Cytotoxic Activity of Curcumin towards CCRF-CEM Leukemia Cells and Its Effect on DNA Damage
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Yu Kong
- Wei Ma
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Nan Wu
- Lu Liang
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000273043200037&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/molecules14125328
- Externe Identifier
- Clarivate Analytics Document Solution ID: 536KL
- PubMed Identifier: 20032896
- ISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- MOLECULES
- Schlüsselwörter
- curcumin
- activity
- CEM
- DNA damage
- Paginierung
- 5328 - 5338
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- Cytotoxic Activity of Curcumin towards CCRF-CEM Leukemia Cells and Its Effect on DNA Damage
- Sub types
- Article
- Ausgabe der Zeitschrift
- 14
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Yu Kong
- Wei Ma
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Nan Wu
- Lu Liang
- Liping Yao
- Thomas Efferth
- DOI
- 10.3390/molecules14125328
- eISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- Molecules
- Sprache
- en
- Online publication date
- 2009
- Paginierung
- 5328 - 5338
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/molecules14125328
- Datum der Datenerfassung
- 2018
- Titel
- Cytotoxic Activity of Curcumin towards CCRF-CEM Leukemia Cells and Its Effect on DNA Damage
- Ausgabe der Zeitschrift
- 14
Data source: Crossref
- Abstract
- The cytotoxic activity of curcumin towards CCRF-CEM human T-cell leukemia cells was measured by the MTT assay. Tumor cells were more sensitive to the cytotoxic activity of curcumin or curcumin-Cu (II)compared to normal cells, and the IC(50) of curcumin towards CCRF-CEM cells was 8.68 microM, and that of curcumin-Cu (II) was 8.14 microM. The cell cycle distribution of curcumin-treated CCRF-CEM cells was analyzed by flow cytometry. DNA damage induced by oxidants such as curcumin-Cu (II) ions is considered as one of the main causes of cell inactivation. Therefore, we analyzed the effect of curcumin on DNA damage by agarose gel electrophoresis and atomic force microscopy (AFM). Gel electrophoresis analyses showed that curcumin or Cu (II) alone failed to cause DNA damage in pBR322 plasmid DNA as compared with the normal plasmid. However, DNA plasmids were mostly damaged after treatment with curcumin of different concentrations in the presence of Cu (II). Two forms were observed by means of AFM: closed circular plasmids and linear plasmids. DNA damage induced by a combination of curcumin and Cu (II) was also found by agarose gel electrophoresis, which was applied as control method to verify the results obtained by AFM.
- Addresses
- Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. kongyu820202@yahoo.com.cn
- Autoren
- Yu Kong
- Wei Ma
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Nan Wu
- Lu Liang
- Liping Yao
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.3390/molecules14125328
- eISSN
- 1420-3049
- Externe Identifier
- PubMed Identifier: 20032896
- PubMed Central ID: PMC6255027
- Open access
- true
- ISSN
- 1420-3049
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- Molecules (Basel, Switzerland)
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Leukemia, T-Cell
- DNA Damage
- Curcumin
- Antineoplastic Agents
- Microscopy, Atomic Force
- Electrophoresis, Agar Gel
- Cell Cycle
- Plasmids
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2009
- Open access status
- Open Access
- Paginierung
- 5328 - 5338
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2009
- Titel
- Cytotoxic activity of curcumin towards CCRF-CEM leukemia cells and its effect on DNA damage.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 14
Files
https://www.mdpi.com/1420-3049/14/12/5328/pdf?version=1403112655 https://europepmc.org/articles/PMC6255027?pdf=render
Data source: Europe PubMed Central
- Abstract
- The cytotoxic activity of curcumin towards CCRF-CEM human T-cell leukemia cells was measured by the MTT assay. Tumor cells were more sensitive to the cytotoxic activity of curcumin or curcumin-Cu (II)compared to normal cells, and the IC(50) of curcumin towards CCRF-CEM cells was 8.68 microM, and that of curcumin-Cu (II) was 8.14 microM. The cell cycle distribution of curcumin-treated CCRF-CEM cells was analyzed by flow cytometry. DNA damage induced by oxidants such as curcumin-Cu (II) ions is considered as one of the main causes of cell inactivation. Therefore, we analyzed the effect of curcumin on DNA damage by agarose gel electrophoresis and atomic force microscopy (AFM). Gel electrophoresis analyses showed that curcumin or Cu (II) alone failed to cause DNA damage in pBR322 plasmid DNA as compared with the normal plasmid. However, DNA plasmids were mostly damaged after treatment with curcumin of different concentrations in the presence of Cu (II). Two forms were observed by means of AFM: closed circular plasmids and linear plasmids. DNA damage induced by a combination of curcumin and Cu (II) was also found by agarose gel electrophoresis, which was applied as control method to verify the results obtained by AFM.
- Date of acceptance
- 2009
- Autoren
- Yu Kong
- Wei Ma
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Nan Wu
- Lu Liang
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20032896
- DOI
- 10.3390/molecules14125328
- eISSN
- 1420-3049
- Externe Identifier
- PubMed Central ID: PMC6255027
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- Molecules
- Schlüsselwörter
- Antineoplastic Agents
- Cell Cycle
- Cell Line, Tumor
- Curcumin
- DNA Damage
- Electrophoresis, Agar Gel
- Humans
- Leukemia, T-Cell
- Microscopy, Atomic Force
- Plasmids
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 5328 - 5338
- PII
- molecules14125328
- Datum der Veröffentlichung
- 2009
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Cytotoxic activity of curcumin towards CCRF-CEM leukemia cells and its effect on DNA damage.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 14
Data source: PubMed
- Beziehungen:
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