Lipid rafts play an important role in the vesicular stomatitis virus life cycle

Publication type:
Journal article
Metadata:
Autoren
  • W Wang
  • YJ Fu
  • YG Zu
  • N Wu
  • J Reichling
  • T Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000264871500007&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/s00705-009-0348-2
eISSN
1432-8798
Externe Identifier
  • Clarivate Analytics Document Solution ID: 428SY
  • PubMed Identifier: 19288237
ISSN
0304-8608
Ausgabe der Veröffentlichung
4
Zeitschrift
ARCHIVES OF VIROLOGY
Paginierung
595 - 600
Datum der Veröffentlichung
2009
Status
Published
Titel
Lipid rafts play an important role in the vesicular stomatitis virus life cycle
Sub types
  • Article
Ausgabe der Zeitschrift
154

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • W Wang
  • YJ Fu
  • YG Zu
  • N Wu
  • J Reichling
  • T Efferth
DOI
10.1007/s00705-009-0348-2
eISSN
1432-8798
ISSN
0304-8608
Ausgabe der Veröffentlichung
4
Zeitschrift
Archives of Virology
Sprache
en
Online publication date
2009
Paginierung
595 - 600
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1007/s00705-009-0348-2
Datum der Datenerfassung
2021
Titel
Lipid rafts play an important role in the vesicular stomatitis virus life cycle
Ausgabe der Zeitschrift
154

Data source: Crossref

Abstract
Lipid rafts are involved in the life cycle of many viruses. In this study, we investigated the role of lipids in the life cycle of vesicular stomatitis virus (VSV). Cholesterol depletion by pretreatment of BHK cells or VSV particles with methyl-beta-cyclodextrin (MbetaCD), a cholesterol-sequestering drug, inhibited the production of VSV dramatically. This effect was reversible, and virus production was restored by the addition of cholesterol, indicating that the reduction was caused by the loss of cholesterol in the cell membrane and virus, respectively. Cholesterol depletion at the adsorption stage also reduced the production of VSV significantly, but in contrast, only had a limited effect on virus production at the post-entry stage. Inhibition of sphingomyelin by myriocin treatment only showed a minor effect on VSV production. However, reduction of cholesterol and sphingomyelin at the same time dramatically reduced VSV production, showed a significant synergistic effect. These results suggest that lipid rafts play an important role in the life cycle of VSV.
Addresses
  • Northeast Forestry University, Harbin, People's Republic of China.
Autoren
  • W Wang
  • YJ Fu
  • YG Zu
  • N Wu
  • J Reichling
  • T Efferth
  • T Efferth
DOI
10.1007/s00705-009-0348-2
eISSN
1432-8798
Externe Identifier
  • PubMed Identifier: 19288237
Open access
false
ISSN
0304-8608
Ausgabe der Veröffentlichung
4
Zeitschrift
Archives of virology
Schlüsselwörter
  • Cell Line
  • Membrane Microdomains
  • Animals
  • Vesiculovirus
  • beta-Cyclodextrins
  • Cholesterol
  • Sphingomyelins
  • Fatty Acids, Monounsaturated
  • Cell Survival
  • Cricetinae
  • Virus Internalization
Sprache
eng
Medium
Print-Electronic
Online publication date
2009
Paginierung
595 - 600
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2009
Titel
Lipid rafts play an important role in the vesicular stomatitis virus life cycle.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
154

Data source: Europe PubMed Central

Abstract
Lipid rafts are involved in the life cycle of many viruses. In this study, we investigated the role of lipids in the life cycle of vesicular stomatitis virus (VSV). Cholesterol depletion by pretreatment of BHK cells or VSV particles with methyl-beta-cyclodextrin (MbetaCD), a cholesterol-sequestering drug, inhibited the production of VSV dramatically. This effect was reversible, and virus production was restored by the addition of cholesterol, indicating that the reduction was caused by the loss of cholesterol in the cell membrane and virus, respectively. Cholesterol depletion at the adsorption stage also reduced the production of VSV significantly, but in contrast, only had a limited effect on virus production at the post-entry stage. Inhibition of sphingomyelin by myriocin treatment only showed a minor effect on VSV production. However, reduction of cholesterol and sphingomyelin at the same time dramatically reduced VSV production, showed a significant synergistic effect. These results suggest that lipid rafts play an important role in the life cycle of VSV.
Date of acceptance
2009
Autoren
  • W Wang
  • YJ Fu
  • YG Zu
  • N Wu
  • J Reichling
  • T Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19288237
DOI
10.1007/s00705-009-0348-2
eISSN
1432-8798
Ausgabe der Veröffentlichung
4
Zeitschrift
Arch Virol
Schlüsselwörter
  • Animals
  • Cell Line
  • Cell Survival
  • Cholesterol
  • Cricetinae
  • Fatty Acids, Monounsaturated
  • Membrane Microdomains
  • Sphingomyelins
  • Vesiculovirus
  • Virus Internalization
  • beta-Cyclodextrins
Sprache
eng
Country
Austria
Paginierung
595 - 600
Datum der Veröffentlichung
2009
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
Lipid rafts play an important role in the vesicular stomatitis virus life cycle.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
154

Data source: PubMed

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