Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column

Publication type:
Journal article
Metadata:
Autoren
  • Yu-Jie Fu
  • Rui Sun
  • Yuan-Gang Zu
  • Shuang-Ming Li
  • Wei Liu
  • Thomas Efferth
  • Cheng-Bo Gu
  • Lin Zhang
  • Hao Luo
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000262191600007&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/bmc.1085
eISSN
1099-0801
Externe Identifier
  • Clarivate Analytics Document Solution ID: 390VK
  • PubMed Identifier: 18816506
ISSN
0269-3879
Ausgabe der Veröffentlichung
1
Zeitschrift
BIOMEDICAL CHROMATOGRAPHY
Schlüsselwörter
  • taxoids
  • HPLC
  • PFP column
  • Taxus
Paginierung
63 - 70
Datum der Veröffentlichung
2009
Status
Published
Titel
Simultaneous determination of main taxoids in <i>Taxus</i> needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column
Sub types
  • Article
Ausgabe der Zeitschrift
23

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:p>A simple and accurate RP‐HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10‐deacetylbaccatin III (10‐DAB III), 7‐xylosyl‐10‐deacetyltaxol (7‐xyl‐10‐DAT), 10‐deacetyltaxol (10‐DAT), cephalomannine and 7‐epi‐10‐deacetyltaxol (7‐epi‐10‐DAT), in the extracts from the needles of three <jats:italic>Taxus</jats:italic> species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD &lt; 2.61%), repeatability (RSD &lt; 2.92%) and recovery (95.19–104.47%). The above taxoids in the extracts of <jats:italic>Taxus cuspidata</jats:italic>, <jats:italic>T</jats:italic>.<jats:italic> chinensis</jats:italic> and <jats:italic>T</jats:italic>.<jats:italic> media</jats:italic> were analyzed with the developed RP‐HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10‐DAB III, 7‐xyl‐10‐DAT and 7‐epi‐10‐DAT appeared in <jats:italic>T</jats:italic>.<jats:italic> chinensis</jats:italic>, while <jats:italic>T</jats:italic>.<jats:italic> media</jats:italic> possessed the highest content of 10‐DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of <jats:italic>Taxus</jats:italic> species. Copyright © 2008 John Wiley &amp; Sons, Ltd.</jats:p>
Autoren
  • Yu‐Jie Fu
  • Rui Sun
  • Yuan‐Gang Zu
  • Shuang‐Ming Li
  • Wei Liu
  • Thomas Efferth
  • Cheng‐Bo Gu
  • Lin Zhang
  • Hao Luo
DOI
10.1002/bmc.1085
eISSN
1099-0801
ISSN
0269-3879
Ausgabe der Veröffentlichung
1
Zeitschrift
Biomedical Chromatography
Sprache
en
Online publication date
2008
Paginierung
63 - 70
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/bmc.1085
Datum der Datenerfassung
2023
Titel
Simultaneous determination of main taxoids in <i>Taxus</i> needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column
Ausgabe der Zeitschrift
23

Data source: Crossref

Abstract
A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.
Autoren
  • Yu-Jie Fu
  • Rui Sun
  • Yuan-Gang Zu
  • Shuang-Ming Li
  • Wei Liu
  • Thomas Efferth
  • Thomas Efferth
  • Cheng-Bo Gu
  • Lin Zhang
  • Hao Luo
DOI
10.1002/bmc.1085
eISSN
1099-0801
Externe Identifier
  • PubMed Identifier: 18816506
Open access
false
ISSN
0269-3879
Ausgabe der Veröffentlichung
1
Zeitschrift
Biomedical chromatography : BMC
Schlüsselwörter
  • Taxus
  • Taxoids
  • Chromatography, High Pressure Liquid
  • Molecular Structure
  • Solid Phase Extraction
Sprache
eng
Medium
Print
Paginierung
63 - 70
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2008
Titel
Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: Europe PubMed Central

Abstract
A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.
Autoren
  • Yu-Jie Fu
  • Rui Sun
  • Yuan-Gang Zu
  • Shuang-Ming Li
  • Wei Liu
  • Thomas Efferth
  • Cheng-Bo Gu
  • Lin Zhang
  • Hao Luo
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/18816506
DOI
10.1002/bmc.1085
eISSN
1099-0801
Ausgabe der Veröffentlichung
1
Zeitschrift
Biomed Chromatogr
Schlüsselwörter
  • Chromatography, High Pressure Liquid
  • Molecular Structure
  • Solid Phase Extraction
  • Taxoids
  • Taxus
Sprache
eng
Country
England
Paginierung
63 - 70
Datum der Veröffentlichung
2009
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
23

Data source: PubMed

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