Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy

Publication type:
Journal article
Metadata:
Autoren
  • Nan Wu
  • Yu Kong
  • Yuangang Zu
  • Yujie Fu
  • Zhiguo Liu
  • Ronghua Meng
  • Xia Liu
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000287767800004&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2010.07.001
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: 726ZU
  • PubMed Identifier: 20739162
ISSN
0944-7113
Ausgabe der Veröffentlichung
2-3
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Pinostrobin
  • Cajanus cajan
  • HSV-1
  • MTT-assay
  • AFM
  • In vivo
Paginierung
110 - 118
Datum der Veröffentlichung
2011
Status
Published
Titel
Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy
Sub types
  • Article
Ausgabe der Zeitschrift
18

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Nan Wu
  • Yu Kong
  • Yuangang Zu
  • Yujie Fu
  • Zhiguo Liu
  • Ronghua Meng
  • Xia Liu
  • Thomas Efferth
DOI
10.1016/j.phymed.2010.07.001
ISSN
0944-7113
Ausgabe der Veröffentlichung
2-3
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
110 - 118
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2010.07.001
Datum der Datenerfassung
2022
Titel
Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy
Ausgabe der Zeitschrift
18

Data source: Crossref

Abstract
In the present study, the antiviral activity of pinostrobin towards herpes simplex virus-1 (HSV-1) was investigated by MTT assay and atomic force microscopy. Pinostrobin can inhibit HSV-1 replication with 50% effective concentration (EC(50)) of 22.71 ± 1.72 μg/ml. MTT assay showed HSV-1 was significantly inhibited when pretreated with pinostrobin, with the inhibition of 85.69 ± 2.59%. Significant changes in morphology and size of HSV-1 were observed by atomic force microscopy (AFM) in response to pinostrobin treatment. AFM topography and phase images showed that with increasing time, the envelope was shedded and damaged, finally leading to virus inactivation. With increasing concentration, pinostrobin caused a gradual leakage, also contributing to breakage of the envelope and virus inactivation. Treatment effect of oral pinostrobin in vivo showed that pinostrobin (50mg/kg/dose) possesses definite therapeutical effect in the development of lesion score. In general, the results showed that AFM represents a powerful technique for the investigation of morphology and size of HSV-1 treated by antiviral agents. AFM is applicable to study chemically induced morphological changes at the nanometer level.
Addresses
  • Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Autoren
  • Nan Wu
  • Yu Kong
  • Yuangang Zu
  • Yujie Fu
  • Zhiguo Liu
  • Ronghua Meng
  • Xia Liu
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.phymed.2010.07.001
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 20739162
Funding acknowledgements
  • Innovative Program for Importation of International Advanced Agricultural Science and Technology, National Forestry Bureau: 2006-4-75
  • National Key Technology R&D Program: 2006BAD18B0405
  • National Natural Science Foundation of China:
  • Key Project of Chinese Ministry of Education: 108049
  • Heilongjiang Province Science Foundation for Excellent Youths: 30770231
  • Heilongjiang Province Science Foundation for Excellent Youths: JC200704
  • Research Foundation for Science and Technology Innovation Talents of Harbin: 2006RFXXS001
Open access
false
ISSN
0944-7113
Ausgabe der Veröffentlichung
2-3
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Vero Cells
  • Animals
  • Mice, Inbred Strains
  • Mice
  • Herpesvirus 1, Human
  • Herpes Simplex
  • Flavanones
  • Plant Extracts
  • Microscopy, Atomic Force
  • Phytotherapy
  • Microbial Sensitivity Tests
  • Chlorocebus aethiops
Sprache
eng
Medium
Print-Electronic
Online publication date
2010
Paginierung
110 - 118
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2010
Titel
Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
18

Data source: Europe PubMed Central

Abstract
In the present study, the antiviral activity of pinostrobin towards herpes simplex virus-1 (HSV-1) was investigated by MTT assay and atomic force microscopy. Pinostrobin can inhibit HSV-1 replication with 50% effective concentration (EC(50)) of 22.71 ± 1.72 μg/ml. MTT assay showed HSV-1 was significantly inhibited when pretreated with pinostrobin, with the inhibition of 85.69 ± 2.59%. Significant changes in morphology and size of HSV-1 were observed by atomic force microscopy (AFM) in response to pinostrobin treatment. AFM topography and phase images showed that with increasing time, the envelope was shedded and damaged, finally leading to virus inactivation. With increasing concentration, pinostrobin caused a gradual leakage, also contributing to breakage of the envelope and virus inactivation. Treatment effect of oral pinostrobin in vivo showed that pinostrobin (50mg/kg/dose) possesses definite therapeutical effect in the development of lesion score. In general, the results showed that AFM represents a powerful technique for the investigation of morphology and size of HSV-1 treated by antiviral agents. AFM is applicable to study chemically induced morphological changes at the nanometer level.
Date of acceptance
2010
Autoren
  • Nan Wu
  • Yu Kong
  • Yuangang Zu
  • Yujie Fu
  • Zhiguo Liu
  • Ronghua Meng
  • Xia Liu
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20739162
DOI
10.1016/j.phymed.2010.07.001
eISSN
1618-095X
Ausgabe der Veröffentlichung
2-3
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Animals
  • Chlorocebus aethiops
  • Flavanones
  • Herpes Simplex
  • Herpesvirus 1, Human
  • Mice
  • Mice, Inbred Strains
  • Microbial Sensitivity Tests
  • Microscopy, Atomic Force
  • Phytotherapy
  • Plant Extracts
  • Vero Cells
Sprache
eng
Country
Germany
Paginierung
110 - 118
PII
S0944-7113(10)00223-0
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
18

Data source: PubMed

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