Daedalea gibbosa substances inhibit LPS-induced expression of NOS by suppression of NF-κB and MAPK activities in RAW 264.7 macrophage cells

Autoren

Mahajna

DOI

10.3892/ijmm_00000361

eISSN

1791-244X

ISSN

1107-3756

Ausgabe der Veröffentlichung

3

Zeitschrift

International Journal of Molecular Medicine

Datum der Veröffentlichung

2010

Status

Published

Herausgeber

Spandidos Publications

Herausgeber URL

http://dx.doi.org/10.3892/ijmm_00000361

Datum der Datenerfassung

2017

Titel

Daedalea gibbosa substances inhibit LPS-induced expression of iNOS by suppression of NF-κB and MAPK activities in RAW 264.7 macrophage cells

Ausgabe der Zeitschrift

25

Data source: Crossref

Abstract

Nitric oxide (NO) is a radical molecule produced by iNOS and plays a role in various physiological and pathophysiological conditions including inflammatory diseases and cancer. In the present study, organic extract of Daedalea gibbosa was effective in inhibiting NO and PGE2 production in RAW 264.7 cells. The extract of D. gibbosa was chemically fractionated leading to the isolation of three active fractions (F5-F7) that were effective in inhibiting NO and iNOS production. In addition, F6 and F7 significantly inhibited the iNOS transcript, while F5 did not cause a reduction in the iNOS transcript. Furthermore, the active fractions showed a differential effect on levels of phospho-p38, phospho-JNK, and phospho-IKBalpha. Phopsho-p38 was moderately inhibited by F5 and only F7 was significantly active in inhibiting phospho-IKBalpha. Interestingly, all active fractions significantly enhanced levels of phospho-JNK. In addition, the three active fractions also showed differential inhibitory effects on NF-kappaB DNA binding activity.

Addresses

Cancer Drug Discovery Program, Migal, Galilee Technology Center, Kiryat Shmona, Israel.

Autoren

Nili Ruimi
Haithem Rwashdeh
Solomon Wasser
Badireenath Konkimalla
Thomas Efferth
Thomas Efferth
Monica Borgatti
Roberto Gambari
Jamal Mahajna

DOI

10.3892/ijmm_00000361

eISSN

1791-244X

Externe Identifier

PubMed Identifier: 20127048

Open access

false

ISSN

1107-3756

Ausgabe der Veröffentlichung

3

Zeitschrift

International journal of molecular medicine

Schlüsselwörter

Cell Line
Macrophages
Animals
Humans
Mice
Agaricales
Nitric Oxide
Mitogen-Activated Protein Kinases
JNK Mitogen-Activated Protein Kinases
Lipopolysaccharides
Dinoprostone
NF-kappa B
Microarray Analysis
Nitric Oxide Synthase Type II
Promoter Regions, Genetic

Sprache

eng

Medium

Print

Paginierung

421 - 432

Datum der Veröffentlichung

2010

Status

Published

Datum der Datenerfassung

2010

Titel

Daedalea gibbosa substances inhibit LPS-induced expression of iNOS by suppression of NF-kappaB and MAPK activities in RAW 264.7 macrophage cells.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

25

Data source: Europe PubMed Central

Abstract

Nitric oxide (NO) is a radical molecule produced by iNOS and plays a role in various physiological and pathophysiological conditions including inflammatory diseases and cancer. In the present study, organic extract of Daedalea gibbosa was effective in inhibiting NO and PGE2 production in RAW 264.7 cells. The extract of D. gibbosa was chemically fractionated leading to the isolation of three active fractions (F5-F7) that were effective in inhibiting NO and iNOS production. In addition, F6 and F7 significantly inhibited the iNOS transcript, while F5 did not cause a reduction in the iNOS transcript. Furthermore, the active fractions showed a differential effect on levels of phospho-p38, phospho-JNK, and phospho-IKBalpha. Phopsho-p38 was moderately inhibited by F5 and only F7 was significantly active in inhibiting phospho-IKBalpha. Interestingly, all active fractions significantly enhanced levels of phospho-JNK. In addition, the three active fractions also showed differential inhibitory effects on NF-kappaB DNA binding activity.

Autoren

Nili Ruimi
Haithem Rwashdeh
Solomon Wasser
Badireenath Konkimalla
Thomas Efferth
Monica Borgatti
Roberto Gambari
Jamal Mahajna

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/20127048

DOI

10.3892/ijmm_00000361

eISSN

1791-244X

Ausgabe der Veröffentlichung

3

Zeitschrift

Int J Mol Med

Schlüsselwörter

Agaricales
Animals
Cell Line
Dinoprostone
Humans
JNK Mitogen-Activated Protein Kinases
Lipopolysaccharides
Macrophages
Mice
Microarray Analysis
Mitogen-Activated Protein Kinases
NF-kappa B
Nitric Oxide
Nitric Oxide Synthase Type II
Promoter Regions, Genetic

Sprache

eng

Country

Greece

Paginierung

421 - 432

Datum der Veröffentlichung

2010

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2010

Titel

Daedalea gibbosa substances inhibit LPS-induced expression of iNOS by suppression of NF-kappaB and MAPK activities in RAW 264.7 macrophage cells.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

25

Data source: PubMed

Daedalea gibbosa substances inhibit LPS-induced expression of NOS by suppression of NF-κB and MAPK activities in RAW 264.7 macrophage cells

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