Cytotoxicity and modes of action of three naturally occurring xanthones (8-hydroxycudraxanthone G, morusignin I and cudraxanthone I) against sensitive and multidrug-resistant cancer cell lines
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Victor Kuete
- Louis P Sandjo
- Judith L Nantchouang Ouete
- Hugues Fouotsa
- Benjamin Wiench
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000334012400018&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.phymed.2013.08.018
- eISSN
- 1618-095X
- Externe Identifier
- Clarivate Analytics Document Solution ID: AE5FE
- PubMed Identifier: 24075210
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- PHYTOMEDICINE
- Schlüsselwörter
- Xanthones
- Natural products
- Cudraxanthone I
- Cytotoxicity
- Cell cycle distribution
- Mitochondrial membrane potential
- Apoptosis
- Paginierung
- 315 - 322
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- Cytotoxicity and modes of action of three naturally occurring xanthones (8-hydroxycudraxanthone G, morusignin I and cudraxanthone I) against sensitive and multidrug-resistant cancer cell lines
- Sub types
- Article
- Ausgabe der Zeitschrift
- 21
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Victor Kuete
- Louis P Sandjo
- Judith L Nantchouang Ouete
- Hugues Fouotsa
- Benjamin Wiench
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2013.08.018
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Phytomedicine
- Sprache
- en
- Paginierung
- 315 - 322
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.phymed.2013.08.018
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity and modes of action of three naturally occurring xanthones (8-hydroxycudraxanthone G, morusignin I and cudraxanthone I) against sensitive and multidrug-resistant cancer cell lines
- Ausgabe der Zeitschrift
- 21
Data source: Crossref
- Abstract
- <h4>Background</h4>Resistance of cancer to chemotherapy remains a challenging issue for scientists as well as physicians. Naturally occurring xanthones possess a variety of biological activities such as anti-inflammatory, anti-bacterial, and anti-cancer effects. The present study was aimed at investigating the cytotoxicity and the modes of action of three naturally occurring xanthones namely, morusignin I (1), 8-hydroxycudraxanthone G (2) and cudraxanthone I (3) against a panel of nine cancer cell lines, including various sensitive and drug-resistant phenotypes.<h4>Methods</h4>The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9 in cells treated with compounds 3. Flow cytometry was used for cell cycle analysis and detection of apoptotic cells, analysis of mitochondrial membrane potential (MMP) as well as measurement of reactive oxygen species (ROS).<h4>Results</h4>Compounds 1 and 3 inhibited the proliferation of all tested cancer cell lines including sensitive and drug-resistant phenotypes. Compound 2 was active on 8/9 cell lines with the IC50 values ranging from 16.65 μM (against leukemia CCRF-CEM cells) to 70.38 μM (against hepatocarcinoma HepG2 cells). The IC50 value ranged from 7.15 μM (against CCRF-CEM cells) to 53.85 μM [against human glioblastoma U87MG.ΔEGFR cells] for compound 1, and from 2.78 μM (against breast cancer MDA-MB231 BCRP cells) to 22.49 μM (against U87MG cells) for compound 3. P-glycoprotein expressing CEM/ADR5000 cells were cross-resistant to compounds 1 and 2 (4.21- to 610-fold) while no cross-resistance or even collateral cross-sensitivity were observed in other drug-resistant cell lines to the three compounds. Normal AML12 liver cells were more resistant to the three compounds than HepG2 liver cancer cells. Compounds 3 arrested the cell cycle between G0/G1 and S phases, strongly induced apoptosis via caspases 3/7, caspase 8, caspase 9 activation and disrupted the MMP in CCRF-CEM cells.<h4>Conclusions</h4>The cytotoxicity of the studied xanthones and especially compound 3 deserve more detailed exploration in the future to develop novel anticancer drugs against sensitive and otherwise drug-resistant phenotypes.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon.
