Investigation of antibacterial activity of rosemary essential oil against propionibacterium acnes with atomic force microscopy

Autoren

Yujie Fu
Yuangang Zu
Liyan Chen
Thomas Efferth
Huili Liang
Zhiguo Liu
Wei Liu

DOI

10.1055/s-2007-981614

eISSN

1439-0221

ISSN

0032-0943

Ausgabe der Veröffentlichung

12

Zeitschrift

Planta Medica

Sprache

en

Paginierung

1275 - 1280

Datum der Veröffentlichung

2007

Status

Published

Herausgeber

Georg Thieme Verlag KG

Herausgeber URL

http://dx.doi.org/10.1055/s-2007-981614

Datum der Datenerfassung

2017

Titel

Investigation of Antibacterial Activity of Rosemary Essential Oil against <i>Propionibacterium acnes</i> with Atomic Force Microscopy

Ausgabe der Zeitschrift

73

Data source: Crossref

Abstract

In the present study, the antibacterial activity of rosemary (Rosmarinus officinalis L. Labiatae) essential oil against Propionibacterium acnes (P. acnes) was observed with atomic force microscopy (AFM). The MIC (minimal inhibitory concentration) value of rosemary essential oil against P.acnes was 0.56 mg/mL. Significant changes in morphology and size of P. acnes were observed by atomic force microscopy (AFM) in response to essential oil treatment. The essential oil first attached to the surface of P. acnes at low concentration, the width and height of the bacterial body became larger, whereas the length did not change considerably. With increasing concentration of the essential oil, the bacterial bodies were severely damaged. The length, width and height were all reduced, when the concentration was increased up to 64xMIC, the length, width and height were reduced by 42.56%, 92.00% and 41.58%, respectively. Furthermore, treated bacteria lost their native shape, the cell wall desquamated, and the cytoplasm leaked out of the bacterial body, finally leading to bacterial death. With the increasing time at MIC, the bacteria length was reduced at 8 h, the width and height gradually became smaller, the shape of the cell became distorted, and finally led to cell wall damage and bacterial death at 8 h. In conclusion, the AFM investigation of morphology and size of P. ACNES treated with rosemary essential oil represents a powerful technique, which can generally be applied to reveal the biological changing mechanisms of bacteria induced by antibacterial agents at the nanometer level.

Addresses

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China. yujie_fu2002@yahoo.com

Autoren

Yujie Fu
Yuangang Zu
Liyan Chen
Thomas Efferth
Thomas Efferth
Huili Liang
Zhiguo Liu
Wei Liu

DOI

10.1055/s-2007-981614

eISSN

1439-0221

Externe Identifier

PubMed Identifier: 17893831

Open access

false

ISSN

0032-0943

Ausgabe der Veröffentlichung

12

Zeitschrift

Planta medica

Schlüsselwörter

Humans
Propionibacterium acnes
Rosmarinus
Acne Vulgaris
Oils, Volatile
Anti-Bacterial Agents
Microscopy, Atomic Force
Microbial Sensitivity Tests

Sprache

eng

Medium

Print-Electronic

Online publication date

2007

Paginierung

1275 - 1280

Datum der Veröffentlichung

2007

Status

Published

Datum der Datenerfassung

2007

Titel

Investigation of antibacterial activity of rosemary essential oil against Propionibacterium acnes with atomic force microscopy.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

73

Data source: Europe PubMed Central

Abstract

In the present study, the antibacterial activity of rosemary (Rosmarinus officinalis L. Labiatae) essential oil against Propionibacterium acnes (P. acnes) was observed with atomic force microscopy (AFM). The MIC (minimal inhibitory concentration) value of rosemary essential oil against P.acnes was 0.56 mg/mL. Significant changes in morphology and size of P. acnes were observed by atomic force microscopy (AFM) in response to essential oil treatment. The essential oil first attached to the surface of P. acnes at low concentration, the width and height of the bacterial body became larger, whereas the length did not change considerably. With increasing concentration of the essential oil, the bacterial bodies were severely damaged. The length, width and height were all reduced, when the concentration was increased up to 64xMIC, the length, width and height were reduced by 42.56%, 92.00% and 41.58%, respectively. Furthermore, treated bacteria lost their native shape, the cell wall desquamated, and the cytoplasm leaked out of the bacterial body, finally leading to bacterial death. With the increasing time at MIC, the bacteria length was reduced at 8 h, the width and height gradually became smaller, the shape of the cell became distorted, and finally led to cell wall damage and bacterial death at 8 h. In conclusion, the AFM investigation of morphology and size of P. ACNES treated with rosemary essential oil represents a powerful technique, which can generally be applied to reveal the biological changing mechanisms of bacteria induced by antibacterial agents at the nanometer level.

Autoren

Yujie Fu
Yuangang Zu
Liyan Chen
Thomas Efferth
Huili Liang
Zhiguo Liu
Wei Liu

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/17893831

DOI

10.1055/s-2007-981614

ISSN

0032-0943

Ausgabe der Veröffentlichung

12

Zeitschrift

Planta Med

Schlüsselwörter

Acne Vulgaris
Anti-Bacterial Agents
Humans
Microbial Sensitivity Tests
Microscopy, Atomic Force
Oils, Volatile
Propionibacterium acnes
Rosmarinus

Sprache

eng

Country

Germany

Paginierung

1275 - 1280

Datum der Veröffentlichung

2007

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2008

Titel

Investigation of antibacterial activity of rosemary essential oil against Propionibacterium acnes with atomic force microscopy.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

73

Data source: PubMed

Investigation of antibacterial activity of rosemary essential oil against propionibacterium acnes with atomic force microscopy

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