Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway

Publication type:
Journal article
Metadata:
Autoren
  • Yao Sun
  • Chang Gao
  • Meng Luo
  • Wei Wang
  • Chengbo Gu
  • Yuangang Zu
  • Ji Li
  • Thomas Efferth
  • Yujie Fu
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000315613300001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.cbi.2012.11.005
eISSN
1872-7786
Externe Identifier
  • Clarivate Analytics Document Solution ID: 099HY
  • PubMed Identifier: 23178381
ISSN
0009-2797
Ausgabe der Veröffentlichung
1-3
Zeitschrift
CHEMICO-BIOLOGICAL INTERACTIONS
Schlüsselwörter
  • Aspidin PB
  • Apoptosis
  • HepG2
  • PI3K/Akt/GSK3 beta
Paginierung
1 - 8
Datum der Veröffentlichung
2013
Status
Published
Titel
Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway
Sub types
  • Article
Ausgabe der Zeitschrift
201

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Yao Sun
  • Chang Gao
  • Meng Luo
  • Wei Wang
  • Chengbo Gu
  • Yuangang Zu
  • Ji Li
  • Thomas Efferth
  • Yujie Fu
DOI
10.1016/j.cbi.2012.11.005
ISSN
0009-2797
Ausgabe der Veröffentlichung
1-3
Zeitschrift
Chemico-Biological Interactions
Sprache
en
Paginierung
1 - 8
Datum der Veröffentlichung
2013
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.cbi.2012.11.005
Datum der Datenerfassung
2022
Titel
Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway
Ausgabe der Zeitschrift
201

Data source: Crossref

Abstract
Aspidin PB, a phloroglucinol derivative isolated from Dryopteris fragrans (L.) Schott, has been previously reported to exert high biological activities. In the present study, we analyzed the apoptotic mechanisms of aspidin PB on human hepatoma cell line, HepG2. Initially, aspidin PB was shown to inhibit the growth of HepG2 cells in a time and dose-dependent manner. After treatment with aspidin PB for 72 h, 48 h and 24 h using MTT assay, the IC(50) values were 10.59 μM, 20.86 μM and 46.59 μM, respectively. Aspidin PB was capable to induce apoptosis, as measured by mitochondrial membrane potential (ΔΨm), acridine orange (AO) staining and propidium iodide (PI)/annexin V-FITC double staining. To further explore the signaling pathway of aspidin PB-mediated apoptosis, we examined PI3K/Akt related proteins. Western blot analysis revealed that aspidin PB inhibited PI3K expression, phosphorylation of Ser473 Akt and Ser9 GSK3β followed by up-regulation of nonsteroidal anti-inflammatory drugs activated gene-1 (NAG-1) expression. Similarly, the effects of aspidin PB on PI3K, Akt, GSK3β, NAG-1 expression were abolished by treatment with the PI3K inhibitor, wortmannin. Taken together, our data suggested that the PI3K/Akt/GSK3β signal pathway may represent one of the major mechanisms of the effects of aspidin PB on human hepatocarcinoma cells.
Addresses
  • Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Autoren
  • Yao Sun
  • Chang Gao
  • Meng Luo
  • Wei Wang
  • Chengbo Gu
  • Yuangang Zu
  • Ji Li
  • Thomas Efferth
  • Thomas Efferth
  • Yujie Fu
DOI
10.1016/j.cbi.2012.11.005
eISSN
1872-7786
Externe Identifier
  • PubMed Identifier: 23178381
Open access
false
ISSN
0009-2797
Ausgabe der Veröffentlichung
1-3
Zeitschrift
Chemico-biological interactions
Schlüsselwörter
  • Humans
  • Carcinoma, Hepatocellular
  • Liver Neoplasms
  • Phloroglucinol
  • Androstadienes
  • Glycogen Synthase Kinase 3
  • Protein Kinase Inhibitors
  • Microscopy, Confocal
  • Flow Cytometry
  • Signal Transduction
  • Apoptosis
  • Cell Proliferation
  • Proto-Oncogene Proteins c-akt
  • Membrane Potential, Mitochondrial
  • Hep G2 Cells
  • Phosphatidylinositol 3-Kinases
  • Glycogen Synthase Kinase 3 beta
  • Wortmannin
  • Phosphoinositide-3 Kinase Inhibitors
Sprache
eng
Medium
Print-Electronic
Online publication date
2012
Paginierung
1 - 8
Datum der Veröffentlichung
2013
Status
Published
Datum der Datenerfassung
2012
Titel
Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
201

Data source: Europe PubMed Central

Abstract
Aspidin PB, a phloroglucinol derivative isolated from Dryopteris fragrans (L.) Schott, has been previously reported to exert high biological activities. In the present study, we analyzed the apoptotic mechanisms of aspidin PB on human hepatoma cell line, HepG2. Initially, aspidin PB was shown to inhibit the growth of HepG2 cells in a time and dose-dependent manner. After treatment with aspidin PB for 72 h, 48 h and 24 h using MTT assay, the IC(50) values were 10.59 μM, 20.86 μM and 46.59 μM, respectively. Aspidin PB was capable to induce apoptosis, as measured by mitochondrial membrane potential (ΔΨm), acridine orange (AO) staining and propidium iodide (PI)/annexin V-FITC double staining. To further explore the signaling pathway of aspidin PB-mediated apoptosis, we examined PI3K/Akt related proteins. Western blot analysis revealed that aspidin PB inhibited PI3K expression, phosphorylation of Ser473 Akt and Ser9 GSK3β followed by up-regulation of nonsteroidal anti-inflammatory drugs activated gene-1 (NAG-1) expression. Similarly, the effects of aspidin PB on PI3K, Akt, GSK3β, NAG-1 expression were abolished by treatment with the PI3K inhibitor, wortmannin. Taken together, our data suggested that the PI3K/Akt/GSK3β signal pathway may represent one of the major mechanisms of the effects of aspidin PB on human hepatocarcinoma cells.
Date of acceptance
2012
Autoren
  • Yao Sun
  • Chang Gao
  • Meng Luo
  • Wei Wang
  • Chengbo Gu
  • Yuangang Zu
  • Ji Li
  • Thomas Efferth
  • Yujie Fu
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/23178381
DOI
10.1016/j.cbi.2012.11.005
eISSN
1872-7786
Ausgabe der Veröffentlichung
1-3
Zeitschrift
Chem Biol Interact
Schlüsselwörter
  • Androstadienes
  • Apoptosis
  • Carcinoma, Hepatocellular
  • Cell Proliferation
  • Flow Cytometry
  • Glycogen Synthase Kinase 3
  • Glycogen Synthase Kinase 3 beta
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms
  • Membrane Potential, Mitochondrial
  • Microscopy, Confocal
  • Phloroglucinol
  • Phosphatidylinositol 3-Kinases
  • Phosphoinositide-3 Kinase Inhibitors
  • Protein Kinase Inhibitors
  • Proto-Oncogene Proteins c-akt
  • Signal Transduction
  • Wortmannin
Sprache
eng
Country
Ireland
Paginierung
1 - 8
PII
S0009-2797(12)00240-2
Datum der Veröffentlichung
2013
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2013
Titel
Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
201

Data source: PubMed

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