Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines

Publication type:
Journal article
Metadata:
Autoren
  • Xiao Shen
  • Tolga Eichhorn
  • Henry Johannes Greten
  • Thomas Efferth
DOI
10.3390/ph5020189
eISSN
1424-8247
Ausgabe der Veröffentlichung
2
Zeitschrift
Pharmaceuticals
Sprache
en
Online publication date
2012
Paginierung
189 - 208
Status
Published online
Herausgeber
MDPI AG
Herausgeber URL
http://dx.doi.org/10.3390/ph5020189
Datum der Datenerfassung
2018
Titel
Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
Ausgabe der Zeitschrift
5

Data source: Crossref

Other metadata sources:
Abstract
Scrophularia ningpoensis has been used in China for centuries as a herbal tea to treat various diseases. Based on the numerous animal studies on its pharmaceutical effects and the long time clinical experiences, we studied the molecular and cellular mechanism underlying the bioactivity of aqueous extract of Scrophularia and its isolated compounds. Seven isolated compounds, unlike Scrophularia extract, failed to induce cytotoxicity on HaCaT cells, but their combination improved the effect of extract. Tumor cell line selectivity was not observed, when we studied its cytotoxic effect on melanoma cell lines. The apoptotic and anti-inflammatory effects of Scrophularia extract have been demonstrated on HaCaT cells. The extract induced those effects potentially through affecting the MAPK pathway and inhibition of the NF-κB pathway, Microarray-based bioinformatical analyses on the compound acetoside from Scrophularia revealed a gene expression profile which confirmed our findings with the extract on proliferation inhibition, anti-inflammation and apoptosis. With DNA alkylation as major proposed mechanism of action, we assume acetoside as one of the active compounds in Scrophularia.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, Mainz 55128, Germany. efferth@uni-mainz.de.
Autoren
  • Xiao Shen
  • Tolga Eichhorn
  • Henry Johannes Greten
  • Thomas Efferth
  • Thomas Efferth
DOI
10.3390/ph5020189
eISSN
1424-8247
Externe Identifier
  • PubMed Identifier: 24288088
  • PubMed Central ID: PMC3763631
Open access
true
ISSN
1424-8247
Ausgabe der Veröffentlichung
2
Zeitschrift
Pharmaceuticals (Basel, Switzerland)
Sprache
eng
Medium
Electronic
Online publication date
2012
Open access status
Open Access
Paginierung
189 - 208
Datum der Veröffentlichung
2012
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2013
Titel
Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
5

Files

https://www.mdpi.com/1424-8247/5/2/189/pdf?version=1329406554 https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24288088/pdf/?tool=EBI https://europepmc.org/articles/PMC3763631?pdf=render

Data source: Europe PubMed Central

Abstract
Scrophularia ningpoensis has been used in China for centuries as a herbal tea to treat various diseases. Based on the numerous animal studies on its pharmaceutical effects and the long time clinical experiences, we studied the molecular and cellular mechanism underlying the bioactivity of aqueous extract of Scrophularia and its isolated compounds. Seven isolated compounds, unlike Scrophularia extract, failed to induce cytotoxicity on HaCaT cells, but their combination improved the effect of extract. Tumor cell line selectivity was not observed, when we studied its cytotoxic effect on melanoma cell lines. The apoptotic and anti-inflammatory effects of Scrophularia extract have been demonstrated on HaCaT cells. The extract induced those effects potentially through affecting the MAPK pathway and inhibition of the NF-κB pathway, Microarray-based bioinformatical analyses on the compound acetoside from Scrophularia revealed a gene expression profile which confirmed our findings with the extract on proliferation inhibition, anti-inflammation and apoptosis. With DNA alkylation as major proposed mechanism of action, we assume acetoside as one of the active compounds in Scrophularia.
Date of acceptance
2012
Autoren
  • Xiao Shen
  • Tolga Eichhorn
  • Henry Johannes Greten
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/24288088
DOI
10.3390/ph5020189
Externe Identifier
  • PubMed Central ID: PMC3763631
ISSN
1424-8247
Ausgabe der Veröffentlichung
2
Zeitschrift
Pharmaceuticals (Basel)
Sprache
eng
Country
Switzerland
Paginierung
189 - 208
PII
ph5020189
Datum der Veröffentlichung
2012
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2013
Titel
Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
5

Data source: PubMed

Beziehungen:
Property of

Tools