Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS

Publication type:
Journal article
Metadata:
Autoren
  • Shuangming Li
  • Yujie Fu
  • Yuangang Zu
  • Baishi Zu
  • Ying Wang
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000273993500014&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/jssc.200900402
eISSN
1615-9314
Externe Identifier
  • Clarivate Analytics Document Solution ID: 548UN
  • PubMed Identifier: 19839015
ISSN
1615-9306
Ausgabe der Veröffentlichung
22
Zeitschrift
JOURNAL OF SEPARATION SCIENCE
Schlüsselwörter
  • Determination
  • Negative pressure cavitation (NPC)
  • Paclitaxel
  • Separation
  • Taxoids
Paginierung
3958 - 3966
Datum der Veröffentlichung
2009
Status
Published
Titel
Determination of paclitaxel and its analogues in the needles of <i>Taxus</i> species by using negative pressure cavitation extraction followed by HPLC-MS-MS
Sub types
  • Article
Ausgabe der Zeitschrift
32

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:p>A new separation method, negative pressure cavitation (NPC) extraction followed by HPLC‐MS‐MS for the determination of paclitaxel and its analogues in the needles of <jats:italic>Taxus</jats:italic> species is described in this study. Compared with three conventional extraction methods, NPC is a more effective, economical, and facile method for the separation of nature compounds from herbal plants. In contrast to high‐temperature extraction, NPC at low temperature can minimize undesirable reactions such as thermal decomposition or degradation. In addition, the extraction mechanism of NPC was also illuminated. The effects of vacuum degree, extraction solvent, ratio of solid to liquid, time, and times extraction were studied and optimized. The optimum extraction conditions were as follows: vacuum degree –0.03 MPa, solvent 80% v/v alcohol, ratio of material to liquid 1:15 (g/mL), extraction time 60 min, extraction (×3). Using these conditions, the recoveries of 10‐deacetyl‐7‐xylosylpaclitaxel, 10‐deacetylpaclitaxel, cephalomannine, and paclitaxel were higher than 95.88, 95.82, 94.85, and 96.18%, respectively. The contents of paclitaxel, 10‐deacetyl‐7‐xylosylpaclitaxel, 10‐deacetylpaclitaxel, and cephalomannine for <jats:italic>Taxus chinensis</jats:italic> were 0.0053, 0.0467, 0.0132, and 0.0076%, and 0.0067, 0.0153, 0.0047, and 0.0064% for <jats:italic>Taxus cuspidata</jats:italic>, respectively. Furthermore, a comparison of SEM images observed the morphological changes of microstructures and cellular damage in yew needles.</jats:p>
Autoren
  • Shuangming Li
  • Yujie Fu
  • Yuangang Zu
  • Baishi Zu
  • Ying Wang
  • Thomas Efferth
DOI
10.1002/jssc.200900402
eISSN
1615-9314
ISSN
1615-9306
Ausgabe der Veröffentlichung
22
Zeitschrift
Journal of Separation Science
Sprache
en
Online publication date
2009
Paginierung
3958 - 3966
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/jssc.200900402
Datum der Datenerfassung
2023
Titel
Determination of paclitaxel and its analogues in the needles of <i>Taxus</i> species by using negative pressure cavitation extraction followed by HPLC‐MS‐MS
Ausgabe der Zeitschrift
32

Data source: Crossref

Abstract
A new separation method, negative pressure cavitation (NPC) extraction followed by HPLC-MS-MS for the determination of paclitaxel and its analogues in the needles of Taxus species is described in this study. Compared with three conventional extraction methods, NPC is a more effective, economical, and facile method for the separation of nature compounds from herbal plants. In contrast to high-temperature extraction, NPC at low temperature can minimize undesirable reactions such as thermal decomposition or degradation. In addition, the extraction mechanism of NPC was also illuminated. The effects of vacuum degree, extraction solvent, ratio of solid to liquid, time, and times extraction were studied and optimized. The optimum extraction conditions were as follows: vacuum degree -0.03 MPa, solvent 80% v/v alcohol, ratio of material to liquid 1:15 (g/mL), extraction time 60 min, extraction (x3). Using these conditions, the recoveries of 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, cephalomannine, and paclitaxel were higher than 95.88, 95.82, 94.85, and 96.18%, respectively. The contents of paclitaxel, 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, and cephalomannine for Taxus chinensis were 0.0053, 0.0467, 0.0132, and 0.0076%, and 0.0067, 0.0153, 0.0047, and 0.0064% for Taxus cuspidata, respectively. Furthermore, a comparison of SEM images observed the morphological changes of microstructures and cellular damage in yew needles.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.
Autoren
  • Shuangming Li
  • Yujie Fu
  • Yuangang Zu
  • Baishi Zu
  • Ying Wang
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1002/jssc.200900402
eISSN
1615-9314
Externe Identifier
  • PubMed Identifier: 19839015
Open access
false
ISSN
1615-9306
Ausgabe der Veröffentlichung
22
Zeitschrift
Journal of separation science
Schlüsselwörter
  • Taxus
  • Paclitaxel
  • Antineoplastic Agents, Phytogenic
  • Microscopy, Electron, Scanning
  • Chromatography, High Pressure Liquid
  • Pressure
  • Reference Standards
  • Tandem Mass Spectrometry
Sprache
eng
Medium
Print
Paginierung
3958 - 3966
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2009
Titel
Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS.
Sub types
  • Research Support, Non-U.S. Gov't
  • Validation Study
  • Journal Article
Ausgabe der Zeitschrift
32

Data source: Europe PubMed Central

Abstract
A new separation method, negative pressure cavitation (NPC) extraction followed by HPLC-MS-MS for the determination of paclitaxel and its analogues in the needles of Taxus species is described in this study. Compared with three conventional extraction methods, NPC is a more effective, economical, and facile method for the separation of nature compounds from herbal plants. In contrast to high-temperature extraction, NPC at low temperature can minimize undesirable reactions such as thermal decomposition or degradation. In addition, the extraction mechanism of NPC was also illuminated. The effects of vacuum degree, extraction solvent, ratio of solid to liquid, time, and times extraction were studied and optimized. The optimum extraction conditions were as follows: vacuum degree -0.03 MPa, solvent 80% v/v alcohol, ratio of material to liquid 1:15 (g/mL), extraction time 60 min, extraction (x3). Using these conditions, the recoveries of 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, cephalomannine, and paclitaxel were higher than 95.88, 95.82, 94.85, and 96.18%, respectively. The contents of paclitaxel, 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, and cephalomannine for Taxus chinensis were 0.0053, 0.0467, 0.0132, and 0.0076%, and 0.0067, 0.0153, 0.0047, and 0.0064% for Taxus cuspidata, respectively. Furthermore, a comparison of SEM images observed the morphological changes of microstructures and cellular damage in yew needles.
Autoren
  • Shuangming Li
  • Yujie Fu
  • Yuangang Zu
  • Baishi Zu
  • Ying Wang
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19839015
DOI
10.1002/jssc.200900402
eISSN
1615-9314
Ausgabe der Veröffentlichung
22
Zeitschrift
J Sep Sci
Schlüsselwörter
  • Antineoplastic Agents, Phytogenic
  • Chromatography, High Pressure Liquid
  • Microscopy, Electron, Scanning
  • Paclitaxel
  • Pressure
  • Reference Standards
  • Tandem Mass Spectrometry
  • Taxus
Sprache
eng
Country
Germany
Paginierung
3958 - 3966
Datum der Veröffentlichung
2009
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Validation Study
Ausgabe der Zeitschrift
32

Data source: PubMed

Beziehungen:
Property of

Tools