Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC-MS

Publication type:
Journal article
Metadata:
Autoren
  • Yuangang Zu
  • Mingming Yan
  • Yujie Fu
  • Wei Liu
  • Lin Zhang
  • Chengbo Gu
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000264082100006&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/jssc.200800499
eISSN
1615-9314
Externe Identifier
  • Clarivate Analytics Document Solution ID: 417NF
  • PubMed Identifier: 19160368
ISSN
1615-9306
Ausgabe der Veröffentlichung
4
Zeitschrift
JOURNAL OF SEPARATION SCIENCE
Schlüsselwörter
  • Astragalosides
  • Jinqi Jiangtang tablets
  • LC-MS/MS
  • MRM
  • Radix Astragali
Paginierung
517 - 525
Datum der Veröffentlichung
2009
Status
Published
Titel
Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC-MS
Sub types
  • Article
Ausgabe der Zeitschrift
32

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:p>In the present study, a liquid chromatography–tandem mass spectrometry method was developed for the separation and simultaneous quantification of astragalosides I–IV in samples of Radix Astragali and a medicinal product thereof (Jinqi Jiangtang tablets). Chromatographic separation was achieved on an Agilent Eclipse XDB (ODS)‐C18 column with a mobile phase consisting of acetonitrile and 0.05% formic acid aqueous solution by use of an efficient 17‐min program. A triple quadrupole mass spectrometer was operated in positive ionization mode with multiple reaction monitoring for the detection of four astragalosides. The saponin ginsenoside Rg1 (similar structure to astralagosides) was used as an internal standard. All calibration curves showed excellent linear regressions (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> ⪈ 0.9912) within the range of tested concentrations. The intra‐ and inter‐day variations were below 4.57% in terms of RSD. The recoveries were 94.38–103.53% with RSD of 1.39–3.58% for spiked Radix Astragali samples. The method was successfully used for the analysis of samples of Radix Astragali and Jinqi Jiangtang tablets. In conclusion, we have developed a rapid, efficient, and accurate LC–MS/MS method for the detection of astragalosides, which can be applied for quality control of Radix Astragali and related medicinal products.</jats:p>
Autoren
  • Yuangang Zu
  • Mingming Yan
  • Yujie Fu
  • Wei Liu
  • Lin Zhang
  • Chengbo Gu
  • Thomas Efferth
DOI
10.1002/jssc.200800499
eISSN
1615-9314
ISSN
1615-9306
Ausgabe der Veröffentlichung
4
Zeitschrift
Journal of Separation Science
Sprache
en
Online publication date
2009
Paginierung
517 - 525
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/jssc.200800499
Datum der Datenerfassung
2023
Titel
Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC–MS
Ausgabe der Zeitschrift
32

Data source: Crossref

Abstract
In the present study, a liquid chromatography-tandem mass spectrometry method was developed for the separation and simultaneous quantification of astragalosides I-IV in samples of Radix Astragali and a medicinal product thereof (Jinqi Jiangtang tablets). Chromatographic separation was achieved on an Agilent Eclipse XDB (ODS)-C18 column with a mobile phase consisting of acetonitrile and 0.05% formic acid aqueous solution by use of an efficient 17-min program. A triple quadrupole mass spectrometer was operated in positive ionization mode with multiple reaction monitoring for the detection of four astragalosides. The saponin ginsenoside Rg1 (similar structure to astralagosides) was used as an internal standard. All calibration curves showed excellent linear regressions (r(2) greater, not dbl equals 0.9912) within the range of tested concentrations. The intra- and inter-day variations were below 4.57% in terms of RSD. The recoveries were 94.38-103.53% with RSD of 1.39-3.58% for spiked Radix Astragali samples. The method was successfully used for the analysis of samples of Radix Astragali and Jinqi Jiangtang tablets. In conclusion, we have developed a rapid, efficient, and accurate LC-MS/MS method for the detection of astragalosides, which can be applied for quality control of Radix Astragali and related medicinal products.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Autoren
  • Yuangang Zu
  • Mingming Yan
  • Yujie Fu
  • Wei Liu
  • Lin Zhang
  • Chengbo Gu
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1002/jssc.200800499
eISSN
1615-9314
Externe Identifier
  • PubMed Identifier: 19160368
Open access
false
ISSN
1615-9306
Ausgabe der Veröffentlichung
4
Zeitschrift
Journal of separation science
Schlüsselwörter
  • Astragalus Plant
  • Triterpenes
  • Saponins
  • Drugs, Chinese Herbal
  • Chromatography, Liquid
  • Molecular Structure
  • Tandem Mass Spectrometry
Sprache
eng
Medium
Print
Paginierung
517 - 525
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2009
Titel
Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC-MS.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
32

Data source: Europe PubMed Central

Abstract
In the present study, a liquid chromatography-tandem mass spectrometry method was developed for the separation and simultaneous quantification of astragalosides I-IV in samples of Radix Astragali and a medicinal product thereof (Jinqi Jiangtang tablets). Chromatographic separation was achieved on an Agilent Eclipse XDB (ODS)-C18 column with a mobile phase consisting of acetonitrile and 0.05% formic acid aqueous solution by use of an efficient 17-min program. A triple quadrupole mass spectrometer was operated in positive ionization mode with multiple reaction monitoring for the detection of four astragalosides. The saponin ginsenoside Rg1 (similar structure to astralagosides) was used as an internal standard. All calibration curves showed excellent linear regressions (r(2) greater, not dbl equals 0.9912) within the range of tested concentrations. The intra- and inter-day variations were below 4.57% in terms of RSD. The recoveries were 94.38-103.53% with RSD of 1.39-3.58% for spiked Radix Astragali samples. The method was successfully used for the analysis of samples of Radix Astragali and Jinqi Jiangtang tablets. In conclusion, we have developed a rapid, efficient, and accurate LC-MS/MS method for the detection of astragalosides, which can be applied for quality control of Radix Astragali and related medicinal products.
Autoren
  • Yuangang Zu
  • Mingming Yan
  • Yujie Fu
  • Wei Liu
  • Lin Zhang
  • Chengbo Gu
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19160368
DOI
10.1002/jssc.200800499
eISSN
1615-9314
Ausgabe der Veröffentlichung
4
Zeitschrift
J Sep Sci
Schlüsselwörter
  • Astragalus Plant
  • Chromatography, Liquid
  • Drugs, Chinese Herbal
  • Molecular Structure
  • Saponins
  • Tandem Mass Spectrometry
  • Triterpenes
Sprache
eng
Country
Germany
Paginierung
517 - 525
Datum der Veröffentlichung
2009
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC-MS.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
32

Data source: PubMed

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