Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Thomas Efferth
- Florian Herrmann
- Ahmed Tahrani
- Michael Wink
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000295748000008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2011.06.008
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 831RE
- PubMed Identifier: 21831619
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Artemisinin
- Cancer
- Drug resistance
- Microarray
- Natural products
- Pharmacogenomics
- Trypanosoma
- Paginierung
- 959 - 969
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Titel
- Cytotoxic activity of secondary metabolites derived from <i>Artemisia annua</i> L. towards cancer cells in comparison to its designated active constituent artemisinin
- Sub types
- Article
- Ausgabe der Zeitschrift
- 18
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Thomas Efferth
- Florian Herrmann
- Ahmed Tahrani
- Michael Wink
- DOI
- 10.1016/j.phymed.2011.06.008
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Paginierung
- 959 - 969
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2011.06.008
- Datum der Datenerfassung
- 2021
- Titel
- Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin
- Ausgabe der Zeitschrift
- 18
Data source: Crossref
- Abstract
- Artemisia annua L. (sweet wormwood, qinhao) has traditionally been used in Chinese medicine. The isolation of artemisinin from Artemisia annua and its worldwide accepted application in malaria therapy is one of the showcase success stories of phytomedicine during the past decades. Artemisinin-type compounds are also active towards other protozoal or viral diseases as well as cancer cells in vitro and in vivo. Nowadays, Artemisia annua tea is used as a self-reliant treatment in developing countries. The unsupervised use of Artemisia annua tea has been criticized to foster the development of artemisinin resistance in malaria and cancer due to insufficient artemisinin amounts in the plant as compared to standardized tablets with isolated artemisinin or semisynthetic artemisinin derivatives. However, artemisinin is not the only bioactive compound in Artemisia annua. In the present investigation, we analyzed different Artemisia annua extracts. Dichloromethane extracts were more cytotoxic (range of IC₅₀: 1.8-14.4 μg/ml) than methanol extracts towards Trypanosoma b. brucei (TC221 cells). The range of IC₅₀ values for HeLa cancer cells was 54.1-275.5 μg/ml for dichloromethane extracts and 276.3-1540.8 μg/ml for methanol extracts. Cancer and trypanosomal cells did not reveal cross-resistance among other compounds of Artemisia annua, namely the artemisinin-related artemisitene and arteanuine B as well as the unrelated compounds, scopoletin and 1,8-cineole. This indicates that cells resistant to one compound retained sensitivity to another one. These results were also supported by microarray-based mRNA expression profiling showing that molecular determinants of sensitivity and resistance were different between artemisinin and the other phytochemicals investigated.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germany. efferth@uni-mainz.de
- Autoren
- Thomas Efferth
- Thomas Efferth
- Florian Herrmann
- Ahmed Tahrani
- Michael Wink
- DOI
- 10.1016/j.phymed.2011.06.008
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 21831619
- Open access
- false
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Hela Cells
- Humans
- Trypanosoma brucei brucei
- Artemisia annua
- Cyclohexanols
- Monoterpenes
- Artemisinins
- Scopoletin
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Trypanocidal Agents
- Oligonucleotide Array Sequence Analysis
- Drug Screening Assays, Antitumor
- Parasitic Sensitivity Tests
- Molecular Structure
- Dose-Response Relationship, Drug
- Drug Resistance, Neoplasm
- Eucalyptol
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2011
- Paginierung
- 959 - 969
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum der Datenerfassung
- 2011
- Titel
- Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin.
- Sub types
- Comparative Study
- Journal Article
- Ausgabe der Zeitschrift
- 18
Data source: Europe PubMed Central
- Abstract
- Artemisia annua L. (sweet wormwood, qinhao) has traditionally been used in Chinese medicine. The isolation of artemisinin from Artemisia annua and its worldwide accepted application in malaria therapy is one of the showcase success stories of phytomedicine during the past decades. Artemisinin-type compounds are also active towards other protozoal or viral diseases as well as cancer cells in vitro and in vivo. Nowadays, Artemisia annua tea is used as a self-reliant treatment in developing countries. The unsupervised use of Artemisia annua tea has been criticized to foster the development of artemisinin resistance in malaria and cancer due to insufficient artemisinin amounts in the plant as compared to standardized tablets with isolated artemisinin or semisynthetic artemisinin derivatives. However, artemisinin is not the only bioactive compound in Artemisia annua. In the present investigation, we analyzed different Artemisia annua extracts. Dichloromethane extracts were more cytotoxic (range of IC₅₀: 1.8-14.4 μg/ml) than methanol extracts towards Trypanosoma b. brucei (TC221 cells). The range of IC₅₀ values for HeLa cancer cells was 54.1-275.5 μg/ml for dichloromethane extracts and 276.3-1540.8 μg/ml for methanol extracts. Cancer and trypanosomal cells did not reveal cross-resistance among other compounds of Artemisia annua, namely the artemisinin-related artemisitene and arteanuine B as well as the unrelated compounds, scopoletin and 1,8-cineole. This indicates that cells resistant to one compound retained sensitivity to another one. These results were also supported by microarray-based mRNA expression profiling showing that molecular determinants of sensitivity and resistance were different between artemisinin and the other phytochemicals investigated.
- Date of acceptance
- 2011
- Autoren
- Thomas Efferth
- Florian Herrmann
- Ahmed Tahrani
- Michael Wink
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/21831619
- DOI
- 10.1016/j.phymed.2011.06.008
- eISSN
- 1618-095X
- Ausgabe der Veröffentlichung
- 11
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Antineoplastic Agents, Phytogenic
- Artemisia annua
- Artemisinins
- Cyclohexanols
- Dose-Response Relationship, Drug
- Drug Resistance, Neoplasm
- Drug Screening Assays, Antitumor
- Eucalyptol
- HeLa Cells
- Humans
- Molecular Structure
- Monoterpenes
- Oligonucleotide Array Sequence Analysis
- Parasitic Sensitivity Tests
- Plant Extracts
- Scopoletin
- Trypanocidal Agents
- Trypanosoma brucei brucei
- Sprache
- eng
- Country
- Germany
- Paginierung
- 959 - 969
- PII
- S0944-7113(11)00193-0
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2011
- Titel
- Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin.
- Sub types
- Comparative Study
- Journal Article
- Ausgabe der Zeitschrift
- 18
Data source: PubMed
- Beziehungen:
- Property of