Activity of three cytotoxic isoflavonoids from Erythrina excelsa and Erythrina senegalensis (neobavaisoflavone, sigmoidin H and isoneorautenol) toward multi-factorial drug resistant cancer cells

Publication type:
Journal article
Metadata:
Autoren
  • Victor Kuete
  • Louis P Sandjo
  • Guy MN Kwamou
  • Benjamin Wiench
  • Augustin E Nkengfack
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000334726800015&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2013.10.017
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: AF5AQ
  • PubMed Identifier: 24252341
ISSN
0944-7113
Ausgabe der Veröffentlichung
5
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Isoflavonoids
  • Pterocarpan
  • Cytotoxicity
  • Cell cycle distribution
  • Mitochondrial membrane potential
  • Apoptosis
Paginierung
682 - 688
Datum der Veröffentlichung
2014
Status
Published
Titel
Activity of three cytotoxic isoflavonoids from <i>Erythrina excelsa</i> and <i>Erythrina senegalensis</i> (neobavaisoflavone, sigmoidin H and isoneorautenol) toward multi-factorial drug resistant cancer cells
Sub types
  • Article
Ausgabe der Zeitschrift
21

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Victor Kuete
  • Louis P Sandjo
  • Guy MN Kwamou
  • Benjamin Wiench
  • Augustin E Nkengfack
  • Thomas Efferth
DOI
10.1016/j.phymed.2013.10.017
ISSN
0944-7113
Ausgabe der Veröffentlichung
5
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
682 - 688
Datum der Veröffentlichung
2014
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2013.10.017
Datum der Datenerfassung
2023
Titel
Activity of three cytotoxic isoflavonoids from Erythrina excelsa and Erythrina senegalensis (neobavaisoflavone, sigmoidin H and isoneorautenol) toward multi-factorial drug resistant cancer cells
Ausgabe der Zeitschrift
21

Data source: Crossref

Abstract
<h4>Introduction</h4>Resistance of cancer cells to chemotherapy has become a worldwide concern. Naturally occuring isoflavonoids possess a variety of biological activities including anti-cancer effects. The present study was aimed at investigating the cytotoxicity and the modes of action of three naturally occuring isoflavonoids, neobavaisoflavone (1), sigmoidin H (2) and a pterocarpan that is a special type of isoflavonoid, isoneorautenol (3) against a panel of nine cancer cell lines, including various sensitive and drug-resistant phenotypes.<h4>Methods</h4>The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9 in cells treated with compounds 3. Flow cytometry was used for cell cycle analysis and detection of apoptotic cells, analysis of mitochondrial membrane potential (MMP) as well as measurement of reactive oxygen species (ROS).<h4>Results</h4>Compounds 3 showed significant cytotoxicity toward sensitive and drug-resistant cancer cell lines. Compounds 1 and 2 were selectively active, and IC50 values below 115 μM were obtained on 6/9 and 4/9 cell lines respectively with values ranging from 42.93 μM (toward CCRF-CEM cells) to 114.64 μM [against HCT116 (p53(+/+)) cells] for 1 and 25.59 μM (toward U87MG) to 110.51 μM [against HCT116 (p53(+/+)) cells] for 2. IC50 values ranging from 2.67 μM (against MDA-MB 237BCRP cells) to 21.84 (toward U87MG) were measured for compound 3 and between 0.20 μM (toward CCRF-CEM cells) and 195.12 μM (toward CEM/ADR5000 cells) for doxorubicin as control drug. BCRP-transfected MDA-MB-231 cells, HCT116 (p53(+/+)) and U87MG.ΔEGFR cells were hypersensitive (collateral sensitive) to compound 3 as compared to their counterpart cell lines. Compound 3 induced apoptosis in CCRF-CEM cells via activation of caspases 3/7, 8 and 9 as well as the loss of MMP and increased ROS production.<h4>Conclusions</h4>The cytotoxicity of the studied isoflavonoids and especially the pterocarpan 3 deserve more detailed exploration in the future to develop novel anticancer drugs against sensitive and otherwise drug-resistant phenotypes.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon.
Autoren
  • Victor Kuete
  • Louis P Sandjo
  • Guy MN Kwamou
  • Benjamin Wiench
  • Augustin E Nkengfack
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.phymed.2013.10.017
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 24252341
Open access
false
ISSN
0944-7113
Ausgabe der Veröffentlichung
5
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • HCT116 Cells
  • Humans
  • Erythrina
  • Reactive Oxygen Species
  • Benzofurans
  • Benzopyrans
  • Isoflavones
  • Caspases
  • Antineoplastic Agents, Phytogenic
  • Drug Screening Assays, Antitumor
  • Cell Cycle
  • Apoptosis
  • Drug Resistance, Neoplasm
  • Membrane Potential, Mitochondrial
Sprache
eng
Medium
Print-Electronic
Online publication date
2013
Paginierung
682 - 688
Datum der Veröffentlichung
2014
Status
Published
Datum der Datenerfassung
2013
Titel
Activity of three cytotoxic isoflavonoids from Erythrina excelsa and Erythrina senegalensis (neobavaisoflavone, sigmoidin H and isoneorautenol) toward multi-factorial drug resistant cancer cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
21

