Effect of cantharidin, cephalotaxine and homoharringtonine on "in vitro" models of hepatitis B virus (HBV) and bovine viral diarrhoea virus (BVDV) replication

Autoren

Marta Romero
Maria Serrano
Thomas Efferth
Marcelino Alvarez
Jose Marin

DOI

10.1055/s-2007-967184

eISSN

1439-0221

ISSN

0032-0943

Ausgabe der Veröffentlichung

6

Zeitschrift

Planta Medica

Sprache

en

Paginierung

552 - 558

Datum der Veröffentlichung

2007

Status

Published

Herausgeber

Georg Thieme Verlag KG

Herausgeber URL

http://dx.doi.org/10.1055/s-2007-967184

Datum der Datenerfassung

2017

Titel

Effect of Cantharidin, Cephalotaxine and Homoharringtonine on ”<i>in vitro</i>” Models of Hepatitis B Virus (HBV) and Bovine Viral Diarrhoea Virus (BVDV) Replication

Ausgabe der Zeitschrift

73

Data source: Crossref

Abstract

The effect as antiviral agents versus viral hepatitis B and C of three compounds purified from natural products commonly used as remedies in traditional Chinese medicine, cantharidin, cephalotaxine and homoharingtonine, was investigated. To assess the activity of these compounds against flavivirus, we used bovine viral diarrhoea virus (BVDV) as a surrogate for hepatitis C virus (HCV). Anti-BVDV activity was determined by reduction in BVDV-RNA production and protection of infected embryonic bovine trachea (EBTr) cells against the cytopathic effect of BVDV. The effect versus hepatitis B virus (HBV) was investigated by measuring HBsAg and HBV-DNA release from hepatoblastoma HepG2 2.2.15 cells infected with HBV. As positive control we used the standard anti-HBV and anti-HCV drugs, lamivudine and ribavirin, respectively. Up to 100 microM lamivudine and ribavirin did not induce cell toxicity, whereas they induced dose-dependent anti-HBV and anti-BVDV effects, respectively. In the same range, cantharidin, cephalotaxine and homoharringtonine induced toxicity in EBTr cells and had no protective effect against BVDV. In contrast, they were able to inhibit HBV production at concentrations 10- to 100-fold lower than those inducing cell toxicity, which suggests that they are useless for the treatment of infection by flaviviruses, but potentially useful in combined therapy against hepatitis B.

Addresses

Laboratory of Experimental Hepatology and Drug Targeting (HEVEFARM), University of Salamanca, Salamanca, Spain.

Autoren

Marta R Romero
Maria A Serrano
Thomas Efferth
Thomas Efferth
Marcelino Alvarez
Jose J Marin

DOI

10.1055/s-2007-967184

eISSN

1439-0221

Externe Identifier

PubMed Identifier: 17458779

Open access

false

ISSN

0032-0943

Ausgabe der Veröffentlichung

6

Zeitschrift

Planta medica

Schlüsselwörter

Animals
Cattle
Humans
Hepatitis B virus
Diarrhea Viruses, Bovine Viral
Cephalotaxus
Trees
Harringtonines
Cantharidin
Plant Extracts
Antiviral Agents
Phytotherapy
Microbial Sensitivity Tests
Virus Replication
In Vitro Techniques
Homoharringtonine

Sprache

eng

Medium

Print-Electronic

Online publication date

2007

Paginierung

552 - 558

Datum der Veröffentlichung

2007

Status

Published

Datum der Datenerfassung

2007

Titel

Effect of cantharidin, cephalotaxine and homoharringtonine on "in vitro" models of hepatitis B virus (HBV) and bovine viral diarrhoea virus (BVDV) replication.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

73

Data source: Europe PubMed Central

Abstract

The effect as antiviral agents versus viral hepatitis B and C of three compounds purified from natural products commonly used as remedies in traditional Chinese medicine, cantharidin, cephalotaxine and homoharingtonine, was investigated. To assess the activity of these compounds against flavivirus, we used bovine viral diarrhoea virus (BVDV) as a surrogate for hepatitis C virus (HCV). Anti-BVDV activity was determined by reduction in BVDV-RNA production and protection of infected embryonic bovine trachea (EBTr) cells against the cytopathic effect of BVDV. The effect versus hepatitis B virus (HBV) was investigated by measuring HBsAg and HBV-DNA release from hepatoblastoma HepG2 2.2.15 cells infected with HBV. As positive control we used the standard anti-HBV and anti-HCV drugs, lamivudine and ribavirin, respectively. Up to 100 microM lamivudine and ribavirin did not induce cell toxicity, whereas they induced dose-dependent anti-HBV and anti-BVDV effects, respectively. In the same range, cantharidin, cephalotaxine and homoharringtonine induced toxicity in EBTr cells and had no protective effect against BVDV. In contrast, they were able to inhibit HBV production at concentrations 10- to 100-fold lower than those inducing cell toxicity, which suggests that they are useless for the treatment of infection by flaviviruses, but potentially useful in combined therapy against hepatitis B.

Autoren

Marta R Romero
Maria A Serrano
Thomas Efferth
Marcelino Alvarez
Jose J Marin

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/17458779

DOI

10.1055/s-2007-967184

ISSN

0032-0943

Ausgabe der Veröffentlichung

6

Zeitschrift

Planta Med

Schlüsselwörter

Animals
Antiviral Agents
Cantharidin
Cattle
Cephalotaxus
Diarrhea Viruses, Bovine Viral
Harringtonines
Hepatitis B virus
Homoharringtonine
Humans
In Vitro Techniques
Microbial Sensitivity Tests
Phytotherapy
Plant Extracts
Trees
Virus Replication

Sprache

eng

Country

Germany

Paginierung

552 - 558

Datum der Veröffentlichung

2007

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2007

Titel

Effect of cantharidin, cephalotaxine and homoharringtonine on "in vitro" models of hepatitis B virus (HBV) and bovine viral diarrhoea virus (BVDV) replication.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

73

Data source: PubMed

Effect of cantharidin, cephalotaxine and homoharringtonine on "in vitro" models of hepatitis B virus (HBV) and bovine viral diarrhoea virus (BVDV) replication

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