A phenolic ester from Aglaia loheri leaves reveals cytotoxicity towards sensitive and multidrug-resistant cancer cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Else Dapat
- Sonia Jacinto
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000328369200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/1472-6882-13-286
- eISSN
- 1472-6882
- Externe Identifier
- Clarivate Analytics Document Solution ID: 271CX
- Zeitschrift
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Schlüsselwörter
- Aglaia loheri
- Cytotoxicity
- Multi-drug resistance
- Apoptosis
- JC-1 mitochondrial membrane potential
- Annexin V-FITC
- Artikelnummer
- ARTN 286
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Titel
- A phenolic ester from <i>Aglaia loheri</i> leaves reveals cytotoxicity towards sensitive and multidrug-resistant cancer cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 13
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Else Dapat
- Sonia Jacinto
- Thomas Efferth
- DOI
- 10.1186/1472-6882-13-286
- eISSN
- 1472-6882
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complementary and Alternative Medicine
- Sprache
- en
- Artikelnummer
- 286
- Online publication date
- 2013
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/1472-6882-13-286
- Datum der Datenerfassung
- 2022
- Titel
- A phenolic ester from Aglaia loheri leaves reveals cytotoxicity towards sensitive and multidrug-resistant cancer cells
- Ausgabe der Zeitschrift
- 13
Data source: Crossref
- Abstract
- <h4>Background</h4>Bioactivity-guided fractionation of extracts of Aglaia loheri Blanco (Meliaceae) yielded a cytotoxic isolate, termed Maldi 531.2[M + H]+. This phenolic ester was further investigated for its in vitro cytotoxicity toward human CCRF-CEM leukemia cells and their multi-drug resistant (MDR) subline, CEM/ADR5000. The intrinsic mitochondrial membrane potential (ΔΨm) and induction of apoptosis by this isolate were evaluated.<h4>Methods</h4>Chromatography techniques, mass spectrometry and proton NMR were employed to isolate Maldi 531.2[M + H]+. XTT cell proliferation and viability assay was used for cytotoxic test, and JC-1[5',5',6,6',-tetrachloro-1,1',3,3'-tetraethylbenzimidazoyl carbocyanine iodide was used to assess ΔΨm and initiation of apoptosis; Annexin V/FITC-PI staining was employed to analyse apoptosis.<h4>Results</h4>Maldi 531.2[M + H]+ was cytotoxic towards both CCRF-CEM and CEM/ADR5000 cells with IC50 values of 0.02 and 0.03 μM, respectively. The mitochondrial membrane potential (ΔΨm) of MDR cells was significantly reduced in a dose-dependent manner leading to apoptosis as detected by flow cytometric Annexin V-FITC/ PI staining.<h4>Conclusion</h4>Maldi 531.2[M + H]+ may be a potential anti-cancer drug candidate whose mode of action include reduction of the mitochondrial membrane potential and induction of apoptosis.
- Addresses
- Department of Biology, University of the Philippines, Manila City, Philippines. elsegdapat40@gmail.com.
- Autoren
- Else Dapat
- Sonia Jacinto
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1186/1472-6882-13-286
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Identifier: 24160768
- PubMed Central ID: PMC3816152
- Open access
- true
- ISSN
- 1472-6882
- Zeitschrift
- BMC complementary and alternative medicine
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Aglaia
- Leukemia
- Ascorbic Acid
- Phenols
- Plant Extracts
- Antineoplastic Agents
- Apoptosis
- Cell Proliferation
- Drug Resistance, Neoplasm
- Membrane Potential, Mitochondrial
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2013
- Open access status
- Open Access
- Paginierung
- 286
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2013
- Titel
- A phenolic ester from Aglaia loheri leaves reveals cytotoxicity towards sensitive and multidrug-resistant cancer cells.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 13
Files
https://bmccomplementmedtherapies.biomedcentral.com/counter/pdf/10.1186/1472-6882-13-286 https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24160768/pdf/?tool=EBI https://europepmc.org/articles/PMC3816152?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Bioactivity-guided fractionation of extracts of Aglaia loheri Blanco (Meliaceae) yielded a cytotoxic isolate, termed Maldi 531.2[M + H]+. This phenolic ester was further investigated for its in vitro cytotoxicity toward human CCRF-CEM leukemia cells and their multi-drug resistant (MDR) subline, CEM/ADR5000. The intrinsic mitochondrial membrane potential (ΔΨm) and induction of apoptosis by this isolate were evaluated. METHODS: Chromatography techniques, mass spectrometry and proton NMR were employed to isolate Maldi 531.2[M + H]+. XTT cell proliferation and viability assay was used for cytotoxic test, and JC-1[5',5',6,6',-tetrachloro-1,1',3,3'-tetraethylbenzimidazoyl carbocyanine iodide was used to assess ΔΨm and initiation of apoptosis; Annexin V/FITC-PI staining was employed to analyse apoptosis. RESULTS: Maldi 531.2[M + H]+ was cytotoxic towards both CCRF-CEM and CEM/ADR5000 cells with IC50 values of 0.02 and 0.03 μM, respectively. The mitochondrial membrane potential (ΔΨm) of MDR cells was significantly reduced in a dose-dependent manner leading to apoptosis as detected by flow cytometric Annexin V-FITC/ PI staining. CONCLUSION: Maldi 531.2[M + H]+ may be a potential anti-cancer drug candidate whose mode of action include reduction of the mitochondrial membrane potential and induction of apoptosis.
- Date of acceptance
- 2013
- Autoren
- Else Dapat
- Sonia Jacinto
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24160768
- DOI
- 10.1186/1472-6882-13-286
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Central ID: PMC3816152
- Zeitschrift
- BMC Complement Altern Med
- Schlüsselwörter
- Aglaia
- Antineoplastic Agents
- Apoptosis
- Ascorbic Acid
- Cell Line, Tumor
- Cell Proliferation
- Drug Resistance, Neoplasm
- Humans
- Leukemia
- Membrane Potential, Mitochondrial
- Phenols
- Plant Extracts
- Sprache
- eng
- Country
- England
- Paginierung
- 286
- PII
- 1472-6882-13-286
- Datum der Veröffentlichung
- 2013
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- A phenolic ester from Aglaia loheri leaves reveals cytotoxicity towards sensitive and multidrug-resistant cancer cells.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 13
Data source: PubMed
- Beziehungen:
- Property of