Data source: Europe PubMed Central

Abstract
INTRODUCTION: Resistance of cancer cells to chemotherapy has become a worldwide concern. Naturally occuring isoflavonoids possess a variety of biological activities including anti-cancer effects. The present study was aimed at investigating the cytotoxicity and the modes of action of three naturally occuring isoflavonoids, neobavaisoflavone (1), sigmoidin H (2) and a pterocarpan that is a special type of isoflavonoid, isoneorautenol (3) against a panel of nine cancer cell lines, including various sensitive and drug-resistant phenotypes. METHODS: The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9 in cells treated with compounds 3. Flow cytometry was used for cell cycle analysis and detection of apoptotic cells, analysis of mitochondrial membrane potential (MMP) as well as measurement of reactive oxygen species (ROS). RESULTS: Compounds 3 showed significant cytotoxicity toward sensitive and drug-resistant cancer cell lines. Compounds 1 and 2 were selectively active, and IC50 values below 115 μM were obtained on 6/9 and 4/9 cell lines respectively with values ranging from 42.93 μM (toward CCRF-CEM cells) to 114.64 μM [against HCT116 (p53(+/+)) cells] for 1 and 25.59 μM (toward U87MG) to 110.51 μM [against HCT116 (p53(+/+)) cells] for 2. IC50 values ranging from 2.67 μM (against MDA-MB 237BCRP cells) to 21.84 (toward U87MG) were measured for compound 3 and between 0.20 μM (toward CCRF-CEM cells) and 195.12 μM (toward CEM/ADR5000 cells) for doxorubicin as control drug. BCRP-transfected MDA-MB-231 cells, HCT116 (p53(+/+)) and U87MG.ΔEGFR cells were hypersensitive (collateral sensitive) to compound 3 as compared to their counterpart cell lines. Compound 3 induced apoptosis in CCRF-CEM cells via activation of caspases 3/7, 8 and 9 as well as the loss of MMP and increased ROS production. CONCLUSIONS: The cytotoxicity of the studied isoflavonoids and especially the pterocarpan 3 deserve more detailed exploration in the future to develop novel anticancer drugs against sensitive and otherwise drug-resistant phenotypes.
Date of acceptance
2013
Autoren
  • Victor Kuete
  • Louis P Sandjo
  • Guy MN Kwamou
  • Benjamin Wiench
  • Augustin E Nkengfack
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/24252341
DOI
10.1016/j.phymed.2013.10.017
eISSN
1618-095X
Ausgabe der Veröffentlichung
5
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Apoptosis
  • Cell cycle distribution
  • Cytotoxicity
  • Isoflavonoids
  • Mitochondrial membrane potential
  • Pterocarpan
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Benzofurans
  • Benzopyrans
  • Caspases
  • Cell Cycle
  • Drug Resistance, Neoplasm
  • Drug Screening Assays, Antitumor
  • Erythrina
  • HCT116 Cells
  • Humans
  • Isoflavones
  • Membrane Potential, Mitochondrial
  • Reactive Oxygen Species
Sprache
eng
Country
Germany
Paginierung
682 - 688
PII
S0944-7113(13)00426-1
Datum der Veröffentlichung
2014
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2014
Titel
Activity of three cytotoxic isoflavonoids from Erythrina excelsa and Erythrina senegalensis (neobavaisoflavone, sigmoidin H and isoneorautenol) toward multi-factorial drug resistant cancer cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
21

Data source: PubMed

Beziehungen:
Property of

Tools