- Autoren
- Victor Kuete
- Louis P Sandjo
- Judith L Nantchouang Ouete
- Hugues Fouotsa
- Benjamin Wiench
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.phymed.2013.08.018
- eISSN
- 1618-095X
- Externe Identifier
- PubMed Identifier: 24075210
- Open access
- false
- ISSN
- 0944-7113
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Garcinia
- Neoplasms
- Xanthones
- Caspases
- Plant Extracts
- Antineoplastic Agents, Phytogenic
- Phytotherapy
- Drug Resistance, Multiple
- Apoptosis
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Hep G2 Cells
- Cell Cycle Checkpoints
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2013
- Paginierung
- 315 - 322
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum der Datenerfassung
- 2013
- Titel
- Cytotoxicity and modes of action of three naturally occurring xanthones (8-hydroxycudraxanthone G, morusignin I and cudraxanthone I) against sensitive and multidrug-resistant cancer cell lines.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 21
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Resistance of cancer to chemotherapy remains a challenging issue for scientists as well as physicians. Naturally occurring xanthones possess a variety of biological activities such as anti-inflammatory, anti-bacterial, and anti-cancer effects. The present study was aimed at investigating the cytotoxicity and the modes of action of three naturally occurring xanthones namely, morusignin I (1), 8-hydroxycudraxanthone G (2) and cudraxanthone I (3) against a panel of nine cancer cell lines, including various sensitive and drug-resistant phenotypes. METHODS: The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9 in cells treated with compounds 3. Flow cytometry was used for cell cycle analysis and detection of apoptotic cells, analysis of mitochondrial membrane potential (MMP) as well as measurement of reactive oxygen species (ROS). RESULTS: Compounds 1 and 3 inhibited the proliferation of all tested cancer cell lines including sensitive and drug-resistant phenotypes. Compound 2 was active on 8/9 cell lines with the IC50 values ranging from 16.65 μM (against leukemia CCRF-CEM cells) to 70.38 μM (against hepatocarcinoma HepG2 cells). The IC50 value ranged from 7.15 μM (against CCRF-CEM cells) to 53.85 μM [against human glioblastoma U87MG.ΔEGFR cells] for compound 1, and from 2.78 μM (against breast cancer MDA-MB231 BCRP cells) to 22.49 μM (against U87MG cells) for compound 3. P-glycoprotein expressing CEM/ADR5000 cells were cross-resistant to compounds 1 and 2 (4.21- to 610-fold) while no cross-resistance or even collateral cross-sensitivity were observed in other drug-resistant cell lines to the three compounds. Normal AML12 liver cells were more resistant to the three compounds than HepG2 liver cancer cells. Compounds 3 arrested the cell cycle between G0/G1 and S phases, strongly induced apoptosis via caspases 3/7, caspase 8, caspase 9 activation and disrupted the MMP in CCRF-CEM cells. CONCLUSIONS: The cytotoxicity of the studied xanthones and especially compound 3 deserve more detailed exploration in the future to develop novel anticancer drugs against sensitive and otherwise drug-resistant phenotypes.
- Date of acceptance
- 2013
- Autoren
- Victor Kuete
- Louis P Sandjo
- Judith L Nantchouang Ouete
- Hugues Fouotsa
- Benjamin Wiench
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24075210
- DOI
- 10.1016/j.phymed.2013.08.018
- eISSN
- 1618-095X
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Phytomedicine
- Schlüsselwörter
- Apoptosis
- Cell cycle distribution
- Cudraxanthone I
- Cytotoxicity
- Mitochondrial membrane potential
- Natural products
- Xanthones
- Antineoplastic Agents, Phytogenic
- Apoptosis
- Caspases
- Cell Cycle Checkpoints
- Cell Line, Tumor
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Garcinia
- Hep G2 Cells
- Humans
- Membrane Potential, Mitochondrial
- Neoplasms
- Phytotherapy
- Plant Extracts
- Xanthones
- Sprache
- eng
- Country
- Germany
- Paginierung
- 315 - 322
- PII
- S0944-7113(13)00321-8
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- Cytotoxicity and modes of action of three naturally occurring xanthones (8-hydroxycudraxanthone G, morusignin I and cudraxanthone I) against sensitive and multidrug-resistant cancer cell lines.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 21
Data source: PubMed
- Beziehungen:
- Property of