MicroRNA expression profile of MCF-7 human breast cancer cells and the effect of green tea polyphenon-60.

Publication type:
Journal article
Metadata:
Abstract
This study reports for the first time the microRNA expression profile of human breast cancer MCF-7 cells and the effect of green tea. Although hundreds of miRNAs have been identified in humans, only a small proportion (25.6%) of miRNAs are expressed in MCF-7 cells. Low concentration treatment with Polyphenon-60 significantly alters the miRNA expression profile in MCF-7 cells. Twenty three miRNAs have been identified with differential expression after a 48 h treatment with 10 μg/ml Polyphenon-60 (green tea extract). These miRNAs include miR-21 and miR-27 that were found to be down-regulated following treatment with green tea. These two miRNAs have previously been identified as being overexpressed in MCF-7 breast cancer cells, with miR-21 specifically implicated in down-regulating the tumor suppressor gene, tropomyosin-1. This data supports the hypothesis that Polyphenon-60-induced modification of the breast cancer miRNA expression profile contributes to the efficacy of green tea treatment. The resulting decrease in carcinogenesis is further supported by the altered miRNA regulation of potential oncogenes and tumor-suppressor genes.
Addresses
  • Department of Biology, East Carolina University, Greenville, NC 27858, U.S.A.
Autoren
  • Lindsey N Fix
  • Maitri Shah
  • Thomas Efferth
  • Mary A Farwell
  • Baohong Zhang
eISSN
1790-6245
Externe Identifier
  • PubMed Identifier: 20952761
Open access
false
ISSN
1109-6535
Ausgabe der Veröffentlichung
5
Zeitschrift
Cancer genomics & proteomics
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Camellia sinensis
  • Breast Neoplasms
  • Catechin
  • Tropomyosin
  • MicroRNAs
  • Plant Extracts
  • Antineoplastic Agents
  • Gene Expression Profiling
  • Polymerase Chain Reaction
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Oncogenes
  • Female
  • Biomarkers, Tumor
Sprache
eng
Medium
Print
Paginierung
261 - 277
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
MicroRNA expression profile of MCF-7 human breast cancer cells and the effect of green tea polyphenon-60.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
7

Data source: Europe PubMed Central

Other metadata sources:
Abstract
This study reports for the first time the microRNA expression profile of human breast cancer MCF-7 cells and the effect of green tea. Although hundreds of miRNAs have been identified in humans, only a small proportion (25.6%) of miRNAs are expressed in MCF-7 cells. Low concentration treatment with Polyphenon-60 significantly alters the miRNA expression profile in MCF-7 cells. Twenty three miRNAs have been identified with differential expression after a 48 h treatment with 10 μg/ml Polyphenon-60 (green tea extract). These miRNAs include miR-21 and miR-27 that were found to be down-regulated following treatment with green tea. These two miRNAs have previously been identified as being overexpressed in MCF-7 breast cancer cells, with miR-21 specifically implicated in down-regulating the tumor suppressor gene, tropomyosin-1. This data supports the hypothesis that Polyphenon-60-induced modification of the breast cancer miRNA expression profile contributes to the efficacy of green tea treatment. The resulting decrease in carcinogenesis is further supported by the altered miRNA regulation of potential oncogenes and tumor-suppressor genes.
Autoren
  • Lindsey N Fix
  • Maitri Shah
  • Thomas Efferth
  • Mary A Farwell
  • Baohong Zhang
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20952761
eISSN
1790-6245
Ausgabe der Veröffentlichung
5
Zeitschrift
Cancer Genomics Proteomics
Schlüsselwörter
  • Antineoplastic Agents
  • Biomarkers, Tumor
  • Breast Neoplasms
  • Camellia sinensis
  • Catechin
  • Cell Line, Tumor
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Humans
  • MicroRNAs
  • Oncogenes
  • Plant Extracts
  • Polymerase Chain Reaction
  • Tropomyosin
Sprache
eng
Country
Greece
Paginierung
261 - 277
PII
7/5/261
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
MicroRNA expression profile of MCF-7 human breast cancer cells and the effect of green tea polyphenon-60.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
7

Data source: PubMed

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