A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner

Publication type:
Journal article
Metadata:
Autoren
  • Beate Rinner
  • Nadine Kretschmer
  • Heike Knausz
  • Andrea Mayer
  • Herbert Boechzelt
  • Xiao-Jiang Hao
  • Guenther Heubl
  • Thomas Efferth
  • Helmut Schaider
  • Rudolf Bauer
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000278651300005&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2010.02.006
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: 609JA
  • PubMed Identifier: 20226848
ISSN
0378-8741
Ausgabe der Veröffentlichung
2
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Onosma paniculatum
  • Zicao
  • Cancer
  • Cell cycle
  • Caspase-3
Paginierung
182 - 188
Datum der Veröffentlichung
2010
Status
Published
Titel
A petrol ether extract of the roots of <i>Onosma paniculatum</i> induces cell death in a caspase dependent manner
Sub types
  • Article
Ausgabe der Zeitschrift
129

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Beate Rinner
  • Nadine Kretschmer
  • Heike Knausz
  • Andrea Mayer
  • Herbert Boechzelt
  • Xiao-Jiang Hao
  • Guenther Heubl
  • Thomas Efferth
  • Helmut Schaider
  • Rudolf Bauer
DOI
10.1016/j.jep.2010.02.006
ISSN
0378-8741
Ausgabe der Veröffentlichung
2
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
182 - 188
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2010.02.006
Datum der Datenerfassung
2018
Titel
A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner
Ausgabe der Zeitschrift
129

Data source: Crossref

Abstract
<h4>Aim of the study</h4>Traditional Chinese medicine (TCM) has become very popular in Western countries during the last years. Zicao, a remedy of TCM, has been traditionally used to treat cancer, and, its main constituents, naphthoquinones, have been reported to possess antitumor activity (Chen et al., 2002; Papageorgiou et al., 1999). Here, we prepared extracts of different polarities of Onosma paniculatum Bur. & Franch., a plant which is amongst others used as Zicao, but, much less investigated. The extracts were analyzed concerning their growth inhibitory and apoptosis-inducing activity in various tumor cells.<h4>Materials and methods</h4>Cell viability was measured by XTT viability and a growth inhibition assay. Effects on the cell cycle and caspase-3 were determined by flow cytometry.<h4>Results</h4>From three different extracts, a petrol ether extract showed significant growth inhibitory effect, cell cycle influence and caspase-3 dependent induction of apoptosis which was time and dose dependent.<h4>Conclusion</h4>To further determine the activity and mechanism of action of the petrol ether extract, we would like to isolate and identify the active principle and investigate the effects in more detail.
Addresses
  • Center of Medical Research, Core Facility Flow Cytometry, Medical University of Graz, Graz, Austria. Beate.Rinner@klinikum-graz.at
Autoren
  • Beate Rinner
  • Nadine Kretschmer
  • Heike Knausz
  • Andrea Mayer
  • Herbert Boechzelt
  • Xiao-Jiang Hao
  • Guenther Heubl
  • Thomas Efferth
  • Thomas Efferth
  • Helmut Schaider
  • Rudolf Bauer
DOI
10.1016/j.jep.2010.02.006
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 20226848
Funding acknowledgements
  • Austrian Science Fund FWF: P 21114
Open access
false
ISSN
0378-8741
Ausgabe der Veröffentlichung
2
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Boraginaceae
  • Plant Roots
  • Neoplasms
  • Drugs, Chinese Herbal
  • Antineoplastic Agents, Phytogenic
  • Phytotherapy
  • Cell Cycle
  • Apoptosis
  • Cell Proliferation
  • Dose-Response Relationship, Drug
  • Caspase 3
  • Ether
Sprache
eng
Medium
Print-Electronic
Online publication date
2010
Paginierung
182 - 188
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
129

Data source: Europe PubMed Central

Abstract
AIM OF THE STUDY: Traditional Chinese medicine (TCM) has become very popular in Western countries during the last years. Zicao, a remedy of TCM, has been traditionally used to treat cancer, and, its main constituents, naphthoquinones, have been reported to possess antitumor activity (Chen et al., 2002; Papageorgiou et al., 1999). Here, we prepared extracts of different polarities of Onosma paniculatum Bur. & Franch., a plant which is amongst others used as Zicao, but, much less investigated. The extracts were analyzed concerning their growth inhibitory and apoptosis-inducing activity in various tumor cells. MATERIALS AND METHODS: Cell viability was measured by XTT viability and a growth inhibition assay. Effects on the cell cycle and caspase-3 were determined by flow cytometry. RESULTS: From three different extracts, a petrol ether extract showed significant growth inhibitory effect, cell cycle influence and caspase-3 dependent induction of apoptosis which was time and dose dependent. CONCLUSION: To further determine the activity and mechanism of action of the petrol ether extract, we would like to isolate and identify the active principle and investigate the effects in more detail.
Date of acceptance
2010
Autoren
  • Beate Rinner
  • Nadine Kretschmer
  • Heike Knausz
  • Andrea Mayer
  • Herbert Boechzelt
  • Xiao-Jiang Hao
  • Guenther Heubl
  • Thomas Efferth
  • Helmut Schaider
  • Rudolf Bauer
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20226848
DOI
10.1016/j.jep.2010.02.006
eISSN
1872-7573
Funding acknowledgements
  • Austrian Science Fund FWF: P 21114
Ausgabe der Veröffentlichung
2
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Boraginaceae
  • Caspase 3
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation
  • Dose-Response Relationship, Drug
  • Drugs, Chinese Herbal
  • Ether
  • Humans
  • Neoplasms
  • Phytotherapy
  • Plant Roots
Sprache
eng
Country
Ireland
Paginierung
182 - 188
PII
S0378-8741(10)00121-2
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
129

Data source: PubMed

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