Cajanol Inhibits the Growth of Escherichia coli and Staphylococcus aureus by Acting on Membrane and DNA Damage
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Xiao-lei Liu
- Xin-jian Zhang
- Yu-jie Fu
- Yuan-gang Zu
- Nan Wu
- Lu Liang
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000286461000010&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1055/s-0030-1250146
- eISSN
- 1439-0221
- Externe Identifier
- Clarivate Analytics Document Solution ID: 709SF
- PubMed Identifier: 20803417
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- PLANTA MEDICA
- Schlüsselwörter
- Cajanus cajan (L.) Millsp.
- pigeon pea
- cajanol
- lecithin
- phosphate groups
- DNA
- Paginierung
- 158 - 163
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Titel
- Cajanol Inhibits the Growth of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> by Acting on Membrane and DNA Damage
- Sub types
- Article
- Ausgabe der Zeitschrift
- 77
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Xiao-lei Liu
- Xin-jian Zhang
- Yu-jie Fu
- Yuan-gang Zu
- Nan Wu
- Lu Liang
- Thomas Efferth
- DOI
- 10.1055/s-0030-1250146
- eISSN
- 1439-0221
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 02
- Zeitschrift
- Planta Medica
- Sprache
- en
- Online publication date
- 2010
- Paginierung
- 158 - 163
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Herausgeber
- Georg Thieme Verlag KG
- Herausgeber URL
- http://dx.doi.org/10.1055/s-0030-1250146
- Datum der Datenerfassung
- 2017
- Titel
- Cajanol Inhibits the Growth of<i>Escherichia coli</i>and<i>Staphylococcus aureus</i>by Acting on Membrane and DNA Damage
- Ausgabe der Zeitschrift
- 77
Data source: Crossref
- Abstract
- In the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of Cajanus cajan (L.) Millsp. towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated. The antibacterial activity of cajanol was evaluated towards six bacterial strains (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa) by the broth microdilution method. It showed strong antibacterial activity towards all bacteria tested with minimal inhibition concentration (MIC) values ranging from 98.90 µM to 197.8 µM. Cajanol-induced death rates in the most sensitive strains ( E.COLI, 96.55 % and S. AUREUS, 97.25 %) were analyzed by flow cytometry. Furthermore, the activity of cajanol on the membranes of E. coli and S. aureus was investigated by using lecithin, phosphate groups, and fluorescence microscopy. Cajanol-induced DNA damage was observed by agarose gel electrophoresis. In summary, cajanol inhibited E. coli only by DNA damage, whereas S. aureus was inhibited by affecting both, the lecithin and phosphate groups on the cellular membrane and DNA. The present study shows that cajanol possesses antibacterial activity in vitro towards both gram-negative and gram-positive bacteria and therefore may be a promising candidate as an antibacterial agent for the therapy of microbial infections.
- Addresses
- Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.
- Autoren
- Xiao-lei Liu
- Xin-jian Zhang
- Yu-jie Fu
- Yuan-gang Zu
- Nan Wu
- Lu Liang
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1055/s-0030-1250146
- eISSN
- 1439-0221
- Externe Identifier
- PubMed Identifier: 20803417
- Open access
- false
- ISSN
- 0032-0943
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Planta medica
- Schlüsselwörter
- Cell Membrane
- Gram-Negative Bacteria
- Escherichia coli
- Gram-Positive Bacteria
- Staphylococcus aureus
- Cajanus
- Plant Roots
- DNA Damage
- Isoflavones
- Anti-Bacterial Agents
- Microbial Sensitivity Tests
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2010
- Paginierung
- 158 - 163
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum der Datenerfassung
- 2010
- Titel
- Cajanol inhibits the growth of Escherichia coli and Staphylococcus aureus by acting on membrane and DNA damage.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 77
Data source: Europe PubMed Central
- Abstract
- In the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of Cajanus cajan (L.) Millsp. towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated. The antibacterial activity of cajanol was evaluated towards six bacterial strains (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa) by the broth microdilution method. It showed strong antibacterial activity towards all bacteria tested with minimal inhibition concentration (MIC) values ranging from 98.90 µM to 197.8 µM. Cajanol-induced death rates in the most sensitive strains ( E.COLI, 96.55 % and S. AUREUS, 97.25 %) were analyzed by flow cytometry. Furthermore, the activity of cajanol on the membranes of E. coli and S. aureus was investigated by using lecithin, phosphate groups, and fluorescence microscopy. Cajanol-induced DNA damage was observed by agarose gel electrophoresis. In summary, cajanol inhibited E. coli only by DNA damage, whereas S. aureus was inhibited by affecting both, the lecithin and phosphate groups on the cellular membrane and DNA. The present study shows that cajanol possesses antibacterial activity in vitro towards both gram-negative and gram-positive bacteria and therefore may be a promising candidate as an antibacterial agent for the therapy of microbial infections.
- Autoren
- Xiao-lei Liu
- Xin-jian Zhang
- Yu-jie Fu
- Yuan-gang Zu
- Nan Wu
- Lu Liang
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20803417
- DOI
- 10.1055/s-0030-1250146
- eISSN
- 1439-0221
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Planta Med
- Schlüsselwörter
- Anti-Bacterial Agents
- Cajanus
- Cell Membrane
- DNA Damage
- Escherichia coli
- Gram-Negative Bacteria
- Gram-Positive Bacteria
- Isoflavones
- Microbial Sensitivity Tests
- Plant Roots
- Staphylococcus aureus
- Sprache
- eng
- Country
- Germany
- Paginierung
- 158 - 163
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2011
- Titel
- Cajanol inhibits the growth of Escherichia coli and Staphylococcus aureus by acting on membrane and DNA damage.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 77
Data source: PubMed
- Beziehungen:
- Property of