Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae)

Publication type:
Journal article
Metadata:
Autoren
  • Mohamed L Ashour
  • Mahmoud El-Readi
  • Mahmoud Youns
  • Sri Mulyaningsih
  • Frank Sporer
  • Thomas Efferth
  • Michael Wink
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000269501200012&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1211/jpp/61.08.0012
eISSN
2042-7158
Externe Identifier
  • Clarivate Analytics Document Solution ID: 490LA
  • PubMed Identifier: 19703352
ISSN
0022-3573
Ausgabe der Veröffentlichung
8
Zeitschrift
JOURNAL OF PHARMACY AND PHARMACOLOGY
Schlüsselwörter
  • anti-inflammatory activity
  • antimicrobial activity
  • Bupleurum marginatum essential oil
  • chemical composition
  • cytotoxicity
Paginierung
1079 - 1087
Datum der Veröffentlichung
2009
Status
Published
Titel
Chemical composition and biological activity of the essential oil obtained from <i>Bupleurum marginatum</i> (Apiaceae)
Sub types
  • Article
Ausgabe der Zeitschrift
61

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Mohamed L Ashour
  • Mahmoud El-Readi
  • Mahmoud Youns
  • Sri Mulyaningsih
  • Frank Sporer
  • Thomas Efferth
  • Michael Wink
DOI
10.1211/jpp/61.08.0012
ISSN
0022-3573
Ausgabe der Veröffentlichung
8
Zeitschrift
Journal of Pharmacy and Pharmacology
Sprache
en
Paginierung
1079 - 1087
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Oxford University Press (OUP)
Herausgeber URL
http://dx.doi.org/10.1211/jpp/61.08.0012
Datum der Datenerfassung
2020
Titel
Chemical composition and biological activity of the essential oil obtained from &lt;I&gt;Bupleurum marginatum&lt;/I&gt; (Apiaceae)
Ausgabe der Zeitschrift
61

Data source: Crossref

Abstract
<h4>Objectives</h4>Bupleurum marginatum is a herb indigenous to the southern and southwestern part of China. It is widely used in many Chinese prescriptions. We aimed to investigate the chemical composition, antioxidant, anti-inflammatory, antimicrobial and in-vitro cytotoxic activity of the hydrodistilled and extracted essential oil from B. marginatum to validate some of its ethnopharmacologial uses.<h4>Methods</h4>The essential oil of the aerial parts of B. marginatum was analysed by capillary gas chromatography (GLC/FID) and gas chromatography-mass spectrometry (GLC/MS). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH(.)) and to prevent the degradation of deoxyribose were used to evaluate the antioxidant activity. Inhibition of both prostaglandin E(2) production and lipoxygenase were used to assess the anti-inflammatory activity. Antimicrobial activity was studied in vitro against a range of bacteria and fungi. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, Caco-2, CCRF-CEM, HeLa, MiaPaCa-2 and MCF-7) was examined using the MTT assay.<h4>Key findings</h4>Seventy-two components, comprising almost 94.29% of the total peak area, were identified in the analysis. The main components were tridecane (13.18%), undecane (10.42%), pentadecane (8.71%), beta-caryophyllene (5.53%) and beta-caryophyllene oxide (5.29%). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH(.)) and to prevent the degradation of deoxyribose were used to evaluate the antioxidant activity and the corresponding IC50 values (drug concentration which resulted in a 50% reduction in inhibition of the activity) were found to be 3.66 mg/ml and 17.4 microg/ml, respectively. Inhibition of both prostaglandin E(2) production and lipoxygenase were used to assess the anti-inflammatory activity (IC50 of 63.64 microg/ml for lipoxygenase, 26.04% inhibition of prostaglandin E(2) at 25 microg/ml dose). The oil also showed a significant in-vitro antimicrobial activity against Gram positive pathogens (Streptococcus pyogenes and Streptococcus agalactiae) with minimum inhibitory concentration (MIC) values ranging from 0.125 up to 4.00 mg/ml. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, Caco-2, CCRF-CEM, HeLa, MiaPaCa-2 and MCF-7) examined using the MTT assay revealed the highest activity to be in the CCRF-CEM cell line with an IC50 (concentration which resulted in a 50% reduction in cell viability) of 46.01 microg/ml after 24 h treatment.<h4>Conclusions</h4>The essential oil of B. marginatum exhibited a promising anti-inflammatory activity along with strong cytotoxicity against many cancer cells (CCRF-CEM and HepG2) mediated through induction of apoptosis, and this in-vitro activity make its local traditional uses rational. However, its limited antimicrobial activity indicates that a combination with other drugs is essential for effective use. Further selectivity testing is required to evaluate the effect of the oil against normal cells.
Addresses
  • Institute of Pharmacy and Molecular Biotechnology, University Heidelberg, Heidelberg, Germany.
Autoren
  • Mohamed L Ashour
  • Mahmoud El-Readi
  • Mahmoud Youns
  • Sri Mulyaningsih
  • Frank Sporer
  • Thomas Efferth
  • Thomas Efferth
  • Michael Wink
DOI
10.1211/jpp/61.08.0012
eISSN
2042-7158
Externe Identifier
  • PubMed Identifier: 19703352
Open access
false
ISSN
0022-3573
Ausgabe der Veröffentlichung
8
Zeitschrift
The Journal of pharmacy and pharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Bupleurum
  • Plant Components, Aerial
  • Oils, Volatile
  • Anti-Inflammatory Agents
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Anti-Infective Agents
  • Medicine, Chinese Traditional
  • Drug Screening Assays, Antitumor
  • Apoptosis
Sprache
eng
Medium
Print
Paginierung
1079 - 1087
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2009
Titel
Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae).
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
61

