Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities

Publication type:
Journal article
Metadata:
Autoren
  • Nisarat Siriwatanametanon
  • Bernd L Fiebich
  • Thomas Efferth
  • Jose M Prieto
  • Michael Heinrich
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000280622400002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2010.04.036
Externe Identifier
  • Clarivate Analytics Document Solution ID: 634YO
  • PubMed Identifier: 20435130
ISSN
0378-8741
Ausgabe der Veröffentlichung
2
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • Anti-inflammatory
  • NF-kappa B
  • Pro-inflammatory cytokines (IL-6, IL-1 beta, TNF-alpha, PGE2)
  • Antioxidant
  • Total phenolic content
  • Anticancer
  • Thai medicinal plants
  • Gynura pseudochina var. hispida
  • Oroxylum indicum
  • Muehlenbeckia platyclada
Paginierung
196 - 207
Datum der Veröffentlichung
2010
Status
Published
Titel
Traditionally used Thai medicinal plants: <i>In vitro</i> anti-inflammatory, anticancer and antioxidant activities
Sub types
  • Article
Ausgabe der Zeitschrift
130

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Nisarat Siriwatanametanon
  • Bernd L Fiebich
  • Thomas Efferth
  • Jose M Prieto
  • Michael Heinrich
DOI
10.1016/j.jep.2010.04.036
ISSN
0378-8741
Ausgabe der Veröffentlichung
2
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
196 - 207
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2010.04.036
Datum der Datenerfassung
2021
Titel
Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities
Ausgabe der Zeitschrift
130

Data source: Crossref

Abstract
<h4>Aims of the study</h4>In order to assess traditional Thai claims about the therapeutic potential of medicinal plants and to select plants for future phytochemical research, nine plant species with anti-inflammatory uses were selected from Thai textbooks and assessed for their in vitro anti-inflammatory, antiproliferative and antioxidant activities.<h4>Methods</h4>Nuclear factor-kappaB (NF-kappaB) inhibitory effects in stably transfected HeLa cells were determined by luciferase assay, and effects on LPS-induced pro-inflammatory mediators prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1beta, and tumour necrosis factor (TNF)alpha in primary monocytes were assessed by ELISA. Cytotoxic activities were examined against HeLa cells, human leukaemia CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 subline using the MTT and XTT tests. However, a redox status has been linked with both inflammation and cancer, antioxidant effects were also assessed using the DPPH, lipid-peroxidation, and Folin-Ciocalteau methods.<h4>Results</h4>Among all the nine species, Gynura pseudochina var. hispida and Oroxylum indicum showed the most promising NF-kappaB inhibitory effects with the lowest IC(50) values (41.96 and 47.45 microg/ml, respectively). Muehlenbeckia platyclada did not inhibit the NF-kappaB activation but effectively inhibited the release of IL-6, IL-1beta and TNF-alpha with IC(50) values ranging between 0.28 and 8.67 microg/ml. Pouzolzia indica was the most cytotoxic against CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 cells (9.75% and 10.48% viability, at 10 microg/ml, respectively). Rhinacanthus nasutus was the most potent cytotoxicity against HeLa cells (IC(50) 3.63 microg/ml) and showed specific cytotoxicity against the multidrug-resistant CEM/ADR5000 cells (18.72% viability at 10 microg/ml, p<0.0001 when compared to its cytotoxicity against CCRF-CEM cells). Moreover, Oroxylum indicum showed a high level of antioxidant activity by inhibiting lipid-peroxidation (IC(50) 0.08 microg/ml).<h4>Conclusions</h4>This study provides in vitro evidence for the use of the Thai plants, most importantly Gynura pseudochina var. hispida, Oroxylum indicum and Muehlenbeckia platyclada as Thai anti-inflammatory remedies and these plants are now a priority for further phytochemical research.
Addresses
  • Centre for Pharmacognosy and Phytotherapy, The School of Pharmacy, University of London, London, United Kingdom.
Autoren
  • Nisarat Siriwatanametanon
  • Bernd L Fiebich
  • Thomas Efferth
  • Thomas Efferth
  • Jose M Prieto
  • Michael Heinrich
DOI
10.1016/j.jep.2010.04.036
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 20435130
Open access
false
ISSN
0378-8741
Ausgabe der Veröffentlichung
2
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Monocytes
  • Hela Cells
  • Humans
  • Asteraceae
  • Bignoniaceae
  • Polygonaceae
  • Plants, Medicinal
  • Phenols
  • Dinoprostone
  • Tumor Necrosis Factor-alpha
  • NF-kappa B
  • Anti-Inflammatory Agents
  • Inflammation Mediators
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Interleukin-6
  • Antioxidants
  • Medicine, Traditional
  • Transfection
  • Inhibitory Concentration 50
  • Cell Survival
  • Lipid Peroxidation
  • Dose-Response Relationship, Drug
  • Drug Resistance, Neoplasm
  • Thailand
  • Interleukin-1beta
  • Magnoliopsida
Sprache
eng
Medium
Print-Electronic
Online publication date
2010
Paginierung
196 - 207
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
130

