Negative-pressure cavitation extraction for the determination of flavonoids in pigeon pea leaves by liquid chromatography-tandem mass spectrometry

Autoren

Wei Liu
Yujie Fu
Yuangang Zu
Yu Kong
Lin Zhang
Baishi Zu
Thomas Efferth

DOI

10.1016/j.chroma.2009.02.073

ISSN

0021-9673

Ausgabe der Veröffentlichung

18

Zeitschrift

Journal of Chromatography A

Sprache

en

Paginierung

3841 - 3850

Datum der Veröffentlichung

2009

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.chroma.2009.02.073

Datum der Datenerfassung

2022

Titel

Negative-pressure cavitation extraction for the determination of flavonoids in pigeon pea leaves by liquid chromatography–tandem mass spectrometry

Ausgabe der Zeitschrift

1216

Data source: Crossref

Abstract

A new method, namely negative-pressure cavitation extraction (NPCE), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is presented for the extraction and quantification of flavonoids in pigeon pea leaves. This method combines the high efficiency of NPCE and the sensitivity and accuracy of MS/MS. The influential parameters of the NPCE procedure including liquid/solid ratio, extraction time, nitrogen flow and number of extraction cycles were optimized. Under optimized conditions, the efficiency of NPCE for extracting five flavonoids was compared to microwave-assisted extraction (MAE), ultrasonic extraction (USE) and heating reflux extraction (HRE). Additionally, structural disruption to pigeon pea leaves samples with different extraction methods was investigated by scanning electron microscopy. The relative recovery with NPCE was equivalent to or higher than that with USE and obviously higher than those with MAE and HRE which are usually conducted in higher temperatures. Furthermore, because NPCE was performed with nitrogen at room temperature, the degradation and oxidation of analytes were avoided. In addition, the NPCE method was validated in terms of repeatability and reproducibility, relative standard deviation for relative recovery was lower than 5.84 and 8.83%, respectively. The method was also successfully applied for the quantification of five flavonoids in pigeon pea leaves. All these results suggest that the developed NPCE-LC-MS/MS method represents an excellent alternative for the extraction and quantification of flavonoids in other plant materials.

Addresses

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.

Autoren

Wei Liu
Yujie Fu
Yuangang Zu
Yu Kong
Lin Zhang
Baishi Zu
Thomas Efferth
Thomas Efferth

DOI

10.1016/j.chroma.2009.02.073

eISSN

1873-3778

Externe Identifier

PubMed Identifier: 19303077

Open access

false

ISSN

0021-9673

Ausgabe der Veröffentlichung

18

Zeitschrift

Journal of chromatography. A

Schlüsselwörter

Cajanus
Plant Leaves
Flavonoids
Plant Extracts
Chemical Fractionation
Chromatography, Liquid
Tandem Mass Spectrometry

Sprache

eng

Medium

Print-Electronic

Online publication date

2009

Paginierung

3841 - 3850

Datum der Veröffentlichung

2009

Status

Published

Datum der Datenerfassung

2009

Titel

Negative-pressure cavitation extraction for the determination of flavonoids in pigeon pea leaves by liquid chromatography-tandem mass spectrometry.

Sub types

Research Support, Non-U.S. Gov't
Evaluation Study
Journal Article

Ausgabe der Zeitschrift

1216

Data source: Europe PubMed Central

Abstract

A new method, namely negative-pressure cavitation extraction (NPCE), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is presented for the extraction and quantification of flavonoids in pigeon pea leaves. This method combines the high efficiency of NPCE and the sensitivity and accuracy of MS/MS. The influential parameters of the NPCE procedure including liquid/solid ratio, extraction time, nitrogen flow and number of extraction cycles were optimized. Under optimized conditions, the efficiency of NPCE for extracting five flavonoids was compared to microwave-assisted extraction (MAE), ultrasonic extraction (USE) and heating reflux extraction (HRE). Additionally, structural disruption to pigeon pea leaves samples with different extraction methods was investigated by scanning electron microscopy. The relative recovery with NPCE was equivalent to or higher than that with USE and obviously higher than those with MAE and HRE which are usually conducted in higher temperatures. Furthermore, because NPCE was performed with nitrogen at room temperature, the degradation and oxidation of analytes were avoided. In addition, the NPCE method was validated in terms of repeatability and reproducibility, relative standard deviation for relative recovery was lower than 5.84 and 8.83%, respectively. The method was also successfully applied for the quantification of five flavonoids in pigeon pea leaves. All these results suggest that the developed NPCE-LC-MS/MS method represents an excellent alternative for the extraction and quantification of flavonoids in other plant materials.

Date of acceptance

2009

Autoren

Wei Liu
Yujie Fu
Yuangang Zu
Yu Kong
Lin Zhang
Baishi Zu
Thomas Efferth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/19303077

DOI

10.1016/j.chroma.2009.02.073

eISSN

1873-3778

Ausgabe der Veröffentlichung

18

Zeitschrift

J Chromatogr A

Schlüsselwörter

Cajanus
Chemical Fractionation
Chromatography, Liquid
Flavonoids
Plant Extracts
Plant Leaves
Tandem Mass Spectrometry

Sprache

eng

Country

Netherlands

Paginierung

3841 - 3850

PII

S0021-9673(09)00326-4

Datum der Veröffentlichung

2009

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2009

Titel

Negative-pressure cavitation extraction for the determination of flavonoids in pigeon pea leaves by liquid chromatography-tandem mass spectrometry.

Sub types

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

1216

Data source: PubMed

Negative-pressure cavitation extraction for the determination of flavonoids in pigeon pea leaves by liquid chromatography-tandem mass spectrometry

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