The Role of Downstream Signaling Pathways of the Epidermal Growth Factor Receptor for Artesunate's Activity in Cancer Cells

Autoren

V Badireenath Konkimalla
James McCubrey
Thomas Efferth

Autoren-URL

https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000209702604307&DestLinkType=FullRecord&DestApp=WOS_CPL

eISSN

1538-7445

Externe Identifier

Clarivate Analytics Document Solution ID: V43TB

ISSN

0008-5472

Zeitschrift

CANCER RESEARCH

Datum der Veröffentlichung

2009

Status

Published

Titel

The role of downstream signaling pathways of the epidermal growth factor receptor for artesunate\#8217;s activity in cancer cells

Sub types

Meeting Abstract

Ausgabe der Zeitschrift

69

Data source: Web of Science (Lite)

Autoren

V Konkimalla
James McCubrey
Thomas Efferth

DOI

10.2174/156800909787314020

ISSN

1568-0096

Ausgabe der Veröffentlichung

1

Zeitschrift

Current Cancer Drug Targets

Sprache

en

Paginierung

72 - 80

Datum der Veröffentlichung

2009

Status

Published

Herausgeber

Bentham Science Publishers Ltd.

Herausgeber URL

http://dx.doi.org/10.2174/156800909787314020

Datum der Datenerfassung

2015

Titel

The Role of Downstream Signaling Pathways of the Epidermal Growth Factor Receptor for Artesunates Activity in Cancer Cells

Ausgabe der Zeitschrift

9

Data source: Crossref

Abstract

Epidermal growth factor (EGF) and its receptor (EGFR) as well as the EGFR-coupled Ras>Raf>MEK>ERK pathway are known to affect the survival of cancer cells upon chemotherapeutic treatment. In the present investigation, we analyzed the role of EGFR signaling pathways for the activity of artesunate towards cancer cells. The microarray-based mRNA expression of genes involved in EGFR signaling pathway was correlated with the 50% inhibition concentrations (IC<inf>50</<inf>) of 55 tumor cell lines for artesunate. The log10IC<inf>50</<inf> values were in a range of -6.609 to -4.0M. Candidate genes identified by this approach were then experimentally validated by transfecting cell lines with corresponding cDNA vectors and treating them with artesunate. Indeed, we observed that the Ras>Raf>MEK>ERK pathway is an important signaling route for the response of tumor cells to artesunate. As exemplarily shown for artesunate, the application of such a combined approach to identify signal transduction pathways involved in the response of tumor cells to cytotoxic compounds might foster the development of novel molecular targeted therapies for cancer treatment.

Addresses

German Cancer Research Center, Pharmaceutical Biology, Hei-delberg, Germany. t.efferth@dkfz.de

Autoren

V Badireenath Konkimalla
James A McCubrey
Thomas Efferth
Thomas Efferth

DOI

10.2174/156800909787314020

eISSN

1873-5576

Externe Identifier

PubMed Identifier: 19200051

Open access

false

ISSN

1568-0096

Ausgabe der Veröffentlichung

1

Zeitschrift

Current cancer drug targets

Schlüsselwörter

Cell Line
Humans
Neoplasms
Artemisinins
Signal Transduction
ErbB Receptors
Artesunate

Sprache

eng

Medium

Print

Paginierung

72 - 80

Datum der Veröffentlichung

2009

Status

Published

Datum der Datenerfassung

2009

Titel

The role of downstream signaling pathways of the epidermal growth factor receptor for Artesunate's activity in cancer cells.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

9

Data source: Europe PubMed Central

Abstract

Epidermal growth factor (EGF) and its receptor (EGFR) as well as the EGFR-coupled Ras>Raf>MEK>ERK pathway are known to affect the survival of cancer cells upon chemotherapeutic treatment. In the present investigation, we analyzed the role of EGFR signaling pathways for the activity of artesunate towards cancer cells. The microarray-based mRNA expression of genes involved in EGFR signaling pathway was correlated with the 50% inhibition concentrations (IC<inf>50</<inf>) of 55 tumor cell lines for artesunate. The log10IC<inf>50</<inf> values were in a range of -6.609 to -4.0M. Candidate genes identified by this approach were then experimentally validated by transfecting cell lines with corresponding cDNA vectors and treating them with artesunate. Indeed, we observed that the Ras>Raf>MEK>ERK pathway is an important signaling route for the response of tumor cells to artesunate. As exemplarily shown for artesunate, the application of such a combined approach to identify signal transduction pathways involved in the response of tumor cells to cytotoxic compounds might foster the development of novel molecular targeted therapies for cancer treatment.

Autoren

V Badireenath Konkimalla
James A McCubrey
Thomas Efferth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/19200051

DOI

10.2174/156800909787314020

eISSN

1873-5576

Ausgabe der Veröffentlichung

1

Zeitschrift

Curr Cancer Drug Targets

Schlüsselwörter

Artemisinins
Artesunate
Cell Line
ErbB Receptors
Humans
Neoplasms
Signal Transduction

Sprache

eng

Country

Netherlands

Paginierung

72 - 80

Datum der Veröffentlichung

2009

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2009

Titel

The role of downstream signaling pathways of the epidermal growth factor receptor for Artesunate's activity in cancer cells.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

9

Data source: PubMed

The Role of Downstream Signaling Pathways of the Epidermal Growth Factor Receptor for Artesunate's Activity in Cancer Cells

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