Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots, induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial pathway
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Meng Luo
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Su Zhang
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000282391400019&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.cbi.2010.07.009
- eISSN
- 1872-7786
- Externe Identifier
- Clarivate Analytics Document Solution ID: 657DA
- PubMed Identifier: 20638373
- ISSN
- 0009-2797
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- CHEMICO-BIOLOGICAL INTERACTIONS
- Schlüsselwörter
- Cajanol
- Anticancer activity
- MCF-7
- Apoptosis
- Cell cycle arrest
- Paginierung
- 151 - 160
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Cajanol, a novel anticancer agent from Pigeonpea [<i>Cajanus</i> <i>cajan</i> (L.) Millsp.] roots, induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial pathway
- Sub types
- Article
- Ausgabe der Zeitschrift
- 188
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Meng Luo
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Su Zhang
- Liping Yao
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2010.07.009
- ISSN
- 0009-2797
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Chemico-Biological Interactions
- Sprache
- en
- Paginierung
- 151 - 160
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.cbi.2010.07.009
- Datum der Datenerfassung
- 2019
- Titel
- Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots, induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial pathway
- Ausgabe der Zeitschrift
- 188
Data source: Crossref
- Abstract
- Cajanol (5-hydroxy-3-(4-hydroxy-2-methoxyphenyl)-7-methoxychroman-4-one) is an isoflavanone from Pigeonpea [Cajanus cajan (L.) Millsp.] roots. As the most effective phytoalexin in pigeonpea, the cytotoxic activity of cajanol towards cancer cells has not been report as yet. In the present study, the anticancer activity of cajanol towards MCF-7 human breast cancer cells was investigated. In order to explore the underlying mechanism of cell growth inhibition of cajanol, cell cycle distribution, DNA fragmentation assay and morphological assessment of nuclear change, ROS generation, mitochondrial membrane potential (DeltaPsim) disruption, and expression of caspase-3 and caspase-9, Bax, Bcl-2, PARP and cytochrome c were measured in MCF-7 cells. Cajanol inhibited the growth of MCF-7 cells in a time and dose-dependent manner. The IC(50) value was 54.05 microM after 72 h treatment, 58.32 microM after 48 h; and 83.42 microM after 24h. Cajanol arrested the cell cycle in the G2/M phase and induced apoptosis via a ROS-mediated mitochondria-dependent pathway. Western blot analysis showed that cajanol inhibited Bcl-2 expression and induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release. Mitochondrial cytochrome c release was associated with the activation of caspase-9 and caspase-3 cascade, and active-caspase-3 was involved in PARP cleavage. All of these signal transduction pathways are involved in initiating apoptosis. To the best of our knowledge, this is the first report demonstrating the cytotoxic activity of cajanol towards cancer cells in vitro.
- Addresses
- Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
- Autoren
- Meng Luo
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Su Zhang
- Liping Yao
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2010.07.009
- eISSN
- 1872-7786
- Externe Identifier
- PubMed Identifier: 20638373
- Funding acknowledgements
- National Natural Science Foundation of China: 30770231
- Heilongjiang Province Science Foundation for Excellent Youths: JC200704
- Open access
- false
- ISSN
- 0009-2797
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Chemico-biological interactions
- Schlüsselwörter
- Cell Line, Tumor
- Mitochondria
- Humans
- Cajanus
- Plant Roots
- Breast Neoplasms
- Reactive Oxygen Species
- Isoflavones
- Antineoplastic Agents, Phytogenic
- Flow Cytometry
- Apoptosis
- Female
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2010
- Paginierung
- 151 - 160
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum der Datenerfassung
- 2010
- Titel
- Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots, induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial pathway.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 188
Data source: Europe PubMed Central
- Abstract
- Cajanol (5-hydroxy-3-(4-hydroxy-2-methoxyphenyl)-7-methoxychroman-4-one) is an isoflavanone from Pigeonpea [Cajanus cajan (L.) Millsp.] roots. As the most effective phytoalexin in pigeonpea, the cytotoxic activity of cajanol towards cancer cells has not been report as yet. In the present study, the anticancer activity of cajanol towards MCF-7 human breast cancer cells was investigated. In order to explore the underlying mechanism of cell growth inhibition of cajanol, cell cycle distribution, DNA fragmentation assay and morphological assessment of nuclear change, ROS generation, mitochondrial membrane potential (DeltaPsim) disruption, and expression of caspase-3 and caspase-9, Bax, Bcl-2, PARP and cytochrome c were measured in MCF-7 cells. Cajanol inhibited the growth of MCF-7 cells in a time and dose-dependent manner. The IC(50) value was 54.05 microM after 72 h treatment, 58.32 microM after 48 h; and 83.42 microM after 24h. Cajanol arrested the cell cycle in the G2/M phase and induced apoptosis via a ROS-mediated mitochondria-dependent pathway. Western blot analysis showed that cajanol inhibited Bcl-2 expression and induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release. Mitochondrial cytochrome c release was associated with the activation of caspase-9 and caspase-3 cascade, and active-caspase-3 was involved in PARP cleavage. All of these signal transduction pathways are involved in initiating apoptosis. To the best of our knowledge, this is the first report demonstrating the cytotoxic activity of cajanol towards cancer cells in vitro.
- Date of acceptance
- 2010
- Autoren
- Meng Luo
- Xia Liu
- Yuangang Zu
- Yujie Fu
- Su Zhang
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20638373
- DOI
- 10.1016/j.cbi.2010.07.009
- eISSN
- 1872-7786
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Chem Biol Interact
- Schlüsselwörter
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Breast Neoplasms
- Cajanus
- Cell Line, Tumor
- Female
- Flow Cytometry
- Humans
- Isoflavones
- Mitochondria
- Plant Roots
- Reactive Oxygen Species
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 151 - 160
- PII
- S0009-2797(10)00461-8
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots, induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial pathway.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 188
Data source: PubMed
- Beziehungen:
- Property of