Microarray-based mRNA Expression Profiling of Leukemia Cells Treated with the Flavonoid, Casticin

Publication type:
Journal article
Metadata:
Autoren
  • Chiara Righeschi
  • Tolga Eichhorn
  • Anastasia Karioti
  • Anna Rita Bilia
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000304429200005&DestLinkType=FullRecord&DestApp=WOS_CPL
eISSN
1790-6245
Externe Identifier
  • Clarivate Analytics Document Solution ID: 947KP
  • PubMed Identifier: 22593249
ISSN
1109-6535
Ausgabe der Veröffentlichung
3
Zeitschrift
CANCER GENOMICS & PROTEOMICS
Schlüsselwörter
  • Cancer
  • flavonoids
  • microarrays
  • multidrug resistance
  • natural products
  • pharmacogenomics
  • CCRF-CEM
  • CEM/ADR5000 cell lines
  • casticin
Paginierung
143 - 151
Datum der Veröffentlichung
2012
Status
Published
Titel
Microarray-based mRNA Expression Profiling of Leukemia Cells Treated with the Flavonoid, Casticin
Sub types
  • Article
Ausgabe der Zeitschrift
9

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Natural polyphenols play an important role in tumor inhibition. We used a doxorubicin-sensitive acute T-lymphoblastic leukemia cell line (CCRF-CEM) and its multidrug-resistant subline (CEM/ADR5000) to evaluate the activity of 15 plant polyphenols isolated in our laboratory (hypericin and pseudohypericin, verbascoside, ellagic acid, casticin, kaempferol-3-O-(2'',6''-di-E-p-coumaroyl)-glucopyranoside, kaempferol-3-O-(3,4-diacetyl-2,6-di-E-p-coumaroyl) -glucopyranoside, tiliroside, salvianolic acid B, oleuropein, rosmarinic acid, bergenin) or of others from commercial sources (curcumin, epigallocatechin-3-gallate, silymarin). Casticin was the most potent compound (IC50 values of 0.28 ± 0.02 μM in CCRF-CEM and 0.44 ± 0.17 μM in CEM/ADR5000 cells. The IC50 values of the other compounds tested ranged from 1.52 μM to 164.1 μM. A microarray-based mRNA expression profiling of CCRF-CEM cells treated with casticin was performed in order to identify genes with altered expression following casticin treatment. Networks related to NF-κB, p38MAPK, histones H3 and H4, and follicle stimulating hormone were identified.
Addresses
  • Department of Pharmaceutical Sciences, University of Florence, Florence, Italy.
Autoren
  • Chiara Righeschi
  • Tolga Eichhorn
  • Anastasia Karioti
  • Anna Rita Bilia
  • Thomas Efferth
eISSN
1790-6245
Externe Identifier
  • PubMed Identifier: 22593249
Open access
false
ISSN
1109-6535
Ausgabe der Veröffentlichung
3
Zeitschrift
Cancer genomics & proteomics
Schlüsselwörter
  • Cell Line, Tumor
  • Tumor Cells, Cultured
  • Humans
  • Leukemia
  • Flavonoids
  • RNA, Messenger
  • Microarray Analysis
  • Gene Expression Profiling
  • Female
  • Male
Sprache
eng
Medium
Print
Paginierung
143 - 151
Datum der Veröffentlichung
2012
Status
Published
Datum der Datenerfassung
2012
Titel
Microarray-based mRNA expression profiling of leukemia cells treated with the flavonoid, casticin.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Data source: Europe PubMed Central

Abstract
Natural polyphenols play an important role in tumor inhibition. We used a doxorubicin-sensitive acute T-lymphoblastic leukemia cell line (CCRF-CEM) and its multidrug-resistant subline (CEM/ADR5000) to evaluate the activity of 15 plant polyphenols isolated in our laboratory (hypericin and pseudohypericin, verbascoside, ellagic acid, casticin, kaempferol-3-O-(2'',6''-di-E-p-coumaroyl)-glucopyranoside, kaempferol-3-O-(3,4-diacetyl-2,6-di-E-p-coumaroyl) -glucopyranoside, tiliroside, salvianolic acid B, oleuropein, rosmarinic acid, bergenin) or of others from commercial sources (curcumin, epigallocatechin-3-gallate, silymarin). Casticin was the most potent compound (IC50 values of 0.28 ± 0.02 μM in CCRF-CEM and 0.44 ± 0.17 μM in CEM/ADR5000 cells. The IC50 values of the other compounds tested ranged from 1.52 μM to 164.1 μM. A microarray-based mRNA expression profiling of CCRF-CEM cells treated with casticin was performed in order to identify genes with altered expression following casticin treatment. Networks related to NF-κB, p38MAPK, histones H3 and H4, and follicle stimulating hormone were identified.
Autoren
  • Chiara Righeschi
  • Tolga Eichhorn
  • Anastasia Karioti
  • Anna Rita Bilia
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/22593249
eISSN
1790-6245
Ausgabe der Veröffentlichung
3
Zeitschrift
Cancer Genomics Proteomics
Schlüsselwörter
  • Cell Line, Tumor
  • Female
  • Flavonoids
  • Gene Expression Profiling
  • Humans
  • Leukemia
  • Male
  • Microarray Analysis
  • RNA, Messenger
  • Tumor Cells, Cultured
Sprache
eng
Country
Greece
Paginierung
143 - 151
PII
9/3/143
Datum der Veröffentlichung
2012
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2012
Titel
Microarray-based mRNA expression profiling of leukemia cells treated with the flavonoid, casticin.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Data source: PubMed

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