Dihydroquercetin (DHQ) Induced HO-1 and NQO1 Expression against Oxidative Stress through the Nrf2-Dependent Antioxidant Pathway

Autoren

Lu Liang
Chang Gao
Meng Luo
Wei Wang
Chunjian Zhao
Yuangang Zu
Thomas Efferth
Yujie Fu

DOI

10.1021/jf304768p

eISSN

1520-5118

ISSN

0021-8561

Ausgabe der Veröffentlichung

11

Zeitschrift

Journal of Agricultural and Food Chemistry

Sprache

en

Online publication date

2013

Paginierung

2755 - 2761

Datum der Veröffentlichung

2013

Status

Published

Herausgeber

American Chemical Society (ACS)

Herausgeber URL

http://dx.doi.org/10.1021/jf304768p

Datum der Datenerfassung

2023

Titel

Dihydroquercetin (DHQ) Induced HO-1 and NQO1 Expression against Oxidative Stress through the Nrf2-Dependent Antioxidant Pathway

Ausgabe der Zeitschrift

61

Data source: Crossref

Abstract

Dihydroquercetin (DHQ) is a well-known antioxidant agent. In the present investigation, we reported for the first time that DHQ stimulates the expression of phase II detoxifying enzymes through the Nrf2-dependent signaling pathway. The IC50 values of DHQ for reduction of 2,2-diphenyl-1-picrylhydrazol (DPPH), reducing power assay, lipid peroxidation assay, and xanthine oxidase inhibition were 5.96, 4.31, 2.03, and 13.24 μM, respectively. DHQ possessed considerable protective activity from oxidative DNA damage. A luciferase reporter assay also demonstrated that DHQ-activated signaling resulted in the increased transcriptional activity of Nrf2 through binding to the ARE (antioxidant response element) enhancer sequence. Furthermore, Western blotting and luciferase assay revealed DHQ activated ERK1/2, Akt, and JNK signaling pathways, subsequently leading to Nrf2 nuclear translocation. DHQ upregulated the Nrf2-related antioxidant genes heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase-1 (NQO1), and glutamate-cysteine ligase modifier subunits. Inhibition of Nrf2 by siRNA reduced DHQ-induced upregulation of these antioxidant genes.

Addresses

Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University , Harbin 150040, PR China.

Autoren

Lu Liang
Chang Gao
Meng Luo
Wei Wang
Chunjian Zhao
Yuangang Zu
Thomas Efferth
Thomas Efferth
Yujie Fu

DOI

10.1021/jf304768p

eISSN

1520-5118

Externe Identifier

PubMed Identifier: 23419114

Open access

false

ISSN

0021-8561

Ausgabe der Veröffentlichung

11

Zeitschrift

Journal of agricultural and food chemistry

Schlüsselwörter

Humans
Larix
Quercetin
NAD(P)H Dehydrogenase (Quinone)
Plant Extracts
Antioxidants
Signal Transduction
Up-Regulation
Oxidative Stress
NF-E2-Related Factor 2
Heme Oxygenase-1
Transcriptional Activation
Hep G2 Cells
Antioxidant Response Elements

Sprache

eng

Medium

Print-Electronic

Online publication date

2013

Paginierung

2755 - 2761

Datum der Veröffentlichung

2013

Status

Published

Datum der Datenerfassung

2013

Titel

Dihydroquercetin (DHQ) induced HO-1 and NQO1 expression against oxidative stress through the Nrf2-dependent antioxidant pathway.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

61

Data source: Europe PubMed Central

Abstract

Dihydroquercetin (DHQ) is a well-known antioxidant agent. In the present investigation, we reported for the first time that DHQ stimulates the expression of phase II detoxifying enzymes through the Nrf2-dependent signaling pathway. The IC50 values of DHQ for reduction of 2,2-diphenyl-1-picrylhydrazol (DPPH), reducing power assay, lipid peroxidation assay, and xanthine oxidase inhibition were 5.96, 4.31, 2.03, and 13.24 μM, respectively. DHQ possessed considerable protective activity from oxidative DNA damage. A luciferase reporter assay also demonstrated that DHQ-activated signaling resulted in the increased transcriptional activity of Nrf2 through binding to the ARE (antioxidant response element) enhancer sequence. Furthermore, Western blotting and luciferase assay revealed DHQ activated ERK1/2, Akt, and JNK signaling pathways, subsequently leading to Nrf2 nuclear translocation. DHQ upregulated the Nrf2-related antioxidant genes heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase-1 (NQO1), and glutamate-cysteine ligase modifier subunits. Inhibition of Nrf2 by siRNA reduced DHQ-induced upregulation of these antioxidant genes.

Autoren

Lu Liang
Chang Gao
Meng Luo
Wei Wang
Chunjian Zhao
Yuangang Zu
Thomas Efferth
Yujie Fu

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/23419114

DOI

10.1021/jf304768p

eISSN

1520-5118

Ausgabe der Veröffentlichung

11

Zeitschrift

J Agric Food Chem

Schlüsselwörter

Antioxidant Response Elements
Antioxidants
Heme Oxygenase-1
Hep G2 Cells
Humans
Larix
NAD(P)H Dehydrogenase (Quinone)
NF-E2-Related Factor 2
Oxidative Stress
Plant Extracts
Quercetin
Signal Transduction
Transcriptional Activation
Up-Regulation

Sprache

eng

Country

United States

Paginierung

2755 - 2761

Datum der Veröffentlichung

2013

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2014

Titel

Dihydroquercetin (DHQ) induced HO-1 and NQO1 expression against oxidative stress through the Nrf2-dependent antioxidant pathway.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

61

Data source: PubMed

Dihydroquercetin (DHQ) Induced HO-1 and NQO1 Expression against Oxidative Stress through the Nrf2-Dependent Antioxidant Pathway

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