox bearing mice. CuNG treatment also helped to recover liver and renal function in EAC/Dox bearing mice.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Based on our studies, we conclude that CuNG may be a promising candidate to sensitize drug resistant cancers in the clinic.</jats:p> </jats:sec>
Autoren
  • Ananda Mookerjee
  • Jayati Mookerjee Basu
  • Surajit Majumder
  • Shilpak Chatterjee
  • Gouri S Panda
  • Pranabananda Dutta
  • Smarajit Pal
  • Pratima Mukherjee
  • Thomas Efferth
  • Syamal Roy
  • Soumitra K Choudhuri
DOI
10.1186/1471-2407-6-267
eISSN
1471-2407
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC Cancer
Sprache
en
Artikelnummer
267
Online publication date
2006
Datum der Veröffentlichung
2006
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/1471-2407-6-267
Datum der Datenerfassung
2021
Titel
A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo
Ausgabe der Zeitschrift
6

Data source: Crossref

Abstract
<h4>Background</h4>In search of a suitable GSH-depleting agent, a novel copper complex viz., copper N-(2-hydroxyacetophenone) glycinate (CuNG) has been synthesized, which was initially found to be a potential resistance modifying agent and later found to be an immunomodulator in mice model in different doses. The objective of the present work was to decipher the effect of CuNG on reactive oxygen species (ROS) generation and antioxidant enzymes in normal and doxorubicin-resistant Ehrlich ascites carcinoma (EAC/Dox)-bearing Swiss albino mice.<h4>Methods</h4>The effect of CuNG has been studied on ROS generation, multidrug resistance-associated protein1 (MRP1) expression and on activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).<h4>Results</h4>CuNG increased ROS generation and reduced MRP1 expression in EAC/Dox cells while only temporarily depleted glutathione (GSH) within 2 h in heart, kidney, liver and lung of EAC/Dox bearing mice, which were restored within 24 h. The level of liver Cu was observed to be inversely proportional to the level of GSH. Moreover, CuNG modulated SOD, CAT and GPx in different organs and thereby reduced oxidative stress. Thus nontoxic dose of CuNG may be utilized to reduce MRP1 expression and thus sensitize EAC/Dox cells to standard chemotherapy. Moreover, CuNG modulated SOD, CAT and and GPx activities to reduce oxidative stress in some vital organs of EAC/Dox bearing mice. CuNG treatment also helped to recover liver and renal function in EAC/Dox bearing mice.<h4>Conclusion</h4>Based on our studies, we conclude that CuNG may be a promising candidate to sensitize drug resistant cancers in the clinic.
Addresses
  • Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute (CNCI), 37 S, P, Mukherjee Road, Calcutta-700026, India. anandamookerjee@yahoo.com <anandamookerjee@yahoo.com>
Autoren
  • Ananda Mookerjee
  • Jayati Mookerjee Basu
  • Surajit Majumder
  • Shilpak Chatterjee
  • Gouri S Panda
  • Pranabananda Dutta
  • Smarajit Pal
  • Pratima Mukherjee
  • Thomas Efferth
  • Thomas Efferth
  • Syamal Roy
  • Soumitra K Choudhuri
DOI
10.1186/1471-2407-6-267
eISSN
1471-2407
Externe Identifier
  • PubMed Identifier: 17107616
  • PubMed Central ID: PMC1660546
Open access
true
ISSN
1471-2407
Zeitschrift
BMC cancer
Schlüsselwörter
  • Animals
  • Mice
  • Carcinoma, Ehrlich Tumor
  • Reactive Oxygen Species
  • Organometallic Compounds
  • Doxorubicin
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glycine
  • Multidrug Resistance-Associated Proteins
  • Antibiotics, Antineoplastic
  • Drug Resistance, Neoplasm
Sprache
eng
Medium
Electronic
Online publication date
2006
Open access status
Open Access
Paginierung
267
Datum der Veröffentlichung
2006
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2006
Titel
A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
6

Files

https://bmccancer.biomedcentral.com/counter/pdf/10.1186/1471-2407-6-267 https://europepmc.org/articles/PMC1660546?pdf=render

Data source: Europe PubMed Central

Abstract
BACKGROUND: In search of a suitable GSH-depleting agent, a novel copper complex viz., copper N-(2-hydroxyacetophenone) glycinate (CuNG) has been synthesized, which was initially found to be a potential resistance modifying agent and later found to be an immunomodulator in mice model in different doses. The objective of the present work was to decipher the effect of CuNG on reactive oxygen species (ROS) generation and antioxidant enzymes in normal and doxorubicin-resistant Ehrlich ascites carcinoma (EAC/Dox)-bearing Swiss albino mice. METHODS: The effect of CuNG has been studied on ROS generation, multidrug resistance-associated protein1 (MRP1) expression and on activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). RESULTS: CuNG increased ROS generation and reduced MRP1 expression in EAC/Dox cells while only temporarily depleted glutathione (GSH) within 2 h in heart, kidney, liver and lung of EAC/Dox bearing mice, which were restored within 24 h. The level of liver Cu was observed to be inversely proportional to the level of GSH. Moreover, CuNG modulated SOD, CAT and GPx in different organs and thereby reduced oxidative stress. Thus nontoxic dose of CuNG may be utilized to reduce MRP1 expression and thus sensitize EAC/Dox cells to standard chemotherapy. Moreover, CuNG modulated SOD, CAT and and GPx activities to reduce oxidative stress in some vital organs of EAC/Dox bearing mice. CuNG treatment also helped to recover liver and renal function in EAC/Dox bearing mice. CONCLUSION: Based on our studies, we conclude that CuNG may be a promising candidate to sensitize drug resistant cancers in the clinic.
Date of acceptance
2006
Autoren
  • Ananda Mookerjee
  • Jayati Mookerjee Basu
  • Surajit Majumder
  • Shilpak Chatterjee
  • Gouri S Panda
  • Pranabananda Dutta
  • Smarajit Pal
  • Pratima Mukherjee
  • Thomas Efferth
  • Syamal Roy
  • Soumitra K Choudhuri
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/17107616
DOI
10.1186/1471-2407-6-267
eISSN
1471-2407
Externe Identifier
  • PubMed Central ID: PMC1660546
Zeitschrift
BMC Cancer
Schlüsselwörter
  • Animals
  • Antibiotics, Antineoplastic
  • Carcinoma, Ehrlich Tumor
  • Catalase
  • Doxorubicin
  • Drug Resistance, Neoplasm
  • Glutathione Peroxidase
  • Glycine
  • Mice
  • Multidrug Resistance-Associated Proteins
  • Organometallic Compounds
  • Reactive Oxygen Species
  • Superoxide Dismutase
Sprache
eng
Country
England
Paginierung
267
PII
1471-2407-6-267
Datum der Veröffentlichung
2006
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2006
Titel
A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
6

Data source: PubMed

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