Cytotoxicity and Pharmacogenomics of Medicinal Plants from Traditional Korean Medicine

Publication type:
Journal article
Metadata:
Autoren
  • Victor Kuete
  • Ean-Jeong Seo
  • Benjamin Krusche
  • Mira Oswald
  • Benjamin Wiench
  • Sven Schroeder
  • Henry Johannes Greten
  • Ik-Soo Lee
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000322229600001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1155/2013/341724
eISSN
1741-4288
Externe Identifier
  • Clarivate Analytics Document Solution ID: 188WY
  • PubMed Identifier: 23935662
ISSN
1741-427X
Zeitschrift
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
Artikelnummer
ARTN 341724
Datum der Veröffentlichung
2013
Status
Published
Titel
Cytotoxicity and Pharmacogenomics of Medicinal Plants from Traditional Korean Medicine
Sub types
  • Article
Ausgabe der Zeitschrift
2013

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:p><jats:italic>Aim</jats:italic>. The present study was designed to investigate the cytotoxicity of a panel of 280 Korean medicinal plants belonging to 73 families and 198 species against human CCRF-CEM leukemia cells. Selected phytochemicals were investigated in more detail for their mode of action.<jats:italic>Methods</jats:italic>. The resazurin assay was used to determine cytotoxicity of the plant extracts. Microarray-based mRNA expression profiling, COMPARE, and hierarchical cluster analyses were applied to identify which genes correlate with sensitivity or resistance to selected phytochemicals of the Korean plants.<jats:italic>Results</jats:italic>. The results of the resazurin assay showed that cytotoxicity extracts tested at 10 <jats:italic>μ</jats:italic>g/mL from 13 samples inhibited proliferation more than 50% (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mtext>I</mml:mtext><mml:msub><mml:mrow><mml:mtext>C</mml:mtext></mml:mrow><mml:mrow><mml:mn>50</mml:mn></mml:mrow></mml:msub><mml:mo>&lt;</mml:mo><mml:mn>10</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL) and the most active plants are<jats:italic>Sedum middendorffianum</jats:italic>(15.33%)<jats:italic>and Lycoris radiata</jats:italic>(17.61%). Out of 13 selected phytochemicals from these plants, hopeaphenol and deoxynarciclasine were the most cytotoxic ones. Genes from various functional groups (transcriptional or translational regulation, signal transduction, cellular proliferation, intracellular trafficking, RNA metabolism, endoplasmic/sarcoplasmic reticulum function, etc.) were significantly correlated with response of tumor cell lines to these two compounds.<jats:italic>Conclusion</jats:italic>. The results provide evidence on the possible use of selected Korean medicinal plants and chemical constituents derived from them for the treatment of tumors.</jats:p>
Autoren
  • Victor Kuete
  • Ean-Jeong Seo
  • Benjamin Krusche
  • Mira Oswald
  • Benjamin Wiench
  • Sven Schröder
  • Henry Johannes Greten
  • Ik-Soo Lee
  • Thomas Efferth
DOI
10.1155/2013/341724
eISSN
1741-4288
ISSN
1741-427X
Zeitschrift
Evidence-Based Complementary and Alternative Medicine
Sprache
en
Paginierung
1 - 14
Datum der Veröffentlichung
2013
Status
Published
Herausgeber
Hindawi Limited
Herausgeber URL
http://dx.doi.org/10.1155/2013/341724
Datum der Datenerfassung
2019
Titel
Cytotoxicity and Pharmacogenomics of Medicinal Plants from Traditional Korean Medicine
Ausgabe der Zeitschrift
2013

