Berberine Inhibits Cell Growth and Mediates Caspase-Independent Cell Death in Human Pancreatic Cancer Cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Lina Pinto-Garcia
- Thomas Efferth
- Amada Torres
- Joerg D Hoheisel
- Mahmoud Youns
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000280370900013&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1055/s-0030-1249931
- eISSN
- 1439-0221
- Externe Identifier
- Clarivate Analytics Document Solution ID: 631TK
- PubMed Identifier: 20455200
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- PLANTA MEDICA
- Schlüsselwörter
- berberine
- pancreatic cancer
- microarray
- cell death
- photodynamic therapy
- Paginierung
- 1155 - 1161
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Berberine Inhibits Cell Growth and Mediates Caspase-Independent Cell Death in Human Pancreatic Cancer Cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 76
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Lina Pinto-Garcia
- Thomas Efferth
- Amada Torres
- Jörg Hoheisel
- Mahmoud Youns
- DOI
- 10.1055/s-0030-1249931
- eISSN
- 1439-0221
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- Planta Medica
- Sprache
- en
- Online publication date
- 2010
- Paginierung
- 1155 - 1161
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Herausgeber
- Georg Thieme Verlag KG
- Herausgeber URL
- http://dx.doi.org/10.1055/s-0030-1249931
- Datum der Datenerfassung
- 2017
- Titel
- Berberine Inhibits Cell Growth and Mediates Caspase-Independent Cell Death in Human Pancreatic Cancer Cells
- Ausgabe der Zeitschrift
- 76
Data source: Crossref
- Abstract
- Pancreatic cancer is one of the most aggressive human malignancies with an increasing incidence worldwide. In addition to the poor survival rates, combinations using gemcitabine as a backbone have failed to show any benefit beyond monotherapy. These facts underscore an urgent need for novel therapeutic options and motivated us to study the effect of berberine on pancreatic cancer cells. Here, we undertook an mRNA-based gene expression profiling study in order to get deeper insight into the molecular targets mediating the growth inhibitory effects of berberine on pancreatic cancer cells compared to normal ones. Twenty-four hours after treatment, berberine showed preferential selectivity toward pancreatic cancer cells compared to normal ones. Moreover, expression profiling and Ingenuity pathway analysis results showed that the cytotoxicity of berberine was accompanied with an activation of BRCA1-mediated DNA damage response, G1/S and G2/M cell cycle checkpoint regulation, and P53 signalling pathways. The activation of these signalling pathways might be explained by the fact that berberine intercalates DNA and induces DNA strand break through inhibition of topoisomerases and induction of DNA lesions.
- Addresses
- Department of Functional Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany.
- Autoren
- Lina Pinto-Garcia
- Thomas Efferth
- Thomas Efferth
- Amada Torres
- Jörg D Hoheisel
- Mahmoud Youns
- DOI
- 10.1055/s-0030-1249931
- eISSN
- 1439-0221
- Externe Identifier
- PubMed Identifier: 20455200
- Open access
- false
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- Planta medica
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Pancreatic Neoplasms
- Berberine
- Caspases
- RNA, Messenger
- Antineoplastic Agents, Phytogenic
- Oligonucleotide Array Sequence Analysis
- Immunohistochemistry
- Gene Expression Profiling
- Signal Transduction
- Apoptosis
- Cell Proliferation
- Enzyme Activation
- Caspase Inhibitors
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2010
- Paginierung
- 1155 - 1161
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum der Datenerfassung
- 2010
- Titel
- Berberine inhibits cell growth and mediates caspase-independent cell death in human pancreatic cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 76
Data source: Europe PubMed Central
- Abstract
- Pancreatic cancer is one of the most aggressive human malignancies with an increasing incidence worldwide. In addition to the poor survival rates, combinations using gemcitabine as a backbone have failed to show any benefit beyond monotherapy. These facts underscore an urgent need for novel therapeutic options and motivated us to study the effect of berberine on pancreatic cancer cells. Here, we undertook an mRNA-based gene expression profiling study in order to get deeper insight into the molecular targets mediating the growth inhibitory effects of berberine on pancreatic cancer cells compared to normal ones. Twenty-four hours after treatment, berberine showed preferential selectivity toward pancreatic cancer cells compared to normal ones. Moreover, expression profiling and Ingenuity pathway analysis results showed that the cytotoxicity of berberine was accompanied with an activation of BRCA1-mediated DNA damage response, G1/S and G2/M cell cycle checkpoint regulation, and P53 signalling pathways. The activation of these signalling pathways might be explained by the fact that berberine intercalates DNA and induces DNA strand break through inhibition of topoisomerases and induction of DNA lesions.
- Autoren
- Lina Pinto-Garcia
- Thomas Efferth
- Amada Torres
- Jörg D Hoheisel
- Mahmoud Youns
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20455200
- DOI
- 10.1055/s-0030-1249931
- eISSN
- 1439-0221
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- Planta Med
- Schlüsselwörter
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Berberine
- Caspase Inhibitors
- Caspases
- Cell Line, Tumor
- Cell Proliferation
- Enzyme Activation
- Gene Expression Profiling
- Humans
- Immunohistochemistry
- Oligonucleotide Array Sequence Analysis
- Pancreatic Neoplasms
- RNA, Messenger
- Signal Transduction
- Sprache
- eng
- Country
- Germany
- Paginierung
- 1155 - 1161
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Berberine inhibits cell growth and mediates caspase-independent cell death in human pancreatic cancer cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 76
Data source: PubMed
- Beziehungen:
- Property of