Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Shuangming Li
- Yujie Fu
- Yuangang Zu
- Rui Sun
- Ying Wang
- Lin Zhang
- Hao Luo
- Chengbo Gu
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000263088000011&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jpba.2008.10.006
- eISSN
- 1873-264X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 403MZ
- PubMed Identifier: 19036549
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
- Schlüsselwörter
- Paclitaxel
- Taxoids
- Determination
- High-performance liquid chromatography
- Tandem mass spectrometry
- Paginierung
- 81 - 89
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- Determination of paclitaxel and other six taxoids in <i>Taxus</i> species by high-performance liquid chromatography-tandem mass spectrometry
- Sub types
- Article
- Ausgabe der Zeitschrift
- 49
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Shuangming Li
- Yujie Fu
- Yuangang Zu
- Rui Sun
- Ying Wang
- Lin Zhang
- Hao Luo
- Chengbo Gu
- Thomas Efferth
- DOI
- 10.1016/j.jpba.2008.10.006
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Journal of Pharmaceutical and Biomedical Analysis
- Sprache
- en
- Paginierung
- 81 - 89
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jpba.2008.10.006
- Datum der Datenerfassung
- 2022
- Titel
- Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography–tandem mass spectrometry
- Ausgabe der Zeitschrift
- 49
Data source: Crossref
- Abstract
- A method of high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) has been developed for the trace analysis of paclitaxel and other six taxoids in three Taxus species including Taxus cuspidata, Taxus media and Taxus chinensis var. mairiei. Seven taxoids were separated using a gradient mode on an Eclipse XDB-C18 column (4.6x150 mm; i.d., 5 microm) at 20 degrees C. The compound separations were detected by an API 3000 mass spectrometer equipped with a TurboIonSpray interface. The compounds were detected using electrospray ionization (ESI) in positive-ion mode and quantified by multiple-reaction monitoring (MRM) mode using the transition mass of m/z 567.4-->445.4, 609.3-->549.5, 944.9-->286.4, 812.6-->286.1, 832.8-->264.1, 854.4-->286.1, and 812.6-->286.1 for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. The ranges of limit of quantitation (LOQ) for taxoids were 32, 14, 26, 14, 20, 32, and 26 ng/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. Linearity was confirmed over the whole calibration range (0.07-45, 0.058-37.5, 0.058-37.5, 0.056-36.3, 0.053-33.8, 0.057-37.5, and 0.06-38.8 microg/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively) with coefficients higher than 0.9903. The inter- and intra-day precision of taxoids ranged from 2.86% to 6.31% for retention time and ranged from 3.91% to 7.33% for peak area. The recovery rates of this method were higher than 94.32% for 10-DAB III, 94.68% for baccatin III, 93.65% for 7-xyl-10-DAT, 93.29% for 10-DAT, 92.91% for cephalomannine, 93.41% for paclitaxel, and 93.06% for 7-epi-10-DAT, respectively.
- Addresses
- Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
- Autoren
- Shuangming Li
- Yujie Fu
- Yuangang Zu
- Rui Sun
- Ying Wang
- Lin Zhang
- Hao Luo
- Chengbo Gu
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.jpba.2008.10.006
- eISSN
- 1873-264X
- Externe Identifier
- PubMed Identifier: 19036549
- Open access
- false
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Journal of pharmaceutical and biomedical analysis
- Schlüsselwörter
- Taxus
- Taxoids
- Paclitaxel
- Antineoplastic Agents, Phytogenic
- Calibration
- Chromatography, High Pressure Liquid
- Spectrometry, Mass, Electrospray Ionization
- Sensitivity and Specificity
- Feasibility Studies
- Reproducibility of Results
- Species Specificity
- Molecular Structure
- Tandem Mass Spectrometry
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2008
- Paginierung
- 81 - 89
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2008
- Titel
- Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 49
Data source: Europe PubMed Central
- Abstract
- A method of high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) has been developed for the trace analysis of paclitaxel and other six taxoids in three Taxus species including Taxus cuspidata, Taxus media and Taxus chinensis var. mairiei. Seven taxoids were separated using a gradient mode on an Eclipse XDB-C18 column (4.6x150 mm; i.d., 5 microm) at 20 degrees C. The compound separations were detected by an API 3000 mass spectrometer equipped with a TurboIonSpray interface. The compounds were detected using electrospray ionization (ESI) in positive-ion mode and quantified by multiple-reaction monitoring (MRM) mode using the transition mass of m/z 567.4-->445.4, 609.3-->549.5, 944.9-->286.4, 812.6-->286.1, 832.8-->264.1, 854.4-->286.1, and 812.6-->286.1 for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. The ranges of limit of quantitation (LOQ) for taxoids were 32, 14, 26, 14, 20, 32, and 26 ng/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. Linearity was confirmed over the whole calibration range (0.07-45, 0.058-37.5, 0.058-37.5, 0.056-36.3, 0.053-33.8, 0.057-37.5, and 0.06-38.8 microg/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively) with coefficients higher than 0.9903. The inter- and intra-day precision of taxoids ranged from 2.86% to 6.31% for retention time and ranged from 3.91% to 7.33% for peak area. The recovery rates of this method were higher than 94.32% for 10-DAB III, 94.68% for baccatin III, 93.65% for 7-xyl-10-DAT, 93.29% for 10-DAT, 92.91% for cephalomannine, 93.41% for paclitaxel, and 93.06% for 7-epi-10-DAT, respectively.
- Date of acceptance
- 2008
- Autoren
- Shuangming Li
- Yujie Fu
- Yuangang Zu
- Rui Sun
- Ying Wang
- Lin Zhang
- Hao Luo
- Chengbo Gu
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/19036549
- DOI
- 10.1016/j.jpba.2008.10.006
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- J Pharm Biomed Anal
- Schlüsselwörter
- Antineoplastic Agents, Phytogenic
- Calibration
- Chromatography, High Pressure Liquid
- Feasibility Studies
- Molecular Structure
- Paclitaxel
- Reproducibility of Results
- Sensitivity and Specificity
- Species Specificity
- Spectrometry, Mass, Electrospray Ionization
- Tandem Mass Spectrometry
- Taxoids
- Taxus
- Sprache
- eng
- Country
- England
- Paginierung
- 81 - 89
- PII
- S0731-7085(08)00567-0
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2009
- Titel
- Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 49
Data source: PubMed
- Beziehungen:
- Property of