A radiosensitizing effect of artesunate in glioblastoma cells is associated with a diminished expression of the inhibitor of apoptosis protein survivin
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Sebastian Reichert
- Vera Reinboldt
- Stephanie Hehlgans
- Thomas Efferth
- Claus Roedel
- Franz Roedel
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000305845000018&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.radonc.2012.03.018
- Externe Identifier
- Clarivate Analytics Document Solution ID: 966NP
- PubMed Identifier: 22560712
- ISSN
- 0167-8140
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- RADIOTHERAPY AND ONCOLOGY
- Schlüsselwörter
- Artesunate
- Glioblastoma
- Radiosensitization
- Survivin
- Paginierung
- 394 - 401
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Titel
- A radiosensitizing effect of artesunate in glioblastoma cells is associated with a diminished expression of the inhibitor of apoptosis protein survivin
- Sub types
- Article
- Ausgabe der Zeitschrift
- 103
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Sebastian Reichert
- Vera Reinboldt
- Stephanie Hehlgans
- Thomas Efferth
- Claus Rödel
- Franz Rödel
- DOI
- 10.1016/j.radonc.2012.03.018
- ISSN
- 0167-8140
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Radiotherapy and Oncology
- Sprache
- en
- Paginierung
- 394 - 401
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.radonc.2012.03.018
- Datum der Datenerfassung
- 2024
- Titel
- A radiosensitizing effect of artesunate in glioblastoma cells is associated with a diminished expression of the inhibitor of apoptosis protein survivin
- Ausgabe der Zeitschrift
- 103
Data source: Crossref
- Abstract
- <h4>Background and purpose</h4>Novel strategies to overcome an irradiation resistant phenotype may help to increase therapeutic efficacy in glioblastoma multiforme. The present study aimed to elucidate radiation sensitizing properties of artesunate, a semi synthetic derivate of artemisinin and to assess factors involved in this effect.<h4>Materials and methods</h4>LN229 and U87MG cells were treated with various concentrations of artesunate and radiation response was determined by a colony forming assay. Cell numbers, apoptosis induction, cell cycle distribution, and DNA repair following combined modality treatment were monitored by MTT-, caspase 3/7 assay, cytofluorometry, and γ-H2AX foci formation. Expression of survivin, survivin-GFP fusion protein, XIAP, cellular (c)IAP1 and cIAP2 was monitored by Western immunoblotting.<h4>Results</h4>Treatment of glioma cells with artesunate and irradiation resulted in an increased apoptotic fraction, pronounced G2/M arrest and increased DNA damage as demonstrated by an elevated amount of γ-H2AX foci/nucleus. Incubation with artesunate lowers survivin expression in a time and dose-dependent manner, whereas expression of XIAP, cIAP1 and cIAP2 was not affected. In clonogenic assays, treatment with artesunate revealed a significantly reduced surviving fraction, whereas stable over expression of a survivin-GFP protein reversed artesunate-mediated radiosensitization.<h4>Conclusion</h4>Artesunate selectively down regulates survivin that contributes to a radio-sensitization of glioma cells by an increased induction of apoptosis, cell cycle arrest, and a hampered DNA damage response.
- Addresses
- Department of Radiotherapy and Oncology, University of Frankfurt am Main, Germany.
- Autoren
- Sebastian Reichert
- Vera Reinboldt
- Stephanie Hehlgans
- Thomas Efferth
- Thomas Efferth
- Claus Rödel
- Franz Rödel
- DOI
- 10.1016/j.radonc.2012.03.018
- eISSN
- 1879-0887
- Externe Identifier
- PubMed Identifier: 22560712
- Open access
- false
- ISSN
- 0167-8140
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Glioblastoma
- Brain Neoplasms
- Artemisinins
- Neoplasm Proteins
- Radiation-Sensitizing Agents
- Cell Cycle
- Apoptosis
- Cell Survival
- DNA Repair
- Down-Regulation
- Dose-Response Relationship, Drug
- Inhibitor of Apoptosis Proteins
- Survivin
- Artesunate
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2012
- Paginierung
- 394 - 401
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum der Datenerfassung
- 2012
- Titel
- A radiosensitizing effect of artesunate in glioblastoma cells is associated with a diminished expression of the inhibitor of apoptosis protein survivin.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 103
Data source: Europe PubMed Central
- Abstract
- BACKGROUND AND PURPOSE: Novel strategies to overcome an irradiation resistant phenotype may help to increase therapeutic efficacy in glioblastoma multiforme. The present study aimed to elucidate radiation sensitizing properties of artesunate, a semi synthetic derivate of artemisinin and to assess factors involved in this effect. MATERIALS AND METHODS: LN229 and U87MG cells were treated with various concentrations of artesunate and radiation response was determined by a colony forming assay. Cell numbers, apoptosis induction, cell cycle distribution, and DNA repair following combined modality treatment were monitored by MTT-, caspase 3/7 assay, cytofluorometry, and γ-H2AX foci formation. Expression of survivin, survivin-GFP fusion protein, XIAP, cellular (c)IAP1 and cIAP2 was monitored by Western immunoblotting. RESULTS: Treatment of glioma cells with artesunate and irradiation resulted in an increased apoptotic fraction, pronounced G2/M arrest and increased DNA damage as demonstrated by an elevated amount of γ-H2AX foci/nucleus. Incubation with artesunate lowers survivin expression in a time and dose-dependent manner, whereas expression of XIAP, cIAP1 and cIAP2 was not affected. In clonogenic assays, treatment with artesunate revealed a significantly reduced surviving fraction, whereas stable over expression of a survivin-GFP protein reversed artesunate-mediated radiosensitization. CONCLUSION: Artesunate selectively down regulates survivin that contributes to a radio-sensitization of glioma cells by an increased induction of apoptosis, cell cycle arrest, and a hampered DNA damage response.
- Date of acceptance
- 2012
- Autoren
- Sebastian Reichert
- Vera Reinboldt
- Stephanie Hehlgans
- Thomas Efferth
- Claus Rödel
- Franz Rödel
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/22560712
- DOI
- 10.1016/j.radonc.2012.03.018
- eISSN
- 1879-0887
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Radiother Oncol
- Schlüsselwörter
- Apoptosis
- Artemisinins
- Artesunate
- Brain Neoplasms
- Cell Cycle
- Cell Line, Tumor
- Cell Survival
- DNA Repair
- Dose-Response Relationship, Drug
- Down-Regulation
- Glioblastoma
- Humans
- Inhibitor of Apoptosis Proteins
- Neoplasm Proteins
- Radiation-Sensitizing Agents
- Survivin
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 394 - 401
- PII
- S0167-8140(12)00152-1
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2012
- Titel
- A radiosensitizing effect of artesunate in glioblastoma cells is associated with a diminished expression of the inhibitor of apoptosis protein survivin.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 103
Data source: PubMed
- Beziehungen:
- Property of