Data source: Crossref

Abstract
Aim. The present study was designed to investigate the cytotoxicity of a panel of 280 Korean medicinal plants belonging to 73 families and 198 species against human CCRF-CEM leukemia cells. Selected phytochemicals were investigated in more detail for their mode of action. Methods. The resazurin assay was used to determine cytotoxicity of the plant extracts. Microarray-based mRNA expression profiling, COMPARE, and hierarchical cluster analyses were applied to identify which genes correlate with sensitivity or resistance to selected phytochemicals of the Korean plants. Results. The results of the resazurin assay showed that cytotoxicity extracts tested at 10  μ g/mL from 13 samples inhibited proliferation more than 50% (IC50 < 10  μ g/mL) and the most active plants are Sedum middendorffianum (15.33%) and Lycoris radiata (17.61%). Out of 13 selected phytochemicals from these plants, hopeaphenol and deoxynarciclasine were the most cytotoxic ones. Genes from various functional groups (transcriptional or translational regulation, signal transduction, cellular proliferation, intracellular trafficking, RNA metabolism, endoplasmic/sarcoplasmic reticulum function, etc.) were significantly correlated with response of tumor cell lines to these two compounds. Conclusion. The results provide evidence on the possible use of selected Korean medicinal plants and chemical constituents derived from them for the treatment of tumors.
Addresses
  • Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon ; Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
Autoren
  • Victor Kuete
  • Ean-Jeong Seo
  • Benjamin Krusche
  • Mira Oswald
  • Benjamin Wiench
  • Sven Schröder
  • Henry Johannes Greten
  • Ik-Soo Lee
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1155/2013/341724
eISSN
1741-4288
Externe Identifier
  • PubMed Identifier: 23935662
  • PubMed Central ID: PMC3725712
Open access
true
ISSN
1741-427X
Zeitschrift
Evidence-based complementary and alternative medicine : eCAM
Sprache
eng
Medium
Print-Electronic
Online publication date
2013
Open access status
Open Access
Paginierung
341724
Datum der Veröffentlichung
2013
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2013
Titel
Cytotoxicity and pharmacogenomics of medicinal plants from traditional korean medicine.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
2013

Files

https://downloads.hindawi.com/journals/ecam/2013/341724.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935662/pdf/?tool=EBI https://europepmc.org/articles/PMC3725712?pdf=render

Data source: Europe PubMed Central

Abstract
Aim. The present study was designed to investigate the cytotoxicity of a panel of 280 Korean medicinal plants belonging to 73 families and 198 species against human CCRF-CEM leukemia cells. Selected phytochemicals were investigated in more detail for their mode of action. Methods. The resazurin assay was used to determine cytotoxicity of the plant extracts. Microarray-based mRNA expression profiling, COMPARE, and hierarchical cluster analyses were applied to identify which genes correlate with sensitivity or resistance to selected phytochemicals of the Korean plants. Results. The results of the resazurin assay showed that cytotoxicity extracts tested at 10  μ g/mL from 13 samples inhibited proliferation more than 50% (IC50 < 10  μ g/mL) and the most active plants are Sedum middendorffianum (15.33%) and Lycoris radiata (17.61%). Out of 13 selected phytochemicals from these plants, hopeaphenol and deoxynarciclasine were the most cytotoxic ones. Genes from various functional groups (transcriptional or translational regulation, signal transduction, cellular proliferation, intracellular trafficking, RNA metabolism, endoplasmic/sarcoplasmic reticulum function, etc.) were significantly correlated with response of tumor cell lines to these two compounds. Conclusion. The results provide evidence on the possible use of selected Korean medicinal plants and chemical constituents derived from them for the treatment of tumors.
Date of acceptance
2013
Autoren
  • Victor Kuete
  • Ean-Jeong Seo
  • Benjamin Krusche
  • Mira Oswald
  • Benjamin Wiench
  • Sven Schröder
  • Henry Johannes Greten
  • Ik-Soo Lee
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/23935662
DOI
10.1155/2013/341724
Externe Identifier
  • PubMed Central ID: PMC3725712
ISSN
1741-427X
Zeitschrift
Evid Based Complement Alternat Med
Sprache
eng
Country
United States
Paginierung
341724
Datum der Veröffentlichung
2013
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2013
Titel
Cytotoxicity and pharmacogenomics of medicinal plants from traditional korean medicine.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
2013

Data source: PubMed

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