Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- T Eichhorn
- S Schloissnig
- B Hahn
- A Wendler
- Rolf Mertens
- WD Lehmann
- RL Krauth-Siegel
- T Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000301124600039&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1039/c2mb05437j
- eISSN
- 1742-2051
- Externe Identifier
- Clarivate Analytics Document Solution ID: 903NU
- PubMed Identifier: 22311186
- ISSN
- 1742-206X
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- MOLECULAR BIOSYSTEMS
- Paginierung
- 1311 - 1318
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Titel
- Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 8
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- T Eichhorn
- S Schloissnig
- B Hahn
- A Wendler
- Rolf Mertens
- WD Lehmann
- RL Krauth-Siegel
- T Efferth
- DOI
- 10.1039/c2mb05437j
- eISSN
- 1742-2051
- ISSN
- 1742-206X
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Molecular BioSystems
- Sprache
- en
- Paginierung
- 1311 - 1311
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Herausgeber
- Royal Society of Chemistry (RSC)
- Herausgeber URL
- http://dx.doi.org/10.1039/c2mb05437j
- Datum der Datenerfassung
- 2024
- Titel
- Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells
- Ausgabe der Zeitschrift
- 8
Data source: Crossref
- Abstract
- Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present investigation, we present a novel approach to identify artemisinin-interacting target proteins. Our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a surface without further chemical modification. The proteins identified effect among others, cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. Furthermore, a bioinformatic approach confirmed experimentally identified proteins and suggested a large number of other interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany.
- Autoren
- T Eichhorn
- S Schloissnig
- B Hahn
- A Wendler
- Rolf Mertens
- WD Lehmann
- RL Krauth-Siegel
- T Efferth
- T Efferth
- DOI
- 10.1039/c2mb05437j
- eISSN
- 1742-2051
- Externe Identifier
- PubMed Identifier: 22311186
- Open access
- false
- ISSN
- 1742-206X
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Molecular bioSystems
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Nasopharyngeal Neoplasms
- Artemisinins
- Neoplasm Proteins
- Anticarcinogenic Agents
- Proteomics
- Computational Biology
- Apoptosis
- Cell Proliferation
- Cell Cycle Checkpoints
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2012
- Paginierung
- 1311 - 1318
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum der Datenerfassung
- 2012
- Titel
- Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 8
Data source: Europe PubMed Central
- Abstract
- Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present investigation, we present a novel approach to identify artemisinin-interacting target proteins. Our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a surface without further chemical modification. The proteins identified effect among others, cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. Furthermore, a bioinformatic approach confirmed experimentally identified proteins and suggested a large number of other interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin.
- Autoren
- T Eichhorn
- S Schloissnig
- B Hahn
- A Wendler
- Rolf Mertens
- WD Lehmann
- RL Krauth-Siegel
- T Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/22311186
- DOI
- 10.1039/c2mb05437j
- eISSN
- 1742-2051
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Mol Biosyst
- Schlüsselwörter
- Anticarcinogenic Agents
- Apoptosis
- Artemisinins
- Cell Cycle Checkpoints
- Cell Line, Tumor
- Cell Proliferation
- Computational Biology
- Humans
- Nasopharyngeal Neoplasms
- Neoplasm Proteins
- Proteomics
- Sprache
- eng
- Country
- England
- Paginierung
- 1311 - 1318
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2012
- Titel
- Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 8
Data source: PubMed
- Beziehungen:
- Property of