Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells

Publication type:
Journal article
Metadata:
Autoren
  • Thomas Friedemann
  • Benjamin Otto
  • Kristin Klaetschke
  • Udo Schumacher
  • Yi Tao
  • Alexander Kai-Man Leung
  • Thomas Efferth
  • Sven Schroeder
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000340854000060&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jep.2014.06.004
eISSN
1872-7573
Externe Identifier
  • Clarivate Analytics Document Solution ID: AN8KV
  • PubMed Identifier: 24929105
ISSN
0378-8741
Ausgabe der Veröffentlichung
1
Zeitschrift
JOURNAL OF ETHNOPHARMACOLOGY
Schlüsselwörter
  • SH-SY5Y
  • Oxidative stress
  • Apoptosis
  • Neuroprotection
  • Coptidis rhizoma
  • Thioredoxin-interacting protein
Paginierung
607 - 615
Datum der Veröffentlichung
2014
Status
Published
Titel
<i>Coptis chinensis</i> Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells
Sub types
  • Article
Ausgabe der Zeitschrift
155

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Thomas Friedemann
  • Benjamin Otto
  • Kristin Klätschke
  • Udo Schumacher
  • Yi Tao
  • Alexander Kai-Man Leung
  • Thomas Efferth
  • Sven Schröder
DOI
10.1016/j.jep.2014.06.004
ISSN
0378-8741
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of Ethnopharmacology
Sprache
en
Paginierung
607 - 615
Datum der Veröffentlichung
2014
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jep.2014.06.004
Datum der Datenerfassung
2023
Titel
Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells
Ausgabe der Zeitschrift
155

Data source: Crossref

Abstract
<h4>Ethnopharmacological relevance</h4>The dried rhizome of Coptis chinensis Franch. (family Ranunculaceae) is traditionally used in Chinese medicine for the treatment of inflammatory diseases and diabetes. Recent studies showed a variety of activities of Coptis chinensis Franch. alkaloids, including neuroprotective, neuroregenerative, anti-diabetic, anti-oxidative and anti-inflammatory effects. However, there is no report on the neuroprotective effect of Coptis chinensis Franch. watery extract against tert-butylhydroperoxide (t-BOOH) induced oxidative damage. The aim of the study is to investigate neuroprotective properties of Coptis chinensis Franch. rhizome watery extract (CRE) and to evaluate its potential mechanism of action.<h4>Materials and methods</h4>Neuroprotective properties on t-BOOH induced oxidative stress were investigated in SH-SY5Y human neuroblastoma cells. Cells were pretreated with CRE for 2 h or 24 h followed by 2 h of treatment with t-BOOH. To evaluate the neuroprotective effect of CRE, cell viability, cellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and the apoptotic rate were determined and microarray analyses, as well as qRT-PCR analyses were conducted.<h4>Results</h4>Two hours of exposure to 100 µM t-BOOH resulted in a significant reduction of cell viability, increased apoptotic rate, declined mitochondrial membrane potential (MMP) and increased ROS production. Reduction of cell viability, increased apoptotic rate and declined mitochondrial membrane potential (MMP) could be significantly reduced in cells pretreated with CRE (100 µg/ml) for 2h or 24h ahead of t-BOOH exposure with the greatest effect after 24h of pretreatment; however ROS production was not changed significantly. Furthermore, microarray analyses revealed that the expressions of 2 genes; thioredoxin-interacting protein (TXNIP) and mitochondrially encoded NADH dehydrogenase 1, were significantly regulated. Down regulation of TXNIP was confirmed by qRT-PCR.<h4>Conclusion</h4>Due to its neuroprotective properties CRE might be a potential therapeutic agent for the prevention or amelioration of diseases like diabetic neuropathy and neurodegenerative disorders like Alzheimer and Parkinsons disease.
Addresses
  • HanseMerkur Center for Traditional Chinese Medicine at the University Clinic Hamburg-Eppendorf, Martinistr. 52, Hamburg 20246, Germany. Electronic address: Friedemann@tcm-am-uke.de.
Autoren
  • Thomas Friedemann
  • Benjamin Otto
  • Kristin Klätschke
  • Udo Schumacher
  • Yi Tao
  • Alexander Kai-Man Leung
  • Thomas Efferth
  • Thomas Efferth
  • Sven Schröder
DOI
10.1016/j.jep.2014.06.004
eISSN
1872-7573
Externe Identifier
  • PubMed Identifier: 24929105
Funding acknowledgements
  • Innovationsstiftung Hamburg:
Open access
false
ISSN
0378-8741
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of ethnopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Coptis
  • Rhizome
  • Neuroblastoma
  • Reactive Oxygen Species
  • tert-Butylhydroperoxide
  • Neuroprotective Agents
  • Plant Extracts
  • Microarray Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Apoptosis
  • Cell Survival
  • Oxidative Stress
  • Time Factors
  • Membrane Potential, Mitochondrial
Sprache
eng
Medium
Print-Electronic
Online publication date
2014
Paginierung
607 - 615
Datum der Veröffentlichung
2014
Status
Published
Datum der Datenerfassung
2014
Titel
Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
155

