Plasma pharmacokinetics and tissue distribution study of cajaninstilbene acid in rats by liquid chromatography with tandem mass spectrometry
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Xin Hua
- Yu-Jie Fu
- Yuan-Gang Zu
- Nan Wu
- Yu Kong
- Ji Li
- Xiao Peng
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000274979700016&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.jpba.2010.01.004
- eISSN
- 1873-264X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 561LV
- PubMed Identifier: 20116956
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
- Schlüsselwörter
- Cajanus cajan
- Cajaninstilbene acid (CSA)
- LC-MS/MS
- Pharmacokinetics
- Tissue distribution
- Paginierung
- 273 - 279
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Plasma pharmacokinetics and tissue distribution study of cajaninstilbene acid in rats by liquid chromatography with tandem mass spectrometry
- Sub types
- Article
- Ausgabe der Zeitschrift
- 52
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Xin Hua
- Yu-Jie Fu
- Yuan-Gang Zu
- Nan Wu
- Yu Kong
- Ji Li
- Xiao Peng
- Thomas Efferth
- DOI
- 10.1016/j.jpba.2010.01.004
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Journal of Pharmaceutical and Biomedical Analysis
- Sprache
- en
- Paginierung
- 273 - 279
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.jpba.2010.01.004
- Datum der Datenerfassung
- 2018
- Titel
- Plasma pharmacokinetics and tissue distribution study of cajaninstilbene acid in rats by liquid chromatography with tandem mass spectrometry
- Ausgabe der Zeitschrift
- 52
Data source: Crossref
- Abstract
- Cajaninstilbene acid (CSA; 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) is a major active constituent of pigeonpea leaves, has been proven to be effective in clinical treatment of diabetes, hepatitis, measles and dysentery. A rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of CSA in rat plasma and various tissues (brain, heart, lung, liver, spleen, small intestine and kidney) of rat for the first time. Rat plasma and tissue distribution pre-treated by protein precipitation with acetoacetate was analyzed using LC-MS/MS with an electrospray ionization (ESI) interface, and isoliquiritigenin was used as an internal standard. Chromatographic separation was achieved on a HIQ Sil C(18) column with the mobile phase of water and methanol (9:91, v/v) containing 0.1% formic acid and resulted in a total run time of 10 min. The isocratic elution mode pumped at a flow rate of 1.0 mL/min. The lower limit of quantification (LLOQ) which was 10 ng/mL. The calibration curve was linear from 10 to 6000 ng/mL (R=0.9967) for plasma samples and 10 to 6000 ng/mL (R>or=0.9974) for tissue homogenates. The intra- and inter-day assay of precision in plasma and tissues ranged from 0.6% to 6.1% and 1.5% to 6.6%, respectively, and the intra- and inter-day assay accuracy was 93.5-104.6% and 93.3-107.5%, respectively. Recoveries in plasma and tissues ranged from 95.0% to 106.8%. The method was successfully applied in pharmacokinetic and tissue distribution studies of CSA after oral administration to rats. The pharmacokinetics of CSA showed rapid absorption and elimination (T(max), 10.7+/-0.31 min; t(1/2), 51.40+/-6.54 min). After oral administration in rats, CSA was rapidly and widely distributed in tissues. High concentrations were found in liver and kidney indicating that CSA was possibly absorbed by liver and eliminated by kidney.
- Addresses
- Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
- Autoren
- Xin Hua
- Yu-Jie Fu
- Yuan-Gang Zu
- Nan Wu
- Yu Kong
- Ji Li
- Xiao Peng
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.jpba.2010.01.004
- eISSN
- 1873-264X
- Externe Identifier
- PubMed Identifier: 20116956
- Open access
- false
- ISSN
- 0731-7085
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Journal of pharmaceutical and biomedical analysis
- Schlüsselwörter
- Animals
- Rats, Inbred Strains
- Rats
- Cajanus
- Carboxylic Acids
- Salicylates
- Stilbenes
- Metabolic Clearance Rate
- Calibration
- Chromatography, Liquid
- Area Under Curve
- Sensitivity and Specificity
- Reproducibility of Results
- Drug Stability
- Temperature
- Molecular Structure
- Tissue Distribution
- Freezing
- Half-Life
- Time Factors
- Reference Standards
- Quality Control
- Female
- Tandem Mass Spectrometry
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2010
- Paginierung
- 273 - 279
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum der Datenerfassung
- 2010
- Titel
- Plasma pharmacokinetics and tissue distribution study of cajaninstilbene acid in rats by liquid chromatography with tandem mass spectrometry.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 52
Data source: Europe PubMed Central
- Abstract
- Cajaninstilbene acid (CSA; 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) is a major active constituent of pigeonpea leaves, has been proven to be effective in clinical treatment of diabetes, hepatitis, measles and dysentery. A rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of CSA in rat plasma and various tissues (brain, heart, lung, liver, spleen, small intestine and kidney) of rat for the first time. Rat plasma and tissue distribution pre-treated by protein precipitation with acetoacetate was analyzed using LC-MS/MS with an electrospray ionization (ESI) interface, and isoliquiritigenin was used as an internal standard. Chromatographic separation was achieved on a HIQ Sil C(18) column with the mobile phase of water and methanol (9:91, v/v) containing 0.1% formic acid and resulted in a total run time of 10 min. The isocratic elution mode pumped at a flow rate of 1.0 mL/min. The lower limit of quantification (LLOQ) which was 10 ng/mL. The calibration curve was linear from 10 to 6000 ng/mL (R=0.9967) for plasma samples and 10 to 6000 ng/mL (R>or=0.9974) for tissue homogenates. The intra- and inter-day assay of precision in plasma and tissues ranged from 0.6% to 6.1% and 1.5% to 6.6%, respectively, and the intra- and inter-day assay accuracy was 93.5-104.6% and 93.3-107.5%, respectively. Recoveries in plasma and tissues ranged from 95.0% to 106.8%. The method was successfully applied in pharmacokinetic and tissue distribution studies of CSA after oral administration to rats. The pharmacokinetics of CSA showed rapid absorption and elimination (T(max), 10.7+/-0.31 min; t(1/2), 51.40+/-6.54 min). After oral administration in rats, CSA was rapidly and widely distributed in tissues. High concentrations were found in liver and kidney indicating that CSA was possibly absorbed by liver and eliminated by kidney.
- Date of acceptance
- 2010
- Autoren
- Xin Hua
- Yu-Jie Fu
- Yuan-Gang Zu
- Nan Wu
- Yu Kong
- Ji Li
- Xiao Peng
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20116956
- DOI
- 10.1016/j.jpba.2010.01.004
- eISSN
- 1873-264X
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- J Pharm Biomed Anal
- Schlüsselwörter
- Animals
- Area Under Curve
- Cajanus
- Calibration
- Carboxylic Acids
- Chromatography, Liquid
- Drug Stability
- Female
- Freezing
- Half-Life
- Metabolic Clearance Rate
- Molecular Structure
- Quality Control
- Rats
- Rats, Inbred Strains
- Reference Standards
- Reproducibility of Results
- Salicylates
- Sensitivity and Specificity
- Stilbenes
- Tandem Mass Spectrometry
- Temperature
- Time Factors
- Tissue Distribution
- Sprache
- eng
- Country
- England
- Paginierung
- 273 - 279
- PII
- S0731-7085(10)00007-5
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Plasma pharmacokinetics and tissue distribution study of cajaninstilbene acid in rats by liquid chromatography with tandem mass spectrometry.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 52
Data source: PubMed
- Beziehungen:
- Property of