Establishment and comparative characterization of novel squamous cell non-small cell lung cancer cell lines and their corresponding tumor tissue

Publication type:
Journal article
Metadata:
Autoren
  • Sandra Gottschling
  • Anna Jauch
  • Ruprecht Kuner
  • Esther Herpel
  • Karin Mueller-Decker
  • Philipp A Schnabel
  • Elizabeth C Xu
  • Thomas Muley
  • Holger Sueltmann
  • Christian Bender
  • Martin Granzow
  • Thomas Efferth
  • Hans Hoffmann
  • Hendrik Dienemann
  • Felix JF Herth
  • Michael Meister
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000299450600007&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.lungcan.2011.05.020
Externe Identifier
  • Clarivate Analytics Document Solution ID: 881AB
  • PubMed Identifier: 21684623
ISSN
0169-5002
Ausgabe der Veröffentlichung
1
Zeitschrift
LUNG CANCER
Schlüsselwörter
  • Non-small cell lung cancer
  • Squamous cell carcinoma
  • Cell lines
  • Surface marker profile
  • Genotype
  • Gene expression profile
Paginierung
45 - 57
Datum der Veröffentlichung
2012
Status
Published
Titel
Establishment and comparative characterization of novel squamous cell non-small cell lung cancer cell lines and their corresponding tumor tissue
Sub types
  • Article
Ausgabe der Zeitschrift
75

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Sandra Gottschling
  • Anna Jauch
  • Ruprecht Kuner
  • Esther Herpel
  • Karin Mueller-Decker
  • Philipp A Schnabel
  • Elizabeth C Xu
  • Thomas Muley
  • Holger Sültmann
  • Christian Bender
  • Martin Granzow
  • Thomas Efferth
  • Hans Hoffmann
  • Hendrik Dienemann
  • Felix JF Herth
  • Michael Meister
DOI
10.1016/j.lungcan.2011.05.020
ISSN
0169-5002
Ausgabe der Veröffentlichung
1
Zeitschrift
Lung Cancer
Sprache
en
Paginierung
45 - 57
Datum der Veröffentlichung
2012
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.lungcan.2011.05.020
Datum der Datenerfassung
2022
Titel
Establishment and comparative characterization of novel squamous cell non-small cell lung cancer cell lines and their corresponding tumor tissue
Ausgabe der Zeitschrift
75

Data source: Crossref

Abstract
<h4>Background</h4>Cell lines play an important role for studying tumor biology and novel therapeutic agents. Particularly in pulmonary squamous cell carcinoma (SCC) the availability of cell lines is limited and knowledge about their representativeness for corresponding tumor tissue is scanty.<h4>Materials and methods</h4>We established three novel SCC cell lines from fresh tumor tissue of 28 donors, including 8 SCC. Two cell lines were derived from different localizations of the same donor, i.e. primary tumor and lymph node metastasis. This represents a so far unique combination in lung cancer. The genotypes, gene expression profiles and mutational status of epidermal growth factor receptor (EGF-R) and Kirsten rat sarcoma (k-ras) of the cell lines and their corresponding tumor tissue were analyzed and compared. Moreover, the molecular characteristics were related to functional properties of the cell lines. Those comprised proliferation, motility and chemosensitivity. The cell lines were authenticated by single tandem repeat DNA typing. Tumorigenicity was analyzed in a murine xenograft model.<h4>Results</h4>Comparative genomic hybridization and multiplex fluorescence in situ hybridization revealed essential genetic similarities between the cell lines and their corresponding tumor tissue, but indicated also some genetic evolution and clonal selection. EGF-R or k-ras mutations were not detected. Gene expression profiling showed various differences between tumor tissue and cell lines affecting gene clusters associated with immune response, adhesion, proliferation, differentiation and angiogenesis. However, there were also common gene expression patterns reflecting the relationship between cell lines and their corresponding tumor tissue. Moreover, the molecular characteristics of the tumor tissue and the descendent cell line were associated with functional properties of the latter. All cell lines showed a unique, heterozygous human DNA profile and one cell line displayed rapid tumor formation in mice.<h4>Conclusions</h4>Here, we demonstrate that cell lines represent a useful in vitro system for studying basic mechanisms in lung cancer, but cover only distinct molecular characteristics of the original tumor. Moreover, we present three novel, comprehensively characterized SCC cell lines.
Addresses
  • Dept. of Thoracic Oncology, Thoraxklinik/University of Heidelberg, Amalienstr. 5, 69126 Heidelberg, Germany. sandra.gottschling@thoraxklinik-heidelberg.de
Autoren
  • Sandra Gottschling
  • Anna Jauch
  • Ruprecht Kuner
  • Esther Herpel
  • Karin Mueller-Decker
  • Philipp A Schnabel
  • Elizabeth C Xu
  • Thomas Muley
  • Holger Sültmann
  • Christian Bender
  • Martin Granzow
  • Thomas Efferth
  • Thomas Efferth
  • Hans Hoffmann
  • Hendrik Dienemann
  • Felix JF Herth
  • Michael Meister
DOI
10.1016/j.lungcan.2011.05.020
eISSN
1872-8332
Externe Identifier
  • PubMed Identifier: 21684623
Open access
false
ISSN
0169-5002
Ausgabe der Veröffentlichung
1
Zeitschrift
Lung cancer (Amsterdam, Netherlands)
Schlüsselwörter
  • Cell Line, Tumor
  • Animals
  • Humans
  • Mice
  • Carcinoma, Non-Small-Cell Lung
  • Carcinoma, Squamous Cell
  • Lung Neoplasms
  • Lymphatic Metastasis
  • Neovascularization, Pathologic
  • In Situ Hybridization, Fluorescence
  • DNA Fingerprinting
  • Gene Expression Profiling
  • Carcinogenicity Tests
  • Cell Adhesion
  • Cell Differentiation
  • Cell Growth Processes
  • Cell Movement
  • Tandem Repeat Sequences
  • Cell Lineage
  • Mutation
  • Genes, ras
  • Comparative Genomic Hybridization
  • ErbB Receptors
Sprache
eng
Medium
Print
Paginierung
45 - 57
Datum der Veröffentlichung
2012
Status
Published
Datum der Datenerfassung
2011
Titel
Establishment and comparative characterization of novel squamous cell non-small cell lung cancer cell lines and their corresponding tumor tissue.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
75

