Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC-MS

Abstract

AbstractA method based on ultrasonic extraction (USE) followed by LC‐MS is presented for the determination of vitexin and isovitexin in pigeonpea extracts in this study. The influential parameters of the USE procedure were optimized, and the optimal conditions were as follows: extraction solvent, 60% ethanol solution; liquid/solid ratio 10:1 (mL/g), extraction power, 250 W; temperature, 40–50°C; and three extraction cycles, each cycle 15 min. Validation of the USE method was performed in terms of repeatability and reproducibility. RSDs for extraction yields were lower than 5.85 and 8.09%, respectively. The LOD and LOQ of chromatographic determination were 0.96 and 3.2 ng/mL for vitexin and 0.84 and 2.8 ng/mL for isovitexin. The method was also successfully applied for the determination of vitexin and isovitexin in stems, leaves, and root extracts of pigeonpea. From all these results, we may conclude that the developed method is appropriate for the quality control of pigeonpea and other plant extract products developed from pigeonpea.

Autoren

Yujie Fu
Yuangang Zu
Wei Liu
Lin Zhang
Meihong Tong
Thomas Efferth
Yu Kong
Chunlian Hou
Liyan Chen

DOI

10.1002/jssc.200700312

eISSN

1615-9314

ISSN

1615-9306

Ausgabe der Veröffentlichung

2

Zeitschrift

Journal of Separation Science

Sprache

en

Online publication date

2008

Paginierung

268 - 275

Datum der Veröffentlichung

2008

Status

Published

Herausgeber

Wiley

Herausgeber URL

http://dx.doi.org/10.1002/jssc.200700312

Datum der Datenerfassung

2023

Titel

Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC‐MS

Ausgabe der Zeitschrift

31

Data source: Crossref

Abstract

A method based on ultrasonic extraction (USE) followed by LC-MS is presented for the determination of vitexin and isovitexin in pigeonpea extracts in this study. The influential parameters of the USE procedure were optimized, and the optimal conditions were as follows: extraction solvent, 60% ethanol solution; liquid/solid ratio 10:1 (mL/g), extraction power, 250 W; temperature, 40-50 degrees C; and three extraction cycles, each cycle 15 min. Validation of the USE method was performed in terms of repeatability and reproducibility. RSDs for extraction yields were lower than 5.85 and 8.09%, respectively. The LOD and LOQ of chromatographic determination were 0.96 and 3.2 ng/mL for vitexin and 0.84 and 2.8 ng/mL for isovitexin. The method was also successfully applied for the determination of vitexin and isovitexin in stems, leaves, and root extracts of pigeonpea. From all these results, we may conclude that the developed method is appropriate for the quality control of pigeonpea and other plant extract products developed from pigeonpea.

Addresses

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.

Autoren

Yujie Fu
Yuangang Zu
Wei Liu
Lin Zhang
Meihong Tong
Thomas Efferth
Thomas Efferth
Yu Kong
Chunlian Hou
Liyan Chen

DOI

10.1002/jssc.200700312

eISSN

1615-9314

Externe Identifier

PubMed Identifier: 18196523

Open access

false

ISSN

1615-9306

Ausgabe der Veröffentlichung

2

Zeitschrift

Journal of separation science

Schlüsselwörter

Cajanus
Apigenin
Chromatography, High Pressure Liquid
Temperature
Ultrasonics
Reference Standards
Mass Spectrometry

Sprache

eng

Medium

Print

Paginierung

268 - 275

Datum der Veröffentlichung

2008

Status

Published

Datum der Datenerfassung

2008

Titel

Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC-MS.

Sub types

Research Support, Non-U.S. Gov't
Validation Study
Journal Article

Ausgabe der Zeitschrift

31

Data source: Europe PubMed Central

Abstract

A method based on ultrasonic extraction (USE) followed by LC-MS is presented for the determination of vitexin and isovitexin in pigeonpea extracts in this study. The influential parameters of the USE procedure were optimized, and the optimal conditions were as follows: extraction solvent, 60% ethanol solution; liquid/solid ratio 10:1 (mL/g), extraction power, 250 W; temperature, 40-50 degrees C; and three extraction cycles, each cycle 15 min. Validation of the USE method was performed in terms of repeatability and reproducibility. RSDs for extraction yields were lower than 5.85 and 8.09%, respectively. The LOD and LOQ of chromatographic determination were 0.96 and 3.2 ng/mL for vitexin and 0.84 and 2.8 ng/mL for isovitexin. The method was also successfully applied for the determination of vitexin and isovitexin in stems, leaves, and root extracts of pigeonpea. From all these results, we may conclude that the developed method is appropriate for the quality control of pigeonpea and other plant extract products developed from pigeonpea.

Autoren

Yujie Fu
Yuangang Zu
Wei Liu
Lin Zhang
Meihong Tong
Thomas Efferth
Yu Kong
Chunlian Hou
Liyan Chen

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/18196523

DOI

10.1002/jssc.200700312

ISSN

1615-9306

Ausgabe der Veröffentlichung

2

Zeitschrift

J Sep Sci

Schlüsselwörter

Apigenin
Cajanus
Chromatography, High Pressure Liquid
Mass Spectrometry
Reference Standards
Temperature
Ultrasonics

Sprache

eng

Country

Germany

Paginierung

268 - 275

Datum der Veröffentlichung

2008

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2008

Titel

Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC-MS.

Sub types

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Ausgabe der Zeitschrift

31

Data source: PubMed

Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC-MS

Tools