Data source: Europe PubMed Central

Abstract
AIMS OF THE STUDY: In order to assess traditional Thai claims about the therapeutic potential of medicinal plants and to select plants for future phytochemical research, nine plant species with anti-inflammatory uses were selected from Thai textbooks and assessed for their in vitro anti-inflammatory, antiproliferative and antioxidant activities. METHODS: Nuclear factor-kappaB (NF-kappaB) inhibitory effects in stably transfected HeLa cells were determined by luciferase assay, and effects on LPS-induced pro-inflammatory mediators prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1beta, and tumour necrosis factor (TNF)alpha in primary monocytes were assessed by ELISA. Cytotoxic activities were examined against HeLa cells, human leukaemia CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 subline using the MTT and XTT tests. However, a redox status has been linked with both inflammation and cancer, antioxidant effects were also assessed using the DPPH, lipid-peroxidation, and Folin-Ciocalteau methods. RESULTS: Among all the nine species, Gynura pseudochina var. hispida and Oroxylum indicum showed the most promising NF-kappaB inhibitory effects with the lowest IC(50) values (41.96 and 47.45 microg/ml, respectively). Muehlenbeckia platyclada did not inhibit the NF-kappaB activation but effectively inhibited the release of IL-6, IL-1beta and TNF-alpha with IC(50) values ranging between 0.28 and 8.67 microg/ml. Pouzolzia indica was the most cytotoxic against CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 cells (9.75% and 10.48% viability, at 10 microg/ml, respectively). Rhinacanthus nasutus was the most potent cytotoxicity against HeLa cells (IC(50) 3.63 microg/ml) and showed specific cytotoxicity against the multidrug-resistant CEM/ADR5000 cells (18.72% viability at 10 microg/ml, p<0.0001 when compared to its cytotoxicity against CCRF-CEM cells). Moreover, Oroxylum indicum showed a high level of antioxidant activity by inhibiting lipid-peroxidation (IC(50) 0.08 microg/ml). CONCLUSIONS: This study provides in vitro evidence for the use of the Thai plants, most importantly Gynura pseudochina var. hispida, Oroxylum indicum and Muehlenbeckia platyclada as Thai anti-inflammatory remedies and these plants are now a priority for further phytochemical research.
Date of acceptance
2010
Autoren
  • Nisarat Siriwatanametanon
  • Bernd L Fiebich
  • Thomas Efferth
  • Jose M Prieto
  • Michael Heinrich
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20435130
DOI
10.1016/j.jep.2010.04.036
eISSN
1872-7573
Ausgabe der Veröffentlichung
2
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Anti-Inflammatory Agents
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Asteraceae
  • Bignoniaceae
  • Cell Survival
  • Dinoprostone
  • Dose-Response Relationship, Drug
  • Drug Resistance, Neoplasm
  • HeLa Cells
  • Humans
  • Inflammation Mediators
  • Inhibitory Concentration 50
  • Interleukin-1beta
  • Interleukin-6
  • Lipid Peroxidation
  • Magnoliopsida
  • Medicine, Traditional
  • Monocytes
  • NF-kappa B
  • Phenols
  • Plant Extracts
  • Plants, Medicinal
  • Polygonaceae
  • Thailand
  • Transfection
  • Tumor Necrosis Factor-alpha
Sprache
eng
Country
Ireland
Paginierung
196 - 207
PII
S0378-8741(10)00268-0
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
130

Data source: PubMed

Beziehungen:
Property of

Tools