Cytotoxic Activity of Isoliquiritigenin towards CCRF-CEM Leukemia Cells and its Effect on DNA Damage
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Yuangang Zu
- Xia Liu
- Yujie Fu
- Xiaoguang Shi
- Nan Wu
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000269693200008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1055/s-0029-1185479
- eISSN
- 1439-0221
- Externe Identifier
- Clarivate Analytics Document Solution ID: 492WL
- PubMed Identifier: 19291608
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- PLANTA MEDICA
- Schlüsselwörter
- isoquiritigenin (ISL)
- anticancer activity
- CCRF-CEM cell line
- DNA damage
- Paginierung
- 1134 - 1140
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- Cytotoxic Activity of Isoliquiritigenin towards CCRF-CEM Leukemia Cells and its Effect on DNA Damage
- Sub types
- Article
- Ausgabe der Zeitschrift
- 75
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Yuangang Zu
- Xia Liu
- Yujie Fu
- Xiaoguang Shi
- Nan Wu
- Liping Yao
- Thomas Efferth
- DOI
- 10.1055/s-0029-1185479
- eISSN
- 1439-0221
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Planta Medica
- Sprache
- en
- Online publication date
- 2009
- Paginierung
- 1134 - 1140
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Georg Thieme Verlag KG
- Herausgeber URL
- http://dx.doi.org/10.1055/s-0029-1185479
- Datum der Datenerfassung
- 2017
- Titel
- Cytotoxic Activity of Isoliquiritigenin towards CCRF‐CEM Leukemia Cells and its Effect on DNA Damage
- Ausgabe der Zeitschrift
- 75
Data source: Crossref
- Abstract
- The cytotoxic activity of isoliquiritigenin (ISL) towards CCRF-CEM human T-cell leukemia cells and DNA damage induction were investigated in the present study. The anti-proliferative effect of ISL on CCRF-CEM cell line IN VITRO was analyzed. In order to further explore the underlying mechanism of cell growth inhibition of ISL towards CCRF-CEM cell line, the cell cycle distribution and mitochondrial membrane potential (DeltaPsim) disruption were measured. ISL exerted significant activity towards CCRF-CEM cells. The growth inhibitory ratio was concentration- and time-dependent with an IC(50) value of 18.38 microM. The cell cycle was arrested in the G2/M phase upon treatment with low doses of ISL. Mitochondrial membrane potential (DeltaPsim) disruption was observed at low ISL doses. The effect of ISL on DNA damage was analyzed by agarose gel electrophoresis and atomic force microscopy (AFM). Either ISL or Cu2+ failed to cause pBR322 DNA damage. However, plasmid DNA was damaged after treatment with ISL in the presence of Cu2+. Two forms of plasmid DNA closed circular and linear forms were visualized by AFM.
- Addresses
- Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, P. R. China.
- Autoren
- Yuangang Zu
- Xia Liu
- Yujie Fu
- Xiaoguang Shi
- Nan Wu
- Liping Yao
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1055/s-0029-1185479
- eISSN
- 1439-0221
- Externe Identifier
- PubMed Identifier: 19291608
- Open access
- false
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Planta medica
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- DNA Damage
- Chalcones
- Antineoplastic Agents
- Microscopy, Atomic Force
- Cell Division
- G2 Phase
- Cell Proliferation
- Membrane Potentials
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2009
- Paginierung
- 1134 - 1140
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2009
- Titel
- Cytotoxic activity of isoliquiritigenin towards CCRF-CEM leukemia cells and its effect on DNA damage.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 75
Data source: Europe PubMed Central
- Abstract
- The cytotoxic activity of isoliquiritigenin (ISL) towards CCRF-CEM human T-cell leukemia cells and DNA damage induction were investigated in the present study. The anti-proliferative effect of ISL on CCRF-CEM cell line IN VITRO was analyzed. In order to further explore the underlying mechanism of cell growth inhibition of ISL towards CCRF-CEM cell line, the cell cycle distribution and mitochondrial membrane potential (DeltaPsim) disruption were measured. ISL exerted significant activity towards CCRF-CEM cells. The growth inhibitory ratio was concentration- and time-dependent with an IC(50) value of 18.38 microM. The cell cycle was arrested in the G2/M phase upon treatment with low doses of ISL. Mitochondrial membrane potential (DeltaPsim) disruption was observed at low ISL doses. The effect of ISL on DNA damage was analyzed by agarose gel electrophoresis and atomic force microscopy (AFM). Either ISL or Cu2+ failed to cause pBR322 DNA damage. However, plasmid DNA was damaged after treatment with ISL in the presence of Cu2+. Two forms of plasmid DNA closed circular and linear forms were visualized by AFM.
- Autoren
- Yuangang Zu
- Xia Liu
- Yujie Fu
- Xiaoguang Shi
- Nan Wu
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/19291608
- DOI
- 10.1055/s-0029-1185479
- eISSN
- 1439-0221
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Planta Med
- Schlüsselwörter
- Antineoplastic Agents
- Cell Division
- Cell Line, Tumor
- Cell Proliferation
- Chalcones
- DNA Damage
- G2 Phase
- Humans
- Membrane Potentials
- Microscopy, Atomic Force
- Sprache
- eng
- Country
- Germany
- Paginierung
- 1134 - 1140
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2009
- Titel
- Cytotoxic activity of isoliquiritigenin towards CCRF-CEM leukemia cells and its effect on DNA damage.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 75
Data source: PubMed
- Beziehungen:
- Property of