Data source: Europe PubMed Central

Abstract
BACKGROUND: Cell lines play an important role for studying tumor biology and novel therapeutic agents. Particularly in pulmonary squamous cell carcinoma (SCC) the availability of cell lines is limited and knowledge about their representativeness for corresponding tumor tissue is scanty. MATERIALS AND METHODS: We established three novel SCC cell lines from fresh tumor tissue of 28 donors, including 8 SCC. Two cell lines were derived from different localizations of the same donor, i.e. primary tumor and lymph node metastasis. This represents a so far unique combination in lung cancer. The genotypes, gene expression profiles and mutational status of epidermal growth factor receptor (EGF-R) and Kirsten rat sarcoma (k-ras) of the cell lines and their corresponding tumor tissue were analyzed and compared. Moreover, the molecular characteristics were related to functional properties of the cell lines. Those comprised proliferation, motility and chemosensitivity. The cell lines were authenticated by single tandem repeat DNA typing. Tumorigenicity was analyzed in a murine xenograft model. RESULTS: Comparative genomic hybridization and multiplex fluorescence in situ hybridization revealed essential genetic similarities between the cell lines and their corresponding tumor tissue, but indicated also some genetic evolution and clonal selection. EGF-R or k-ras mutations were not detected. Gene expression profiling showed various differences between tumor tissue and cell lines affecting gene clusters associated with immune response, adhesion, proliferation, differentiation and angiogenesis. However, there were also common gene expression patterns reflecting the relationship between cell lines and their corresponding tumor tissue. Moreover, the molecular characteristics of the tumor tissue and the descendent cell line were associated with functional properties of the latter. All cell lines showed a unique, heterozygous human DNA profile and one cell line displayed rapid tumor formation in mice. CONCLUSIONS: Here, we demonstrate that cell lines represent a useful in vitro system for studying basic mechanisms in lung cancer, but cover only distinct molecular characteristics of the original tumor. Moreover, we present three novel, comprehensively characterized SCC cell lines.
Date of acceptance
2011
Autoren
  • Sandra Gottschling
  • Anna Jauch
  • Ruprecht Kuner
  • Esther Herpel
  • Karin Mueller-Decker
  • Philipp A Schnabel
  • Elizabeth C Xu
  • Thomas Muley
  • Holger Sültmann
  • Christian Bender
  • Martin Granzow
  • Thomas Efferth
  • Hans Hoffmann
  • Hendrik Dienemann
  • Felix JF Herth
  • Michael Meister
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21684623
DOI
10.1016/j.lungcan.2011.05.020
eISSN
1872-8332
Ausgabe der Veröffentlichung
1
Zeitschrift
Lung Cancer
Schlüsselwörter
  • Animals
  • Carcinogenicity Tests
  • Carcinoma, Non-Small-Cell Lung
  • Carcinoma, Squamous Cell
  • Cell Adhesion
  • Cell Differentiation
  • Cell Growth Processes
  • Cell Line, Tumor
  • Cell Lineage
  • Cell Movement
  • Comparative Genomic Hybridization
  • DNA Fingerprinting
  • ErbB Receptors
  • Gene Expression Profiling
  • Genes, ras
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lung Neoplasms
  • Lymphatic Metastasis
  • Mice
  • Mutation
  • Neovascularization, Pathologic
  • Tandem Repeat Sequences
Sprache
eng
Country
Ireland
Paginierung
45 - 57
PII
S0169-5002(11)00317-5
Datum der Veröffentlichung
2012
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2012
Titel
Establishment and comparative characterization of novel squamous cell non-small cell lung cancer cell lines and their corresponding tumor tissue.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
75

Data source: PubMed

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