Growth-dependent release of carbohydrate metabolism-related and antioxidant enzymes from Staphylococcus aureus strain 6 as determined by proteomic analysis

Autoren

FV DONENKO
IM GRUBER
IB SEMENOVA
RG PRIYATKIN
RH ZIGANSHIN
EA ZARYADYEVA
OM IGNATOVA
EA KURBATOVA
MV KISELEVSKY
T EFFERTH

DOI

10.3892/etm.2011.346

eISSN

1792-1015

ISSN

1792-0981

Ausgabe der Veröffentlichung

6

Zeitschrift

Experimental and Therapeutic Medicine

Sprache

en

Online publication date

2011

Paginierung

1199 - 1204

Datum der Veröffentlichung

2011

Status

Published

Herausgeber

Spandidos Publications

Herausgeber URL

http://dx.doi.org/10.3892/etm.2011.346

Datum der Datenerfassung

2020

Titel

Growth-dependent release of carbohydrate metabolism-related and antioxidant enzymes from Staphylococcus aureus strain 6 as determined by proteomic analysis

Ausgabe der Zeitschrift

2

Data source: Crossref

Abstract

Proteins released into the culture medium by Staphylococcus aureus (S. aureus) strain 6 were determined at the end of the exponential growth phase (4.5 h). Eleven proteins were identified by liquid chromatography coupled with mass spectrometry. Three proteins were predicted to have signal peptides indicating their extracellular localization. The other proteins were presumably located in the cytoplasm of the bacteria. Five out of the 11 proteins were involved in carbohydrate metabolism. Other intracellular proteins of S. aureus were not detected in the culture medium. This indicates that the release of these 11 proteins was specific and that unspecific protein release due to damaged or dying bacteria did not play a role. It is suggested that enzymes associated with carbohydrate metabolism may provide the energy necessary for the transition of bacteria from a resting to a proliferative state. Another enzyme released by S. aureus, superoxide dismutase, may catalyze redox reactions in this context. The production of other proteolytic enzymes and toxins may take place at later stages of bacterial growth. A cocktail of these 11 proteins was used for the immunization of mice. Indeed, vaccination with these proteins prolonged the survival times of mice upon infection with S. aureus strain 6. Therefore, these proteins may have implications for the development of novel strategies for the prevention and therapy of S. aureus infections.

Addresses

N.N. Blokhin Russian Cancer Research Center and.

Autoren

FV Donenko
IM Gruber
IB Semenova
RG Priyatkin
RH Ziganshin
EA Zaryadyeva
OM Ignatova
EA Kurbatova
MV Kiselevsky
T Efferth
T Efferth

DOI

10.3892/etm.2011.346

eISSN

1792-1015

Externe Identifier

PubMed Identifier: 22977644
PubMed Central ID: PMC3440808

Open access

false

ISSN

1792-0981

Ausgabe der Veröffentlichung

6

Zeitschrift

Experimental and therapeutic medicine

Sprache

eng

Medium

Print-Electronic

Online publication date

2011

Paginierung

1199 - 1204

Datum der Veröffentlichung

2011

Status

Published

Datum der Datenerfassung

2012

Titel

Growth-dependent release of carbohydrate metabolism-related and antioxidant enzymes from Staphylococcus aureus strain 6 as determined by proteomic analysis.

Sub types

research-article
Journal Article

Ausgabe der Zeitschrift

2

Files

https://www.spandidos-publications.com/10.3892/etm.2011.346/download https://europepmc.org/articles/PMC3440808?pdf=render

Data source: Europe PubMed Central

Abstract

Proteins released into the culture medium by Staphylococcus aureus (S. aureus) strain 6 were determined at the end of the exponential growth phase (4.5 h). Eleven proteins were identified by liquid chromatography coupled with mass spectrometry. Three proteins were predicted to have signal peptides indicating their extracellular localization. The other proteins were presumably located in the cytoplasm of the bacteria. Five out of the 11 proteins were involved in carbohydrate metabolism. Other intracellular proteins of S. aureus were not detected in the culture medium. This indicates that the release of these 11 proteins was specific and that unspecific protein release due to damaged or dying bacteria did not play a role. It is suggested that enzymes associated with carbohydrate metabolism may provide the energy necessary for the transition of bacteria from a resting to a proliferative state. Another enzyme released by S. aureus, superoxide dismutase, may catalyze redox reactions in this context. The production of other proteolytic enzymes and toxins may take place at later stages of bacterial growth. A cocktail of these 11 proteins was used for the immunization of mice. Indeed, vaccination with these proteins prolonged the survival times of mice upon infection with S. aureus strain 6. Therefore, these proteins may have implications for the development of novel strategies for the prevention and therapy of S. aureus infections.

Date of acceptance

2011

Autoren

FV Donenko
IM Gruber
IB Semenova
RG Priyatkin
RH Ziganshin
EA Zaryadyeva
OM Ignatova
EA Kurbatova
MV Kiselevsky
T Efferth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/22977644

DOI

10.3892/etm.2011.346

Externe Identifier

PubMed Central ID: PMC3440808

ISSN

1792-0981

Ausgabe der Veröffentlichung

6

Zeitschrift

Exp Ther Med

Sprache

eng

Country

Greece

Paginierung

1199 - 1204

PII

etm-02-06-1199

Datum der Veröffentlichung

2011

Status

Published

Titel

Growth-dependent release of carbohydrate metabolism-related and antioxidant enzymes from Staphylococcus aureus strain 6 as determined by proteomic analysis.

Sub types

Journal Article

Ausgabe der Zeitschrift

2

Data source: PubMed

Growth-dependent release of carbohydrate metabolism-related and antioxidant enzymes from Staphylococcus aureus strain 6 as determined by proteomic analysis

Files

Tools