Data source: Europe PubMed Central

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The dried rhizome of Coptis chinensis Franch. (family Ranunculaceae) is traditionally used in Chinese medicine for the treatment of inflammatory diseases and diabetes. Recent studies showed a variety of activities of Coptis chinensis Franch. alkaloids, including neuroprotective, neuroregenerative, anti-diabetic, anti-oxidative and anti-inflammatory effects. However, there is no report on the neuroprotective effect of Coptis chinensis Franch. watery extract against tert-butylhydroperoxide (t-BOOH) induced oxidative damage. The aim of the study is to investigate neuroprotective properties of Coptis chinensis Franch. rhizome watery extract (CRE) and to evaluate its potential mechanism of action. MATERIALS AND METHODS: Neuroprotective properties on t-BOOH induced oxidative stress were investigated in SH-SY5Y human neuroblastoma cells. Cells were pretreated with CRE for 2 h or 24 h followed by 2 h of treatment with t-BOOH. To evaluate the neuroprotective effect of CRE, cell viability, cellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and the apoptotic rate were determined and microarray analyses, as well as qRT-PCR analyses were conducted. RESULTS: Two hours of exposure to 100 µM t-BOOH resulted in a significant reduction of cell viability, increased apoptotic rate, declined mitochondrial membrane potential (MMP) and increased ROS production. Reduction of cell viability, increased apoptotic rate and declined mitochondrial membrane potential (MMP) could be significantly reduced in cells pretreated with CRE (100 µg/ml) for 2h or 24h ahead of t-BOOH exposure with the greatest effect after 24h of pretreatment; however ROS production was not changed significantly. Furthermore, microarray analyses revealed that the expressions of 2 genes; thioredoxin-interacting protein (TXNIP) and mitochondrially encoded NADH dehydrogenase 1, were significantly regulated. Down regulation of TXNIP was confirmed by qRT-PCR. CONCLUSION: Due to its neuroprotective properties CRE might be a potential therapeutic agent for the prevention or amelioration of diseases like diabetic neuropathy and neurodegenerative disorders like Alzheimer and Parkinsons disease.
Date of acceptance
2014
Autoren
  • Thomas Friedemann
  • Benjamin Otto
  • Kristin Klätschke
  • Udo Schumacher
  • Yi Tao
  • Alexander Kai-Man Leung
  • Thomas Efferth
  • Sven Schröder
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/24929105
DOI
10.1016/j.jep.2014.06.004
eISSN
1872-7573
Ausgabe der Veröffentlichung
1
Zeitschrift
J Ethnopharmacol
Schlüsselwörter
  • Apoptosis
  • Coptidis rhizoma
  • Neuroprotection
  • Oxidative stress
  • SH-SY5Y
  • Thioredoxin-interacting protein
  • Apoptosis
  • Cell Line, Tumor
  • Cell Survival
  • Coptis
  • Humans
  • Membrane Potential, Mitochondrial
  • Microarray Analysis
  • Neuroblastoma
  • Neuroprotective Agents
  • Oxidative Stress
  • Plant Extracts
  • Reactive Oxygen Species
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhizome
  • Time Factors
  • tert-Butylhydroperoxide
Sprache
eng
Country
Ireland
Paginierung
607 - 615
PII
S0378-8741(14)00438-3
Datum der Veröffentlichung
2014
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2015
Titel
Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
155

Data source: PubMed

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