Separation of 7-xylosyl-10-deacetyl paclitaxel and 10-deacetylbaccatin III from the remainder extracts free of paclitaxel using macroporous resins

Publication type:
Journal article
Metadata:
Autoren
  • Yujie Fu
  • Yuangang Zu
  • Shuangming Li
  • Rui Sun
  • Thomas Efferth
  • Wei Liu
  • Shougang Jiang
  • Hao Luo
  • Ying Wang
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000252370700008&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.chroma.2007.11.020
eISSN
1873-3778
Externe Identifier
  • Clarivate Analytics Document Solution ID: 251KA
  • PubMed Identifier: 18054030
ISSN
0021-9673
Ausgabe der Veröffentlichung
1
Zeitschrift
JOURNAL OF CHROMATOGRAPHY A
Schlüsselwörter
  • 7-xylosyl-10-deacetyl paclitaxel
  • 10-deacetylbaccatin III
  • separation
  • macroporous resins
Paginierung
77 - 86
Datum der Veröffentlichung
2008
Status
Published
Titel
Separation of 7-xylosyl-10-deacetyl paclitaxel and 10-deacetylbaccatin III from the remainder extracts free of paclitaxel using macroporous resins
Sub types
  • Article
Ausgabe der Zeitschrift
1177

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Yujie Fu
  • Yuangang Zu
  • Shuangming Li
  • Rui Sun
  • Thomas Efferth
  • Wei Liu
  • Shougang Jiang
  • Hao Luo
  • Ying Wang
DOI
10.1016/j.chroma.2007.11.020
ISSN
0021-9673
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of Chromatography A
Sprache
en
Paginierung
77 - 86
Datum der Veröffentlichung
2008
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.chroma.2007.11.020
Datum der Datenerfassung
2022
Titel
Separation of 7-xylosyl-10-deacetyl paclitaxel and 10-deacetylbaccatin III from the remainder extracts free of paclitaxel using macroporous resins
Ausgabe der Zeitschrift
1177

Data source: Crossref

Abstract
The separation and enrichment of 10-deacetylbaccatin III (10-DAB III) and 7-xylosyl-10-deacetyl paclitaxel were studied on seven macroporous resins with special structures. The performance of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III on macroporous resins including AB-8, ADS-17, ADS-21, ADS-31, ADS-8, H1020 and NKA-II was compared according to their adsorption and desorption properties. AB-8 provided a much higher adsorption capacity for 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III than other resins, and its adsorption data fitted well to the Langmuir and Freundlich isotherm. According to the adsorption and desorption capacities and the adsorption isotherms, AB-8 demonstrated a remarkable capability for the preparative separation of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III from the remainder extracts free of paclitaxel. In order to optimize parameters of separation, dynamic adsorption and desorption experiments were carried out on the columns packed with AB-8 resin. The optimal conditions were: the processing volume 15 BV; concentrations of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III in feed solution 0.0657 mg/mL and 0.1494 mg/mL; flow rate 1 mL/min; temperature 35 degrees C. The gradient elution program was as follows: 30% ethanol for 3 BV, then 80% of ethanol for 6 BV, flow rate 1 mL/min. After the AB-8 resin treatment, the contents of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III in the product had increased from 0.053% and 0.2% to 3.34% and 1.69%, which were 62.43-fold and 8.54-fold of those in the untreated extracts, respectively, and the recoveries of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III were 85.85% and 52.78%. The performance achieved good separation and higher recovery of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III from remainder extracts free of paclitaxel by using AB-8 resin. It is a fast and effective method for the separation and enrichment of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Autoren
  • Yujie Fu
  • Yuangang Zu
  • Shuangming Li
  • Rui Sun
  • Thomas Efferth
  • Thomas Efferth
  • Wei Liu
  • Shougang Jiang
  • Hao Luo
  • Ying Wang
DOI
10.1016/j.chroma.2007.11.020
eISSN
1873-3778
Externe Identifier
  • PubMed Identifier: 18054030
Open access
false
ISSN
0021-9673
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of chromatography. A
Schlüsselwörter
  • Taxoids
  • Plant Extracts
  • Chromatography, High Pressure Liquid
  • Spectrophotometry, Ultraviolet
  • Kinetics
  • Adsorption
  • Thermodynamics
  • Reference Standards
Sprache
eng
Medium
Print-Electronic
Online publication date
2007
Paginierung
77 - 86
Datum der Veröffentlichung
2008
Status
Published
Datum der Datenerfassung
2007
Titel
Separation of 7-xylosyl-10-deacetyl paclitaxel and 10-deacetylbaccatin III from the remainder extracts free of paclitaxel using macroporous resins.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
1177

Data source: Europe PubMed Central

Abstract
The separation and enrichment of 10-deacetylbaccatin III (10-DAB III) and 7-xylosyl-10-deacetyl paclitaxel were studied on seven macroporous resins with special structures. The performance of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III on macroporous resins including AB-8, ADS-17, ADS-21, ADS-31, ADS-8, H1020 and NKA-II was compared according to their adsorption and desorption properties. AB-8 provided a much higher adsorption capacity for 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III than other resins, and its adsorption data fitted well to the Langmuir and Freundlich isotherm. According to the adsorption and desorption capacities and the adsorption isotherms, AB-8 demonstrated a remarkable capability for the preparative separation of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III from the remainder extracts free of paclitaxel. In order to optimize parameters of separation, dynamic adsorption and desorption experiments were carried out on the columns packed with AB-8 resin. The optimal conditions were: the processing volume 15 BV; concentrations of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III in feed solution 0.0657 mg/mL and 0.1494 mg/mL; flow rate 1 mL/min; temperature 35 degrees C. The gradient elution program was as follows: 30% ethanol for 3 BV, then 80% of ethanol for 6 BV, flow rate 1 mL/min. After the AB-8 resin treatment, the contents of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III in the product had increased from 0.053% and 0.2% to 3.34% and 1.69%, which were 62.43-fold and 8.54-fold of those in the untreated extracts, respectively, and the recoveries of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III were 85.85% and 52.78%. The performance achieved good separation and higher recovery of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III from remainder extracts free of paclitaxel by using AB-8 resin. It is a fast and effective method for the separation and enrichment of 7-xylosyl-10-deacetyl paclitaxel and 10-DAB III.
Date of acceptance
2007
Autoren
  • Yujie Fu
  • Yuangang Zu
  • Shuangming Li
  • Rui Sun
  • Thomas Efferth
  • Wei Liu
  • Shougang Jiang
  • Hao Luo
  • Ying Wang
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/18054030
DOI
10.1016/j.chroma.2007.11.020
ISSN
0021-9673
Ausgabe der Veröffentlichung
1
Zeitschrift
J Chromatogr A
Schlüsselwörter
  • Adsorption
  • Chromatography, High Pressure Liquid
  • Kinetics
  • Plant Extracts
  • Reference Standards
  • Spectrophotometry, Ultraviolet
  • Taxoids
  • Thermodynamics
Sprache
eng
Country
Netherlands
Paginierung
77 - 86
PII
S0021-9673(07)01917-6
Datum der Veröffentlichung
2008
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2008
Titel
Separation of 7-xylosyl-10-deacetyl paclitaxel and 10-deacetylbaccatin III from the remainder extracts free of paclitaxel using macroporous resins.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
1177

Data source: PubMed

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