Data source: Europe PubMed Central

Abstract
OBJECTIVES: Bupleurum marginatum is a herb indigenous to the southern and southwestern part of China. It is widely used in many Chinese prescriptions. We aimed to investigate the chemical composition, antioxidant, anti-inflammatory, antimicrobial and in-vitro cytotoxic activity of the hydrodistilled and extracted essential oil from B. marginatum to validate some of its ethnopharmacologial uses. METHODS: The essential oil of the aerial parts of B. marginatum was analysed by capillary gas chromatography (GLC/FID) and gas chromatography-mass spectrometry (GLC/MS). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH(.)) and to prevent the degradation of deoxyribose were used to evaluate the antioxidant activity. Inhibition of both prostaglandin E(2) production and lipoxygenase were used to assess the anti-inflammatory activity. Antimicrobial activity was studied in vitro against a range of bacteria and fungi. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, Caco-2, CCRF-CEM, HeLa, MiaPaCa-2 and MCF-7) was examined using the MTT assay. KEY FINDINGS: Seventy-two components, comprising almost 94.29% of the total peak area, were identified in the analysis. The main components were tridecane (13.18%), undecane (10.42%), pentadecane (8.71%), beta-caryophyllene (5.53%) and beta-caryophyllene oxide (5.29%). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH(.)) and to prevent the degradation of deoxyribose were used to evaluate the antioxidant activity and the corresponding IC50 values (drug concentration which resulted in a 50% reduction in inhibition of the activity) were found to be 3.66 mg/ml and 17.4 microg/ml, respectively. Inhibition of both prostaglandin E(2) production and lipoxygenase were used to assess the anti-inflammatory activity (IC50 of 63.64 microg/ml for lipoxygenase, 26.04% inhibition of prostaglandin E(2) at 25 microg/ml dose). The oil also showed a significant in-vitro antimicrobial activity against Gram positive pathogens (Streptococcus pyogenes and Streptococcus agalactiae) with minimum inhibitory concentration (MIC) values ranging from 0.125 up to 4.00 mg/ml. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, Caco-2, CCRF-CEM, HeLa, MiaPaCa-2 and MCF-7) examined using the MTT assay revealed the highest activity to be in the CCRF-CEM cell line with an IC50 (concentration which resulted in a 50% reduction in cell viability) of 46.01 microg/ml after 24 h treatment. CONCLUSIONS: The essential oil of B. marginatum exhibited a promising anti-inflammatory activity along with strong cytotoxicity against many cancer cells (CCRF-CEM and HepG2) mediated through induction of apoptosis, and this in-vitro activity make its local traditional uses rational. However, its limited antimicrobial activity indicates that a combination with other drugs is essential for effective use. Further selectivity testing is required to evaluate the effect of the oil against normal cells.
Autoren
  • Mohamed L Ashour
  • Mahmoud El-Readi
  • Mahmoud Youns
  • Sri Mulyaningsih
  • Frank Sporer
  • Thomas Efferth
  • Michael Wink
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19703352
DOI
10.1211/jpp/61.08.0012
ISSN
0022-3573
Ausgabe der Veröffentlichung
8
Zeitschrift
J Pharm Pharmacol
Schlüsselwörter
  • Anti-Infective Agents
  • Anti-Inflammatory Agents
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Apoptosis
  • Bupleurum
  • Cell Line, Tumor
  • Drug Screening Assays, Antitumor
  • Humans
  • Medicine, Chinese Traditional
  • Oils, Volatile
  • Plant Components, Aerial
  • Plant Extracts
Sprache
eng
Country
England
Paginierung
1079 - 1087
Datum der Veröffentlichung
2009
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae).
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
61

Data source: PubMed

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