Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Ying Zhang
- Meng Luo
- Yuangang Zu
- Yujie Fu
- Chengbo Gu
- Wei Wang
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000309331700009&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.cbi.2012.06.007
- eISSN
- 1872-7786
- Externe Identifier
- Clarivate Analytics Document Solution ID: 013UP
- PubMed Identifier: 22796323
- ISSN
- 0009-2797
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- CHEMICO-BIOLOGICAL INTERACTIONS
- Schlüsselwörter
- Dryofragin
- Apoptosis
- MCF-7 cells
- Reactive oxygen species
- Paginierung
- 129 - 136
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Titel
- Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway
- Sub types
- Article
- Ausgabe der Zeitschrift
- 199
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Ying Zhang
- Meng Luo
- Yuangang Zu
- Yujie Fu
- Chengbo Gu
- Wei Wang
- Liping Yao
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2012.06.007
- ISSN
- 0009-2797
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Chemico-Biological Interactions
- Sprache
- en
- Paginierung
- 129 - 136
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.cbi.2012.06.007
- Datum der Datenerfassung
- 2022
- Titel
- Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway
- Ausgabe der Zeitschrift
- 199
Data source: Crossref
- Abstract
- Dryofragin is a phloroglucinol derivative extracted from Dryopteris fragrans (L.) Schott. In this study, the anticancer activity of dryofragin on human breast cancer MCF-7 cells was investigated. Dryofragin inhibited the growth of MCF-7 cells in a time and concentration-dependent manner. The cell viability was measured using MTT assay. After treatment with dryofragin for 72, 48 and 24 h, the IC₅₀ values were 27.26, 37.51 and 76.10 μM, respectively. Further analyses of DNA fragmentation and Annexin V-PI double-labeling indicated an induction of apoptosis. Dryofragin-treatment MCF-7 cells had a significantly accumulation of reactive oxygen species (ROS), as well as an increased percentage of cells with mitochondrial membrane potential (MMP) disruption. These phenomena were blocked by pretreatment for 2 h of MCF-7 cells with the antioxidant compound N-acetyl-L-cysteine (NAC, 5 mM). These results speak for the involvement of a ROS-mediated mitochondria-dependent pathway in dryofragin-induced apoptosis. Western blot results showed that dryofragin inhibited Bcl-2 and induced Bax expression which led to an activation of caspases-9 and -3 in the cytosol, and further cleavage of poly ADP-ribose polymerase (PARP) in the nucleus, then induced cell apoptosis. In conclusion, the present study provides evidence that dryofragin induces apoptosis in human breast cancer MCF-7 cells through a ROS-mediated mitochondrial pathway.
- Addresses
- Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
- Autoren
- Ying Zhang
- Meng Luo
- Yuangang Zu
- Yujie Fu
- Chengbo Gu
- Wei Wang
- Liping Yao
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.cbi.2012.06.007
- eISSN
- 1872-7786
- Externe Identifier
- PubMed Identifier: 22796323
- Funding acknowledgements
- Heilongjiang Province Science Foundation for Excellent Youths: JC200704
- Special Fund of Forestry Industrial Research for Public Welfare of China: 201004040
- Project for Distinguished Teacher Abroad, Chinese Ministry of Education: MS2010DBLY031
- Open access
- false
- ISSN
- 0009-2797
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Chemico-biological interactions
- Schlüsselwörter
- Breast
- Cell Line, Tumor
- Mitochondria
- Humans
- Dryopteris
- Breast Neoplasms
- Reactive Oxygen Species
- Phloroglucinol
- Antineoplastic Agents, Phytogenic
- Signal Transduction
- Apoptosis
- Female
- Membrane Potential, Mitochondrial
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2012
- Paginierung
- 129 - 136
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum der Datenerfassung
- 2012
- Titel
- Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 199
Data source: Europe PubMed Central
- Abstract
- Dryofragin is a phloroglucinol derivative extracted from Dryopteris fragrans (L.) Schott. In this study, the anticancer activity of dryofragin on human breast cancer MCF-7 cells was investigated. Dryofragin inhibited the growth of MCF-7 cells in a time and concentration-dependent manner. The cell viability was measured using MTT assay. After treatment with dryofragin for 72, 48 and 24 h, the IC₅₀ values were 27.26, 37.51 and 76.10 μM, respectively. Further analyses of DNA fragmentation and Annexin V-PI double-labeling indicated an induction of apoptosis. Dryofragin-treatment MCF-7 cells had a significantly accumulation of reactive oxygen species (ROS), as well as an increased percentage of cells with mitochondrial membrane potential (MMP) disruption. These phenomena were blocked by pretreatment for 2 h of MCF-7 cells with the antioxidant compound N-acetyl-L-cysteine (NAC, 5 mM). These results speak for the involvement of a ROS-mediated mitochondria-dependent pathway in dryofragin-induced apoptosis. Western blot results showed that dryofragin inhibited Bcl-2 and induced Bax expression which led to an activation of caspases-9 and -3 in the cytosol, and further cleavage of poly ADP-ribose polymerase (PARP) in the nucleus, then induced cell apoptosis. In conclusion, the present study provides evidence that dryofragin induces apoptosis in human breast cancer MCF-7 cells through a ROS-mediated mitochondrial pathway.
- Date of acceptance
- 2012
- Autoren
- Ying Zhang
- Meng Luo
- Yuangang Zu
- Yujie Fu
- Chengbo Gu
- Wei Wang
- Liping Yao
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/22796323
- DOI
- 10.1016/j.cbi.2012.06.007
- eISSN
- 1872-7786
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Chem Biol Interact
- Schlüsselwörter
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Breast
- Breast Neoplasms
- Cell Line, Tumor
- Dryopteris
- Female
- Humans
- Membrane Potential, Mitochondrial
- Mitochondria
- Phloroglucinol
- Reactive Oxygen Species
- Signal Transduction
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 129 - 136
- PII
- S0009-2797(12)00106-8
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2012
- Titel
- Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 199
Data source: PubMed
- Beziehungen:
- Property of